Nucleic Acid Hybridization as a Diagnostic Tool for the Detection of Human Papillomaviruses

  • Robert F. Rando
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 263)

Abstract

Human papillomavirus (HPV) infections of the anogenital tract present several difficult questions pertaining to diagnosis. The most obvious question is whether a test for papillomavirus in anogenital lesions is necessary, and if so, what information is desired from such a test. If a diagnostic test is sought, then the next problem concerns the multitude of different papillomavirus types found in anogenital tract lesions, the need, if any, to differentiate these multiple types, and the availability of diagnostic probes to do so. The following discussion will elucidate the arguments supporting the need to test for HPV, by briefly describing a number of epidemiologic studies which associate HPV DNA with premalignant and malignant anogenital tract lesions. Molecular biological data which demonstrates the ability of certain papillomaviruses to transform cells in culture will also be reviewed. In addition, the ways in which nucleic acid hybridization techniques apply to the detection of HPV infections will be described, as well as advantages and disadvantages of several different hybridization techniques which can be used to HPV detection in anogenital lesions.

Keywords

Electrophoresis Nylon Adenine Pyrimidine Biotin 

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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Robert F. Rando
    • 1
  1. 1.Departments of Pathology and OB/GYNPennsylvania HospitalPhiladelphiaUSA

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