Abstract
Two pathways for H2 metabolism have been identified in Escherichia coli and other members of the family Enterobacteriaceae under anaerobic growth conditions. The first pathway, known as the formate hydrogenlyase system, occurs during fermentative growth on carbohydrates in the absence of an external electron acceptor (Peck et Gest, 1957). It consists at least of two enzymes, a benzyl viologen-linked formate dehydrogenase (FDH-BV) and a hydrogenase, which catalyze the oxidation of formate produced by glycolysis to carbon dioxide and molecular H2 (Gray and Gest, 1965). The overall reaction is scalar and functions to remove reducing equivalents exchangeable with formate and to help offset acidification of the growth medium. In the second pathway the bacteria are able to utilize H2 as an energy source in the presence of a nonfermentable carbon source, such as fumarate, which is acting as (or generating) a terminal electron acceptor (Macy et al., 1976). In this case, a respiratory hydrogenase catalyzes the oxidation (uptake) of H2 in an energy-conserving manner by proton translocation across the cytoplasmic membrane (Jones, 1980).
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© 1990 Plenum
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Mandrand, MA., Wu, LF., Boxer, D. (1990). Hydrogenase Mutants of Escherichia coli Defective in Nickel Uptake. In: Bélaich, JP., Bruschi, M., Garcia, JL. (eds) Microbiology and Biochemistry of Strict Anaerobes Involved in Interspecies Hydrogen Transfer. Federation of European Microbiological Societies Symposium Series, vol 54. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0613-9_30
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DOI: https://doi.org/10.1007/978-1-4613-0613-9_30
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