Abstract
Interaction of the blastocyst with the endometrium at implantation starts with adhesion of the apical membrane of the trophoblast to the apical membranes of the uterine epithelium (Enders and Schlafke, 1972; Schlafke and Enders, 1979). In the rabbit this event is followed by apical membrane fusion of both partner tissues thus leading to the formation of a mixed symplasm, and by invasion into the endometrium (Enders and Schlafke, 1971). At the same time, widespread fusion of the lateral cell membranes of the uterine epithelial cells starts. This uterine epithelial cell fusion occurs in two overlapping phases, in the antimesometrial portion of the endometrium from 7 days post coitum (7 d p.c.) associated with attachment and invasion of the abembryonic trophoblast (yolk sac placentation), and mesometrially on the endometrium of the placental folds from 8 d p.c. (associated with chorioallantoic placentation). Uterine symplasms formed in this way are found exclusively in the implantation chamber, i.e., in the vicinity of a blastocyst, and are very large, containing hundreds of nuclei.
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References
Anderson, T.L., Hoffman, L.H. (1984) Alterations in epithelial glycocalyx of rabbit uteri during early pseudopregnancy and pregnancy, and following ovariectomy. Am. J. Ana..171, 321–334.
Aroeti, B., Henis, Y.I. (1986) The lateral mobility of cell membrane components is not altered following cell fusion induced by Sendai virus. Exp. Cell Re..162, 243–254.
Bearer, E.L., Friend, D.S. (1982) Modifications of anionic-lipid domains preceding membrane fusion in guinea pig sperm. J. Cell Bio..92, 604–615.
Beier, H.M., Kühnel, W. (1973) Pseudopregnancy in the rabbit after stimulation by human chorionic gonadotropin. Hormone Re..4, 1–27.
Busch, L.C., Winterhager, E., Kühnel, W. (1981) Symplasmatische Umwandlung des Cavumepithels im Uterus pseudogravider Kaninchen. Acta Ana..111, 22.
Chandler, D.E. (1984) Comparison of quick-frozen and chemically fixed sea urchin eggs: Structural evidence that cortical granule exocytosis is preceded by a local increase in membrane mobility. J. Cell Sc..72, 23–36.
Citi, S., Sabanay, H., Jakes, R, Geiger, B., Kendrick-Jones, J. (1988) Cingulin, a new peripheral component of tight junctions. Natur.333, 272–276.
Elias, P.M., Friend, D.S., Goerke, J. (1979) Membrane sterol heterogeneity. Freeze-fracture detection with saponins and filipin. J. Histochem. Cytoche..27, 1247–1260.
Elias, P.M., Goerke, J., Friend, D.S. (1978) Freeze-fracture identification of sterol-digi tonin complexes in cell and liposome membranes. J. Cell Bio..577–596.
Enders, A.C. (1975) The implantation chamber, blastocyst and blastocyst imprint of the rat: A scanning electron microscope study. Anat. Re..182, 137–150.
Enders, A.C., Nelson, D.M. (1973) Pinocytotic activity of the uterus of the rat. Am. J. Ana..138, 277–300.
Enders, A.C., Schlafke, S. (1967) A morphological analysis of the early implantation stages in the rat. Am. J. Ana..120, 185–226.
Enders, A.C., Schlafke, S. (1971) Penetration of the uterine epithelium during implantation in the rabbit. Am. J. Ana..132, 219–240.
Enders, A.C., Schlafke, S. (1972) Implantation in the ferret: Epithelial penetration. Am. J. Ana..133, 291–316.
Feltkamp, C.A., van der Waerden, A.W.M. (1982) Membrane associated proteins affect the formation of filipin-cholesterol complexes in viral membranes. Exp. Cell Re..140, 289–297.
Hartmann, W., Galla, H.-J., Sackmann, E. (1978) Polymyxin binding to charged lipid membranes an example of cooperative lipid-protein interaction. Biochim. Biophys. Act.510, 124–139.
Kinsky, S.C., Luse, S.A., Sopf, D., van Deenen, L.L.M., Haxby, J. (1967) Interactions of filipin and derivatives with erythrocyte membranes and lipid dispersions. Biophys. Act.135, 844–861.
Leiser, R. (1979) Blastocystenimplantation bei der Hauskatze. Licht- und elektronenmikroskopische Untersuchung. Zbl. Vet. Med. C. Anat. Histol. Embryo..8, 79–96.
Leiser, R. (1980) Funktionelle Morphologie der Implantation und der frühen Plazentation bei der Hauskatze. Licht- und elektronenmikroskopische Untersuchung mit histocytochemischer Ergänzung. Habilitationsschrift, Institut für Tieranatomie der Universität Bern.
Murphy, CR., Martin, B. (1984) Freeze-fracture cytochemistry with polymyxin B. A study on the plasma membrane of uterine epithelial cells. Histoche..80,327–331.
Murphy, CR., Martin B. (1985) Cholesterol in the plasma membrane of uterine epithelial cells: A freeze-fracture cytochemical study with digitonin. J. Cell Sc..78,163–172.
