Application of Polarized Luminescence in Biology and Medicine

  • László Szalay
  • Gábor Laczkó
  • Péter Maróti


Our purpose is to provide a brief report on the recent developments and trends in the field and to illustrate their manysided usefulness in diverse topics by many examples, mainly from the literature of the past two years. More details can be found in the books of Steiner (1983), Bayley and Dale (1985) and Lakowicz (1983).


Rotational Diffusion Rotational Correlation Time Anisotropy Decay Main Phase Transition Rotational Relaxation Time 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Alcala, J.R., Gratton, E., and Jameson, D.M., 1985, A multifrequency phase fluorometer using the harmonic content of a mode-locked laser,Anal. Instrum., 14:225.CrossRefGoogle Scholar
  2. 2.
    Amler, E., Teisinger, J., Svobodova, J., and Vyskocil, F., 1986, Vanadyl ions increase the order parameter of plasma membranes without changing the rotational relaxation time,Biochim. Biophys. Acta, 863:18.PubMedCrossRefGoogle Scholar
  3. 3.
    Bayley, P.M., and Dale, R.E., 1985, “Spectroscopy and the dynamics of molecular biological systems”, Academic Press, London.Google Scholar
  4. 4.
    Brasitus, T.A., Dudeja, P.K., Worman, H.J., and Foster, E.S., 1986, The lipid fluidity of rat colonic brush-border membrane vesicles modulates Na+ -H+ exchange and osmotic water permeability,Biochim. Biophys. Acta, 855:16.PubMedCrossRefGoogle Scholar
  5. 5.
    Chester, D.W., Tourtellotte, M.E., Melchior, D.L., and Romano, A.H., 1986, The influence of saturated fatty acid modulation of bilayer physical state on cellular and membrane structure and function,Biochim. Biophys. Acta, 960:383.Google Scholar
  6. 6.
    Chong, L.-G., and Thompson, T.E., 1986, Depolarization of dehydroergosterol in phospholipid bilayers,Biochim. Biophys. Acta, 863:53.PubMedCrossRefGoogle Scholar
  7. 7.
    Dimitropoulos, K., Rolland, J.M., and Nairn, R.C., 1986, Flow cytofluori- metry of fluorescein fluorescence polarization to assay lymphocyte activation,Biochem. Biophys. Res. Commun., 136:1021.PubMedCrossRefGoogle Scholar
  8. 8.
    Faucon, J.F., and Lakowicz, J.R., 1987, Anisotropy decay of dephenylhexa- triene in melittin-phospholipid complexes by multifrequency phase- -modulation fluorometry,Arch. Biochem. Biophys., 252:245.PubMedCrossRefGoogle Scholar
  9. 9.
    Genz, A., and Holzwarth, J.F., 1986, Dynamic fluorescence measurements on the main phase transition of dipalmytoylphosphatidylcholine vesicles,Eur. Biophys. J., 13:323.PubMedCrossRefGoogle Scholar
  10. `10.
    Gratton, E., and Limkeman, M., 1983, A continuously variable frequency cross- -correlation phase fluorometer with picosecond resolution,Biophvs. J., 44:315.CrossRefGoogle Scholar
  11. 11.
    Hönes, G., Hones, J., and Hauser, M., 1986, Studies of enzyme-ligand complexes using dynamic fluorescence anisotropy I-II,Biol. Chem. Hoppe-Seyler, 367:95.PubMedCrossRefGoogle Scholar
  12. 12.
    Ichiye, T., and Karplus, M., 1983, Fluorescence depolarization of tryptophan residues in proteins: a molecular dynamics study,Biochemistry, 22:2884.PubMedCrossRefGoogle Scholar
  13. 13.
    Kapitulnik, J., Weil, E., and Rabinowitz, R., 1986, Glucocorticoids increase the fluidity of the fetal-rat liver microsomal membrane in the perinatal period,Biochem. J., 239:41.PubMedGoogle Scholar
  14. 14.
    Kask, P., Piksarv, P., Mets, U., Pooga, M., and Lippmaa, E., 1987, Fluorescence correlation spectroscopy in the nanosecond time range: rotational diffusion of bovine carbanic anhydrase B,Eur. Biophys. J., 14:257.PubMedCrossRefGoogle Scholar
  15. 15.
    Knutson, J.R., Beechem, J.M., and Brand, L., 1983, Simultaneous analysis of multiple fluorescence decay curves: a global approach,Chem. Phys. Lett., 102:501.CrossRefGoogle Scholar
  16. 16.
    Kwok, F., Kerry, J.A., and Churchich, J.E., 1986, Sheep brain pyridoxal kinase: fluorescence spectroscopy of the dimeric enzyme,Biochim. Biophys. Acta, 874:167.PubMedCrossRefGoogle Scholar
  17. 17.
    Lakowicz, J.R., 1983, “Principles of fluorescence spectroscopy”, Plenum, New York.Google Scholar
  18. 18.
    Lakowicz, J.R., Laczkó, G., Cherek, H., Gratton, E., and Limkeman, M., 1984, Analysis of fluorescence decay kinetics from variable-frequency phase shift and modulation data,Biophys. J., 46:463.PubMedCrossRefGoogle Scholar
  19. 19.
    Lakowicz,J.R.,and Maliwal,B.,1985,Construction and performance of a variable-frequency phase-modulation fluorometer,Biophys.Chem.,21:61.PubMedCrossRefGoogle Scholar
  20. 20.
