Lanthanide Chelates as Luminescent Probes

  • John L. Toner

Summary

A series of molecules is described which efficiently complex Eu+3 and Tb+3 and sensitize their luminescence in water. The general approach has been the incorporation of convergent binding sites on molecules which are efficient triplet sensitizers for lanthanides in aprotic solvents. One application for these chelators involves immunoluminescence assays in which a biologically relevant molecule present at low concentration could be quantitatively detected. Progress towards this goal is described.

Keywords

Manifold Amide Adduct Carbonyl Halflife 

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References

  1. 1.
    a) Hinshaw, J. C.; Toner, J. L.; Reynolds, G. A. Fluorescent Labels for Immunoassays, U. S. Patent No. 4,637,988, 1987; (b) Hinshaw, J. C.; Toner, J. L.; Reynolds, G. A. Phenolic Fluorescent Labels, U. S. Patent No. 4,637, 988, 1987.Google Scholar
  2. 2.
    Soini, E.; Hemmila, I. Clin. Chem. 1979, 25, 353 - 361.Google Scholar
  3. 3.
    Soini, E. In Rapid Method Autom. Microbiol. Immunol., 4th Int. Symp.; Habermehl, K.-O., Ed.; Springer: Berlin, 1985; pp 414–421.Google Scholar
  4. 4.
    Sinha, A. P. B. Spectroscop. Inorg. Chem. 1971, 2, 255–288.Google Scholar
  5. 5.
    Alpha, B.; Balzani, V.; Lehn, J.-M.; Perathoner, S.; Sabbatini, N. Angew. Chem., Int. Ed. Engl. 1987, 26, 1266–1267.CrossRefGoogle Scholar
  6. 6.
    Horrocks, W. DeW., Jr.; Sudnick, D. R. Acc. Chem. Res. 1981, 14, 383–392.CrossRefGoogle Scholar
  7. 7.
    a) Soini, K.; Kojola, H. Clin. Chem. 1983,29, 65–68; (b) Hemmila, D. S.; Mukkala, V.-M.; Siitari, H.; Lovgren, T. Anal. Biochem. 1984,137, 335–343; (c) Lovgren, T.; Hemmila, I.; Pettersson, K.; Eskola, J. U.; Bertoft, E. Talanta 1984, 31, 909–919.Google Scholar
  8. 8.
    a) Blasse, G.; Dirksen, G. J.; Sabbatini, N.; Perathoner, S.; Lehn, J.-M.; Alpha,B. J. Chem. Phys. 1988, 92, 2419–2422; (b) Blasse, G.; Dirksen, G. J.; Van Der Voort, D.; Sabbatini, N.; Perathoner, S.; Lehn, J.-M.; Alpha, B. Chem. Phys. Lett. 1988,146, 347–351; (c) Alpha, B.; Lehn, J.-M.; Mathis. G. Angew. Chem., Int. Ed. Engl. 1987, 26, 266–267; (d) Sabbatini, N.; Perathoner, S.; Balzani, V.; Alpha, B.; Lehn, J.-M. NATO ASI Ser., Ser. C, (Supramol. Photochem.) 1987,274, 187–206; (e) Lehn, J.-M. NATO ASI Ser., Ser. C, (Supramol. Photochem.) 1987, 241, 29–43.Google Scholar
  9. 9.
    Specht, D. P.; Martic, P. A.; Farid, S. Tetrahedron 1982, 38, 1203–1211.CrossRefGoogle Scholar
  10. 10.
    Wheelwright, E. J.; Spedding, F. H.; Schwarzenbach, G. J. Am. Chem. Soc. 1953, 75, 4196–4201.CrossRefGoogle Scholar
  11. 11.
    Vögtle, F.; Ohm, C. Chem. Ber. 1984, 117, 948–954.CrossRefGoogle Scholar
  12. 12.
    Ohm, C.; Vögtle, F. Chem. Ber. 1985, 118, 22–27.CrossRefGoogle Scholar
  13. 13.
    Chandler, C. J.; Deady, L. W.; Reiss, J. A. J. Heterocyclic Chem. 1981, 18, 599–601.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • John L. Toner
    • 1
  1. 1.Exploratory Sciences Division Life Sciences Research LaboratoriesEastman Kodak CompanyRochesterUSA

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