Involvement of Calcium in the Transduction of the Hormonal Signal for Induction of Oocyte Maturation

  • Nava Dekel
  • Sari Goren
  • Yoram Oron


The mammalian female is born with her ovaries containing the entire pool of oocytes, all of them arrested at the diplotene of the first meiotic prophase. Meiotic arrest in the oocytes persists throughout infancy. After the onset of puberty, at each cycle, one or more oocytes respond to the midcycle surge of luteinizing hormone (LH) and resume meiosis. Oocytes that resumed meiosis had generated an haploid nucleus and acquired the capacity to be fertilized. Resumption of meiosis is therefore referred to as oocyte maturation.


Luteinizing Hormone Oocyte Maturation Mammalian Oocyte Meiotic Arrest Graafian Follicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aberdam, E., and Dekel, N., 1985, Activators of protein kinase C stimulate meiotic maturation of rat oocytes. Biochem Biophys. Res. Common., 132:570.CrossRefGoogle Scholar
  2. Allen, R.B., Su, H.C., Snitzer, J., Dimino, M.J., 1988, Rapid decreases in phosphatidylinositol in isolated luteal plasma membranes after stimulation by luteinizing hormone. Biol. Reprod., 38:79.PubMedCrossRefGoogle Scholar
  3. Bae, I., Channing, C.P., 1985, Effect of calcium ion on the maturation of cumulus-enclosed pig follicular oocytes isolated from medium-sized Graafian follicles. Biol. Reprod., 33:79.PubMedCrossRefGoogle Scholar
  4. Baulieu, E.E., Godeau, F., Schorderet, M., and Schorderet-Slatkine, S., 1978, Steroid-induced meiotic division in Xenopus laevis oocytes: surface and calcium. Nature, 275:593.PubMedCrossRefGoogle Scholar
  5. Clayton, R.N., Harwood, J.P., and Catt, K.J., 1979, Gonadotropin-releasing hormone analogue binds to luteal cells and inhibits progesterone production. Nature, 282:90.PubMedCrossRefGoogle Scholar
  6. Corbin, A., and Bex, F.J., 1981, Luteinizing hormone-releasing hormone agonists induce ovulation in hypophysectomized proestrous rats: direct ovarian effect. Life. Sci., 29:185.PubMedCrossRefGoogle Scholar
  7. Davis, J.S., Weakland, L.L., West, L.A., and Farese, R.V., 1986, Luteinizing hormone stimulates the formation of inositol triphosphate and cyclic AMP in rat granulosa cells. Biochem. J., 238:597.PubMedGoogle Scholar
  8. Dekel, N., Aberdam, E., Goren, S., Feldman, B., and Shalgi, R., 1989, Mechanism of action of GnRH-induced oocyte maturation. J. Reprod. Fert. Suppl., 37:319.Google Scholar
  9. Dekel, N., Lewysohn, O., Ayalon, D., and Hazum, E., 1988, Receptors for gonadotropin releasing hormone are present in rat oocytes. Endocrinology, 123:120.CrossRefGoogle Scholar
  10. Dekel, N., Sherizly, I., Phillips, D.M., Nimrod, A., Zilberstein, M., and Naor, Z., 1985, Characterization of the maturational changes induced by a GnRH analogue in the rat ovarian follicle. J. Reprod. Fert., 75:461.CrossRefGoogle Scholar
  11. Dekel, N., Sherizly, I., Tsafriri, A., and Naor, Z., 1983, A comparative study of the mechanism of action of luteinizing hormone and gonadotropin-releasing hormone analog on the ovary. Biol. Reprod., 28:161.PubMedCrossRefGoogle Scholar
  12. Dimino, M.J., Snitzer, J., and Brown K.M., 1987, Inositol phosphates accumulation in ovarian granulosa after stimulation by luteinizing hormone. Biol. Reprod., 37:1129.PubMedCrossRefGoogle Scholar
  13. Eckstein, N., Eshel, A., Eli, Y., Ayalon, D., and Naor, Z. 1986, Calcium-dependent actions of gonadotropin-releasing hormone agonist and luteinizing hormone upon cyclic AMP and progesterone production in rat ovarian granulosa cells. Mol. Cell. Endocr. 47:91.CrossRefGoogle Scholar
  14. Ekholm, C., Hillensjo, T., and Isaksson, O. 1981, Gonadotropin-releasing hormone agonists stimulate oocyte meiosis and ovulation in hypophysectomized rats. Endocrinology, 108:2022.PubMedCrossRefGoogle Scholar
  15. Garcia, A.G., Sala, F., Reig, J.A., Viniegra, S., Frias, J., Fonteriz, R., and Gandia, L. 1984, Dihydropyridine BAY-K-8644 activates chromaffin cell calcium channels. Nature, 309:69.PubMedCrossRefGoogle Scholar
