Abstract
DNA-associated proteins of spermatozoa are different from somatic cell histones. In mammals, arginine-rich protamines replace histones and transition proteins in spermatids during compaction of the chromatin (Poccia, 1986). The mouse protamine mRNAs are expressed in early spermatids by a haploid genome (Kleene et al., 1983). To find the exact stages of differentiation where the expression of spermatidal nucleoprotein mRNAs occur, we used three different cDNA-hybridization techniques to measure protamine 1, protamine 2 and transition protein (TP1) mRNA levels.
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References
Chirgwin J. M., Przybyla A. R., MacDonald R. J., and Rutter W. J. 1979. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry, 18:5294.
Clermont Y., and Morgentaler H. 1955. Quantitative study of spermatogenesis in the hypophysectomized rat. Endocrinology 57:369.
Dym M., and Clermont Y. 1970. Role of spermatogonia in the repair of the seminiferous epithelium following X-irradiation of the rat testis. Am. J. Anat. 128:265.
Kangasniemi M., Kaipia A., Mali P., Toppari J., Huhtaniemi I., Parvinen I., and M. 1989. Modulation of basal and FSH-stimulated cyclic AMP production in rat seminiferous tubules staged by an improved transillumination technique. Anat. Rec. (in press).
Kierszenbaum A. L., and Tres L. L. 1985. Structural and transcriptional features of the mouse spermatid genome. J. Cell Biol. 65:258.
Kleene K. C., Distel R. J. and Hecht N. B. 1983. cDNA clones encoding cytoplasmic poly(A) RNAs which first appear at detectable levels in haploid phases of spermatogenesis in the mouse. Dev. Biol. 98:455.
Kleene K. C., Distel R. J. and Hecht N. B. 1984. Translational regulation and deadenylation of a protamine mRNA during spermiogenesis in the mouse. Dev. Biol. 105:71-
Meistrich M. L., Brock W. A., Grimes S. R., Platz R. D., and Hnilica L. S. 1978. Nuclear protein transitions during spermatogenesis. Federation Proc. 37:2522.
Monesi V. 1964. Ribonucleic acid synthesis during mitosis and meiosis in the mouse testis. J. Cell Biol. 22:521.
Parvinen M., and Ruokonen A. 1982. Endogenous steroids in rat seminiferous tubules. Comparison of the stages of the epithelial cycle isolated by transillumination-assisted microdissection. J. Androl. 3:211.
Poccia D. 1986. Remodeling of nucleoproteins during gametogenesis, fertilization and early development. Int. Rev. Cytol. 105:1.
Sandberg M., and Vuorio E. 1987. Localization of types I, II and III collagen mRNAs in developing human skeletal tissues by in situ hybridization. J. Cell. Biol. 104:1077.
Thomas P. S. 1980. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc. Natl. Acad. Sci. USA, 77:5201.
Toppari J., Eerola E., and Parvinen M. 1985. Flow cytometric DNA analysis of defined stages of rat seminiferous epithelial cycle during in vitro differentiation. J. Androl. 6:325.
Toppari J., and Parvinen M. 1985. In vitro differentiation of rat seminiferous tubular segments from defined stages of the epithelial cycle: morphologic and immunolocalization analysis. J. Androl. 6:334.
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© 1990 Plenum Press, New York
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Mali, P. et al. (1990). Expression of Nucleoprotein mRNAs During Rat Spermiogenesis. In: Harris, J.R., Zbarsky, I.B. (eds) Nuclear Structure and Function. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0667-2_18
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DOI: https://doi.org/10.1007/978-1-4613-0667-2_18
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