    Lakowicz, J.R., Laczkó, G., and Gryczynski, I., 1986, 2-GHz frequency- -domain fluorometer,Rev. Sci. Instrum., 57:2499.CrossRefGoogle Scholar
  21. 21.
    Lakowicz, 3.R., Laczkó, G., and Gryczynski, I., 1987, Picosecond resolution of tyrosine fluorescence and anisotropy decays by 2-GHz frequency- -domain fluorometry,Biochemistry, 26:82.PubMedCrossRefGoogle Scholar
  22. 22.
    Laskay, G., and Lehoczki, E., 1986, Correlation between linolenic-acid deficiency in chloroplast membrane lipids and decreasing photosynthetic activity in barley,Biochim. Biophys. Acta, 849:77.CrossRefGoogle Scholar
  23. 23.
    Levy, R.M., and Szabó, A., 1982, Initial fluorescence depolarization of tyrosine in proteins,J. Am. Chem. Soc., 104:2073.CrossRefGoogle Scholar
  24. 24.
    Nordlund, T.M., Liu, X-Y., and Sommer, 3.H., 1986, Fluorescence polarization decay of tyrosine in lima bean trypsin inhibitor,Proc. Natl. Acad. Sci. USA, 83:8977.PubMedCrossRefGoogle Scholar
  25. 25.
    O’Connor, D.V., and Phillips, D., 1984, “Time-correlated single photon counting”, Academic, London.Google Scholar
  26. 26.
    Petrich, 3.W., Longworth, J.W., and Fleming, G.R., 1987, Internal motion and electron transfer in proteins: a picosecond fluorescence study of three homologous azurins,Biochemistry, 26:2711.PubMedCrossRefGoogle Scholar
  27. 27.
    Popp-Snijders, C., Schouten, J.A., van Blitterswijk, W.J., and van der Veen, E.A., 1986, Changes in membrane lipid composition of human erythrocytes after dietary supplementation of (n-3) polyunsaturated fatty acids. Maintenance of membrane fluidity.Biochim. Biophys. Acta, 854:31.PubMedCrossRefGoogle Scholar
  28. 28.
    Sanyal, G., Charlesworth, M.C., Ryan, R.J., and Prendergast, F.G., 1987, Tryptophan fluorescence studies of subunit interaction and rotational dynamics of human luteinizing hormone,Biochemistry, 26:1860.PubMedCrossRefGoogle Scholar
  29. 29.
    Schroeder, F., Barenholz, Y., Gratton, E., and Thompson, T.E., 1987, A fluorescence study of dehydroergosterol in phosphatidylcholine bilayer vesicles,Biochemistry, 26:2441.PubMedCrossRefGoogle Scholar
  30. 30.
    Schwarz, S.M., Watkins, J.B., Ling, S.C., Fayer, J.C., and Mone, M., 1986, Effects of ethinyl estradiol on intestinal membrane structure and function in the rabbit,Biochim. Biophys. Acta, 860:411.PubMedCrossRefGoogle Scholar
  31. 31.
    Sire, 0, Mangeney, M., Montagne, J., Bereziat, G., and Nordmann, J., 1986, Changes of fatty acid composition of phospholipids and lipid structural order in rat liver mitochondrial membrane subsequent to galactos- amine intoxication. Effect of clofibrate,Biochim. Biophys. Acta, 860:75.PubMedCrossRefGoogle Scholar
  32. 32.
    Steiner, R.F., ed., 1983, “Excited states of biopolymers”, Plenum, New York.Google Scholar
  33. 33.
    Sweet, W.D., Wood, W.G., and Schroeder, F., 1987, Charged anesthetics selectively alter plasma membrane order,Biochemistry, 26:2828.PubMedCrossRefGoogle Scholar
  34. 34.
    Van Blitterswijk, W.J., van der Meer, B.W., and Hilkmann, H., 1987, Quantitative contributions of cholesterol and the individual classes of phospholipids and their degree of fatty acyl (un)saturation to membrane fluidity measured by fluorescence polarization,Biochemistry, 26:1746.PubMedCrossRefGoogle Scholar
  35. 35.
    Van der Meer, B.W., van Hoeven, R.P., and van Blitterswijk, W.J., 1986, Steady-state fluorescence polarization data in membranes. Resolution into physical parameters by an extended Perrin equation for restricted rotation of fluorophores,Biochim. Biophys. Acta, 854:38.PubMedCrossRefGoogle Scholar
  36. 36.
    Van de Waart, P., Visser, A.J.W.G., Hemker, H.C., and Lindhout, T., 1987, The effect of factor Va on lipid dynamics in mixed phospholipid vesicles as detected by steady-state and time-resolved fluorescence depolarization of diphenylhexatriene,Eur. J. Biochem., 164:337.PubMedCrossRefGoogle Scholar
  37. 37.
    Wasylewski, Z., Stryjewski, W., Wasniowska, A., Potempa, J., and Baran, K., 1986, Effect of calcium binding on conformational changes of staphylococcal metalloproteinase measured by means of intrinsic protein fluorescence,Biochim. Biophys. Acta, 871:177.PubMedCrossRefGoogle Scholar
  38. 38.
    Wood, W.G., Gorka, C., Williamson, L.S., Strong, R., Sun, A.Y., Sun, G.Y., and Schroeder, F., 1986, Dolichol alters dynamic and static properties of mouse synaptosomal plasma membranes,FEBS Lett, 205:25.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • László Szalay
    • 1
  • Gábor Laczkó
    • 1
  • Péter Maróti
    • 1
  1. 1.Department of BiophysicsJózsef Attila UniversityHungary

Personalised recommendations