Abstract
In vivo high-resolution NMR with the Gram-negative Syntrophobacter wolinii and a Gram-positive syntrophic propionate oxidizing organism, indicated the involvement of an oxaloacetate: propionyl-CoA transcarboxylase in propionate oxidation. This finding was confirmed by enzyme measurement in cell-free extracts. Two sulphidogenic cocultures grew at a similar specific growth rate, whereas substitution of the hydrogenotrophic sulphate reducer by methane bacteria resulted in slower growth. The S. wolinii coculture had a lower cell yield than Desulfobulbus propionicus. This difference is explained in terms of energy conservation mechanisms.
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References
Ackman, R.G., 1972, Porous polymer bead packings and formic acid vapour in the GLC of volatile free fatty acids J. Chromatoqr. Sci., 10: 560
Boone, D.R. and Bryant, M.P., 1980, Propionate-degrading bacterium, Syntrophobacter wolinii sp. nov. gen., from methanogenic ecosystems, Appl. Environ. Microbiol., 40: 626.
Bradford, M.M., 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Analytical. Biochem., 72: 248
Bryant, M.P., 1979, J. Anim. Sci., 48: 193
Buswell, A.M. Fina, L. , Müller, H. and Yahiro, A., 1951, Use of 14C in mechanism studies of methane fermentation. II. propionic acid J. Am. Chem. Soc., 73: 1809.
Dolfing, J., 1988, Acetogenesis, in: Biology of Anaerobic Microorganisms, Zehnder, A.J.B., ed., Wiley, New York.
Hilpert, W. and Dimroth, P., 1982, Conversion of the chemical energy of methylmalonyl-CoA decarboxylation into a Na+ gradient, Nature, 296: 584.
Hilpert, W., Schink, B. and Dimroth, P., 1984, Life by a new decarboxylation-dependent energy conservation mechanism with Na+ as coupling ion, EMBO J., 3: 1665.
Houwen, F.P., Dijkema, C., Schoenmakers, C.H.H., Stams, A.J.M. and Zehnder, A.J.B., 1987, 13C-NMR study of propionate degradation by a methanogenic coculture, FEMS Microbiol. Letters, 41:269.
Houwen, F.P., Cheng Guangsheng, Folkers, G.E., Heuvel v. d., W.M.J.G. and Dijkema, C., 1988, Pyruvate and fumarate conversion by a methanogenic propionate-oxidizing coculture, in: Granular Anaerobic Sludge, Microbiology and Technology, Lettinga, G., Zehnder, A.J.B., Grotenhuis, J.T.C, and Hulshoff Pol, L.W., eds.,Pudoc, Wageningen.
Koch, M., Dolfing, J., Wuhrmann, K. and Zehnder, A.J.B., 1983, Pathways of propionate degradation by enriched methanogenic cultures, Appl. Environ. Microbiol., 45:1411
Konings, W.N ., 1985,Generation of metabolic energy by end-product efflux, TIBS - August 1985:317.
Kremer, D.R. and Hansen, T.A., 1988, Pathway of propionate degradation in Desulfobulbus propionicus, FEMS Microbiol.Lett., 49: 273.
Kuenen, J.G. and Veldkamp, H., 1972, Thiomicrospira pelophila gen. r. sp. n., a new obligately chemolithotroph colourless sulfur bacterium, Antonie van Leeuwenhoek, 38:241.
Laanbroek, H.J. and Pfennig, N., 1981, Oxidation of short-chain fatty acids by sulfate-reducing bacteria in freshwater and in marine sediments, Arch. Microbiol., 128:330.
McInerney, M.J., Bryant, M.P. and Pfennig, N.,1979, Anaerobic bacterium that degrades fatty acids in syntrophic association with methanogens, Arch. Microbiol., 122:129.
Mounfort, D.O. and Bryant, M.P., 1982, Isolation and characterization of an anaerobic syntrophic benzoate-degrading bacterium from sewage sludge, Arch. Microbiol., 133:249.
Pfennig, N. and Lippert, K.D., 1966, Ueber das Vitamin B12-Bedürfnis phototropher Schwefelbakterien, Arch. Microbiol., 55:245.
Robbins, J.E., 1988, A proposed pathway for catabolism of propionate in methanogenic cocultures, Appl. Environ. Microbiol., 54:1300.
Samain, E., Dubourguier, H.C. and Albagnac, G., 1984, Isolation and characterization of Desulfobulbus elongates sp. nov. from a mesophilic industrial digester, System. Appl. Microbiol., 5:391.
Schink, B.,1985, Mechanisms and kinetics of succinate and propionate degradation in anoxic freshwater sediments and sewage sludge, J.Gen. Microbiol., 131:643.
Schink, B., 1986, New aspects of fatty acid metabolism in anaerobic digestion, in: Proc. IV Int. Symp. Microbiol. Ecol. Ljubljana.
Skrabanja, A.T.P. and Stams, A.J.M., 1989, Oxidative propionate formation by anaerobic bacteria, in: This book.
Stams, A.J.M., Veenhuis, M., Weenk, G.H. and Hansen, T.A., 1983, Occurrence of polyglucose as a storage polymer in Desulfovibrio species and Desulfobulbus propionicus, Arch. Microbiol., 136:54.
Stams, A.J.M., Veenhuis, M., Weenk, G.H. and Hansen, T.A., 1983, Pathway of propionate formation in Desulfobulbus propionicus, Arch. Microbiol., 139:167.
Thauer, R.K., Jungermann, K. and Decker, K., 1977, Energy conservation in chemotrophic anaerobic bacteria, Bacteriol. Rev., 41:100.
Trüper, H.G. and Schlegel, H.G., 1964, Sulphur metabolism in Thiorodaceae. I. Quantitative measurements of growing cells of Chromatium okenii, Antonie van Leeuwenhoek J. Microbiol. Serol., 30: 225.
Widdel, F. and Pfennig, N., 1982, Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids II. Incomplete oxidation of propionate by Desulfobulbus propionicus gen. nov., sp. nov., Arch. Microbiol., 131: 360.
Widdel, F., 1988, Microbiology and ecology of sulfate- and sulfur-reducing bacteria, in: Biology of Anaerobic Microorganisms, Zehnder, A.J.B., ed., Wiley, New York.
Zehner, A.J.B., 1978, Ecology of methane formation, in: Water Pollution Microbiology, vol. 2, Mitchel, R., ed., Wiley, New York.
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© 1990 Plenum Press
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Houwen, F.P., Plokker, J., Dijkema, C., Stams, A.J.M. (1990). Syntrophic Propionate Oxidation. In: Bélaich, JP., Bruschi, M., Garcia, JL. (eds) Microbiology and Biochemistry of Strict Anaerobes Involved in Interspecies Hydrogen Transfer. Federation of European Microbiological Societies Symposium Series, vol 54. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0613-9_25
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DOI: https://doi.org/10.1007/978-1-4613-0613-9_25
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