Genetic and Biochemical Analysis of Copper Transport in Escherichia coli
Copper is an essential trace element yet it is also potentially toxic. Complex cellular Cu management systems should exist to ensure that supply of Cu to Cu-dependent enzymes occurs without associated toxicity. We predict that these Cu management systems should include: (i) uptake, (ii) intracellular transport and storage, (iii) repair and nullification of Cu damage, (iv) efflux, (v) regulation of cellular Cu levels. Our aim is to use a genetic approach in the microorganism Escherichia coli to investigate these systems. The findings in E. coli will provide leads for research in mammalian systems.