  16. Godfraind, T„ 1984, Calcium modulators: Tools for cell biology, in: “Advances Cyclic Nucleotide and Protein Phosphorylation Research”, Greengard, P., ed., Vol. 17, p 5.Google Scholar
  17. Harris, C.E., Staley, D., and Conn, P.M., 1985, Diacylglycerol and protein kinase C: Potential amplifying mechanism for Ca2+-mediated GnRH-stimulated LH release. MoL Pharmacol., 27:532.PubMedGoogle Scholar
  18. Hidaka, H., Inagaki, M., Kawamoto, S., and Sasaki, Y. 1984, Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide-dependent protein kinase and protein kinase C. Biochemistry, 23:5036.PubMedCrossRefGoogle Scholar
  19. Hillensjö, T., and LeMaire, W.J., 1980, Gonadotropin-releasing hormone agonists stimulate meiotic maturation of follicle-enclosed rat oocytes in vitro. Nature, London 287:145.CrossRefGoogle Scholar
  20. Hirota, K., Hirota, T., Aguilera, G., and Catt, K.J., 1985, Hormone-induced redistribution of calcium-activated phospholipid-dependent protein kinase in pituitary gonadotrophs. /. Biol. Chem. 260:3243.Google Scholar
  21. Jagiello, G., Ducayen, M.B., Downey, R., and Jonassen, A., 1982, Alternations of mammalian oocyte meiosis I with divalent cations and calmodulin. Cell. Calcium 3:153.PubMedCrossRefGoogle Scholar
  22. Jones, P.B. C„ Conn, P.M., Marian, J., and Hsueh, A.J.W., 1980, Binding of gonadotropinreleasing hormone agonist to rat ovarian granulosa cells. Life. Sci. 27:2125.PubMedCrossRefGoogle Scholar
  23. Klee, C.B., Crouch, T.H., and Richman, P.G. 1980, Calmodulin. Ann. Rev. Biochem., 49:489.PubMedCrossRefGoogle Scholar
  24. Leibfried, L., and First, N.L., 1979, Effects of divalent cations on in vitro maturation of bovine oocytes. J. Exp. Zool. 210:575.PubMedCrossRefGoogle Scholar
  25. Lindner, H.R., Tsafriri, A., Lieberman, M.E., Zor, U., Koch, Y., Bauminger, S., and Barnea, A., 1974, Gonadotrophin action on cultured Graafian follicles: induction of maturation division of the mammalian oocyte and differentiation of the luteal cell. Recent Prog. Horm. Res., 30:79.PubMedGoogle Scholar
  26. Maruska, D.V., Leibfried, M.L., and First, N.L., 1984, Role of calcium and the calciumcalmodulin complex in resumption of meiosis, cumulus expansion, viability and hyaluronidase sensitivity of bovine cumulus-oocyte complexes. Biol. Reprod., 31:1.PubMedCrossRefGoogle Scholar
  27. Masui, Y., and Clarke, H.J., 1979, Oocyte maturation. Int. Rev. Cytol. 7:185.CrossRefGoogle Scholar
  28. Morrill, G.A., and Kostellow, A.B., 1986, The role of calcium in meiosis. Calcium and Cell Function, 6:209.Google Scholar
  29. Naor, Z., Zer, J., Zakut, H., and Hermon, J., 1985, Characterization of pituitary calciumactivated, phospholipid-dependent protein kinase: redistribution by gonadotropin-releasing hormone. Proc. Natl. Acad. Sci. U.S.A., 82:8203.PubMedCrossRefGoogle Scholar
  30. Neher, E., 1987, Receptor-operated Ca channels. Nature 326:242.PubMedCrossRefGoogle Scholar
  31. Paieos, G.A., and Powers, R.D., 1981, The effect of calcium on the first meiotic division of the mammalian oocyte. J. Exp. Zool. 217:409.CrossRefGoogle Scholar
  32. Racowsky, C. 1986, The releasing action of calcium upon cyclic AMP-dependent meiotic arrest in hamster oocytes. J. Exp. Zool, 239:263.PubMedCrossRefGoogle Scholar
  33. Reuter, H., 1985, A variety of calcium channels. Nature, 316:391.PubMedCrossRefGoogle Scholar
  34. Tsafriri, A., Lindner, H.R., and Zor, U., 1972, In vitro induction of meiotic division in follicleenclosed rat oocytes by LH, cAMP and PGE2. Reprod. Fert. 31:39.CrossRefGoogle Scholar
  35. Tsafriri, A., and Bar-Ami, S., 1978, Role of divalent cations in the resumption of meiosis of rat oocytes. J. Exp. Zool. 205:293.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Nava Dekel
    • 1
  • Sari Goren
    • 1
  • Yoram Oron
    • 2
  1. 1.Department of Hormone ResearchThe Weizmann Institute of ScienceTel AvivIsrael
  2. 2.Department of Physiology and PharmacologySackler Faculty of MedicineTel AvivIsrael

Personalised recommendations