Higher Order Chromatin Structure in Transcriptionally Active and Inactive Cells
At present there is no general view on the organization of nucleosome chain packaging in the so called 30nm chromatin fiber. Comparative studies of chromatin differing in transcriptional activity and in linker DNA lengths represent a fruitful approach to the problem of higher order chromatin structure (Osipova et al., 1980; 1986; Zalenskaya et al., 1981; Thomas et al., 1986). This communication reports the analysis of the sedimentation data for chromatin from nuclei of pigeon brain cortical neurones, rat thymus and sea urchin sperm, characterized by linker DNA sizes of 20, 50, and 100 base pairs, respectively.
KeywordsIonic Strength Sedimentation Coefficient Cylinder Model Sedimentation Behaviour Sperm Chromatin
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- Osipova T. N., 1987, Structural parameters of short DNA fragments and oligonucleosomes on the basis of the cylinder model, Studia Biophysica, 120:171.Google Scholar
- Osipova T. N., Karpova E. V., Svetlikova S. B., Kukushkin A. N. and Pospelov V. A., 1986, Structure of pigeon brain chromatin: sedimentation of analysis of oligonucleosome compaction, Molekul. Biol., 20:78.Google Scholar
- Ramm E. I., Ivanov G. S., Pospelov V. A., Svetlikova S. B. and Kukushkin A.N. 1986, Peculiarities of structural state of DNA in chromatin with short linker, Biofizika, 31:404.Google Scholar