Uninduced Cu-Metallothionein and Other Cu-Binding Proteins in Mammals and Fish
Among species of metallothionein (Mt), the least understood are those containing copper. Relatively few reports on native Cu-Mt, isolated from organisms not exposed to Cu. Furthermore, there has been marked variability in reported Cu to protein stoichiometry and in the coordination number for sulfhydryl groups bound to Cu1–3. Hence, the experiments reported here focus on basic inorganic properties of a Cu,Zn-Mt isolated from bovine calf liver and survey fresh water fishes for the presence and characteristics of Cu,Zn-binding proteins (BP), analogous to Mt.