Abstract
An anaerobic rod-shaped bacterium (strain K—1) was isolated from mesophilic digester sludge acclimatized with 4—"chlorobutyrate as main energy and carbon source. This bacterium was mesophilic, Gram-negative and motile, and degraded 4—chlorobutyrate to butyrate, acetate and hydrogen. Cell morphology varied from singles to pairs, and occasionally long chains. When growth peaked at a pH exceeding 6.0, cells immediately lysed, but not less than pH 6.0. The optimal conditions for growth were an initial pH 6.5–6.8 and 37°C. Growth required yeast extract or clarified rumen fluid, but neither peptone nor clarified sludge fluid could be replaced. Fructose was utilized as a carbon source, but not other sugars.
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© 1990 Plenum Press
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Matsuda, K., Nakamura, K., Kamagata, Y., Mikami, E. (1990). Isolation and Characterization of an Anaerobic Bacterium Degrading 4—Chlorobutyrate. In: Bélaich, JP., Bruschi, M., Garcia, JL. (eds) Microbiology and Biochemistry of Strict Anaerobes Involved in Interspecies Hydrogen Transfer. Federation of European Microbiological Societies Symposium Series, vol 54. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0613-9_18
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DOI: https://doi.org/10.1007/978-1-4613-0613-9_18
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