Lesion Measurement in Non-Radioactive DNA by Quantitative Gel Electrophoresis
The important role of DNA damage and repair in homeostasis has been clearly demonstrated by studies involving isolated DNA, procaryotic cells and eukaryotic cells growing in culture. The information obtainable from these model systems, while extremely valuable, can never replace the need for data from intact higher organisms. The gel electrophoresis method developed during the past ten years in our laboratories makes possible the quantitation of UV induced pyrimidine dimers, gamma ray induced single- and double-strand breaks and many other types of lesions in nanogram quantities of DNA. The DNA does not have to be labeled with radionuclides or of a particular conformation, thus facilitating the use of the method in measuring damage levels and repair rates in the DNA of intact organisms -- including man.
KeywordsMigration Sucrose Agarose Bromide Ozone
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