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Purification and Characterization of Bacillus Cereus Enterotoxin and its Application to Diagnosis

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Microbial Toxins in Foods and Feeds

Abstract

Bacillus cereus is a large, gram-positive, spore-forming rod, and is aerobic or facultatively anaerobic. It is distributed widely in the environment including different kinds of foods and may cause food poisoning. Two types of B. cereus food poisoning are known;2 the diarrheal type with an incubation period of 8 to 16 hours and the vomiting type with an incubation period of 1 to 5 hours. The diarrheal type characterized by diarrhea and abdominal pain resembles Clostridium perfringens food poisoning. The vomiting type is characterized by nausea and vomiting and resembles staphylococcal enterotoxin food poisoning. The agents of diarrheal type and vomiting type are toxins designated as “diarrheal toxin” (or enterotoxin) and “emetic toxin, ” respectively. Diagnosis of B. cereus food poisoning by the detection of the toxin in clinical specimens or in incriminated foods has not been established.

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References

  1. Ezepchuk YV, Bondarenko VM, Yakovleva EA, Koryagina IP, 1979, The Bacillus cereus toxin: isolation of permeability factor, Zentralbl Bakteriol Hyg., I Abt Orig A, 244:275–284.

    CAS  Google Scholar 

  2. Gilbert RJ, 1979, Bacillus cereus gastroenteritis, In: Foodborne infections and intoxications, 2nd ed., Riemann H, Bryan FL, eds., Academic Press, New York.

    Google Scholar 

  3. Glatz BA, Goepfert JM, 1973, Extracellular factor synthesized by Bacillus cereus which evokes a dermal reaction in guinea pigs, Infect Immun., 8:25–29.

    CAS  Google Scholar 

  4. Glatz BA, Spira, WM, Goepfert, JM, 1974, Alteration of vascular permeability in rabbits by culture filtrates of Bacillus cereus and related species, Infect Immun., 10:299–303.

    CAS  Google Scholar 

  5. Glatz BA, Goepfert JM, 1976, Defined conditions for synthesis of Bacillus cereus enterotoxin by fermenter-grown cultures, Appl Environ Microbiol., 32:400–404.

    CAS  Google Scholar 

  6. Glatz BA, Goepfert, JM, 1977, Production of Bacillus cereus enterotoxin in defined media in fermenter-grown cultures, J Food Prot., 40:472–474.

    CAS  Google Scholar 

  7. Goepfert JM, 1974, Monkey feeding trials in the investigation of the nature of Bacillus cereus food poisoning, In: Proc Fourth International Congress of Food Science and Technology, Vol 3, Institute Nacional de Ciencia Y Tecnoogia de Alimentos, Consejo Seuperior de Investigaciones Cientificas, Madrid.

    Google Scholar 

  8. Hauge S, 1955, Food poisoning caused by aerobic spore - forming bacilli, J Appl Bacteriol., 18:591–595.

    Google Scholar 

  9. Kramer JM, Gilbert RJ, 1988, Bacillus cereus and other Bacillus species, In: Foodborne bacterial pathogens, Doyle, MP, ed., Marcal Dekker, New York.

    Google Scholar 

  10. Melling J, Capel BJ, Turnbull PCB, Gilbert RJ, 1976, Identification of a novel enterotoxigenic activity associated with Bacillus cereus, J Clin Pathol., 29:938–940.

    Article  CAS  Google Scholar 

  11. Ouchterlony 0, 1949, Antigen-antibody reaction in gel, Acta Pathol Microbiol Scand., 26:507–517.

    Article  CAS  Google Scholar 

  12. Public Health Laboratory Service, 1972, Food poisoning associated with Bacillus cereus, Brit Med J., 1:189.

    Google Scholar 

  13. Shinagawa K, Kunita N, Sasaki Y, Okamoto A, 1979, Biochemical characteristics and heat tolerance of strains of Bacillus cereus isolated from uncooked and cooked rice after food poisoning outbreaks, (In Japanese, English summary), J Food Hyg Soc., Japan 2.0:431–436.

    Google Scholar 

  14. Shinagawa K, Matsusaka N, Konuma H, Kurata H, 1985, The relation between the diarrheal and other biological activities of Bacillus cereus involved in food poisoning outbreaks, Jpn J Vet Sci47: 557–565.

    Article  CAS  Google Scholar 

  15. Spira WM, Goepfert JM, 1972, Bacillus cereus-induced fluid accumulation in rabbit ileal loops, Appl Microbiol., 24:341–348.

    CAS  Google Scholar 

  16. Spira WM, Goepfert, JM, 1975, Biological characteristics of an enterotoxin produced by Bacillus cereus, Can J Microbiol., 21: 1236–1246.

    Article  CAS  Google Scholar 

  17. Thompson NE, Ketterhagen MJ, Bergdoll MS, Schantz EJ, 1984, Isolation and some properties of an enterotoxin produced by Bacillus cereus, Infect Immun., 43:887–894.

    CAS  Google Scholar 

  18. Turnbull PCB, 1981, Bacillus cereus toxins, Pharmacol Ther., 13: 453–505.

    Article  CAS  Google Scholar 

  19. Turnbull PCB, Kramer JM, Jorgensen K, Gilbert RJ, Melling J, 1979, Properties and production characteristics of vomiting, diarrheal, and necrotozing toxins of Bacillus cereus, Amer J Clin Nutr., 32:219–228.

    CAS  Google Scholar 

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© 1990 Plenum Press, New York

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Shinagawa, K. (1990). Purification and Characterization of Bacillus Cereus Enterotoxin and its Application to Diagnosis. In: Pohland, A.E., et al. Microbial Toxins in Foods and Feeds. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0663-4_18

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  • DOI: https://doi.org/10.1007/978-1-4613-0663-4_18

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-7916-7

  • Online ISBN: 978-1-4613-0663-4

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