Summary
The vacuoles of plant cells contain a variety of proteins including acid hydrolases, storage proteins and plant defense proteins. During seed development, the central vacuoles of the storage parenchyma cells accumulate large amounts of all three classes of these proteins. We have studied the biosynthesis, transport, posttranslational modifications and accumulation in developing legume cotyledons of acid hydrolases (e.g. α-mannosidase), storage proteins (e.g. phaseolin), and plant defense proteins (e.g. phytohemagglutinin and α-amylase inhibitor). Transport of proteins to vacuoles is mediated by the secretory system (endoplasmic reticulum and Golgi apparatus) and correct targeting of protein to vacuoles requires positive sorting information. This information is contained within the polypeptide domain of the vacuolar glycoprotein phytohemagglutinin (PHA). When the gene for PHA is introduced into yeast ( Saccharomyces cerevisiae) cells, the resulting protein is targeted to yeast vacuoles. By expressing in yeast, chimeric genes consisting of the signal peptide and various portions of the PHA coding region with the gene for yeast invertase, we were able to show that the vacuolar targeting domain of PHA is in an amino- proximal region between amino acids 14 and 43 of the mature protein. Experiments are now under way to determine whether the same domain of PHA can target yeast invertase to plant vacuoles (protein bodies in tobacco seeds).
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© 1989 Plenum Press, New York
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Chrispeels, M.J., Tague, B.W. (1989). Transport and Targeting of Proteins to Protein Storage Vacuoles (Protein Bodies) in Developing Seeds. In: Taylorson, R.B. (eds) Recent Advances in the Development and Germination of Seeds. NATO ASI Series, vol 187. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0617-7_11
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DOI: https://doi.org/10.1007/978-1-4613-0617-7_11
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