Murphy, CR., Dwarte, D.M. (1987) Increase in cholesterol in the apical plasma membrane of uterine epithelial cells during early pregnancy in the rat. Acta Ana..128, 76–79.
Murphy, CR., Swift, J.G. (1983) Pronase reduces intramembranous particle density in uterine epithelial cells in vivo. Acta Ana..116, 174–179.
Norman, A.V., Demel, R.A., de Kruijff, B., van Deenen, L.L.M. (1972) Studies on the biological properties of polyene antibiotics. Evidence for the direct interaction of filipin with cholesterol. J. Biol. Che..247, 1918–1929.
Padykula, H.A., Taylor, J.M. (1976) Ultrastructural evidence for loss of the trophoblastic layer in the chorioallantoic placenta of Australian bandicoots (Marsupialia: Peramelidae) Anat. Re..186, 357–386.
Papahadjopoulos, D. (1978) Calcium-induced phase changes and fusion in natural and model membranes. In: Membrane Fusio.,(eds.) G. Poste, G.L. Nicolson, Elsevier: North-Holland Biomedical Press, pp. 765–790.
Schlafke, S., Enders, A.C. (1975) Cellular basis of interaction between trophoblast and uterus at implantation. Biol. Repro..12,41–65.
Severs, N.J., Robenek, H. (1983) Detection of microdomains in biomembranes. An appraisal of recent developments in freeze-fracture cytochemistry. Biochim. Biophys. Act.737, 373–408.
Severs, N.J., Simons, H.L. (1983) Failure of filipin to detect cholesterol rich domain in smooth muscle plasma membrane. Nature (Lond).303, 637–638.
Shinitzky, M. (1984) Membrane fluidity and cellular functions. In: Physiology of Membrane Fluidit.,(ed.) M. Shinitzky, Vol. 1., Boca Raton, Florida: CRC Press Inc.
Sixl, F., Galla, H.-J. (1981) Polymyxin interaction with negatively charged lipid bilayer membranes and the competetive effect of Ca++. Biochim. Biophys. Act.643, 626–635.
Steer, J.C., Bisher, M., Blumenthal, R., Steven, A.C. (1984) Detection of membrane cholesterol by filipin in isolated rat liver coated vesicles is dependent upon removal of the clathrin coat. J. Cell Bio..99, 315–319.
Trier, J.S., Madara, J.L. (1984) Distribution of filipin-sterol complexes in villus goblet cell membranes of rat small intestine. Lab. Inves.50, 673–682.
Verkleij, A.J., de Kruijff, B., Gerritsen, W.F., Demel, R.A., van Deenen, L.L.M., Ververgaert, P.H.J. (1973) Freeze-etch electron microscopy of erythrocytes, Acholeplasma laidlawii cells and liposomal membranes after the action of filipin and amphotericin B. Biochim. Biophys. Act.291, 577–581.
White, J., Helenius, A. (1980) pH-dependent fusion between the Semliki forest virus membrane and liposome. Proc. Natl.Acad. Sci. US.77, 3273–3277.
Winterhager, E. (1985) Dynamik der Zellmembran: Modellstudien während der Implantationsreaktion beim Kaninchen. Habilitationsschrift, Medizinische Fakultä.,RWTH Aachen.
Winterhager, E., Kühnel, W. (1982) Alterations in intercellular junctions of the uterine epithelium during the preimplantation phase in the rabbit. Cell Tissue Re..224, 517–526.
Winterhager, E., Busch, L.C., Kühnel, W. (1984) Membrane events involved in fusion of uterine epithelial cells in pseudopregnant rabbits. Cell Tissue Re..235, 357–363.
Winterhager, E., Brümmer, F., Dermietzel, R, Hülser, D.F., Denker, H.-W. (1988) Gap junction formation in rabbit uterine epithelium in response to embryo recognition. Dev. Bio..126, 203–211.
Wolf, D.E., Kinsey, W., Lennarz, W., Eddin, M. (1981) Changes in the Organization of the sea urchin egg plasma membrane upon fertilization: Indications from the lateral diffusion rates of lipid-soluble fluorescent dyes. Dev. Bio..81, 133–138.
Wooding, F.B.P., Chambers, S.G., Perry, J.S., George, P.M., Heap, R.B. (1980) Migration of binucleate cells in the sheep placenta during normal pregnancy. Anat. Embryo..158, 361–370.
Yanagimachi, R., Noda, Y.D., Fujimoto, M., Nicolson, G.I. (1972). The distribution of negative surface charges on mammalian spermatozoa. Am. J. Ana..135,497–520.
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© 1990 University of Rochester
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Winterhager, E., Denker, HW. (1990). Changes in Lipid Organization of Uterine Epithelial Cell Membranes at Implantation in the Rabbit. In: Denker, HW., Aplin, J.D. (eds) Trophoblast Invasion and Endometrial Receptivity. Trophoblast Research, vol 4. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0615-3_17
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DOI: https://doi.org/10.1007/978-1-4613-0615-3_17
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