Abstract
A cDNA library from Chlamydomonas reinhardtii was probed with antiserum directed against the nuclear encoded γ subunit of the chloroplast H+-translocating ATP synthase (CF0-CF1) of C. reinhardtii. A cDNA was isolated and transcribed in vitro. The transcript was translated in vitro and immunoprecipitated with anti- γ serum to yield a product that co-electrophoresed with the immunoprecipitated product from in vitro translated polyadenylated RNA. These proteins were larger than the mature γ subunit. Thus the γ subunit is synthesized as a precursor of greater molecular weight in C. reinhardtii, as has been suggested for the y subunit from spinach (Nelson et al., 1980).
The precursor protein encoded by this cDNA was imported into pea chloroplasts and processed to a lower molecular weight polypeptide that co-electrophoresed with mature C. reinhardtii γ subunit. The largest cDNA isolated is about the same length as the corresponding mRNA (about 2000 bases). Southern analyses revealed restriction fragment length polymorphisms and that the γ subunit is probably encoded by an intron-containing single copy gene.
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© 1989 Plenum Press, New York
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Yu, L.M., Theg, S.M., Selman, B.R. (1989). Isolation of a cDNA Clone for the γ Subunit of the Chlamydomonas Reinhardtii CF1 . In: Marzuki, S. (eds) Molecular Structure, Function, and Assembly of the ATP Synthases. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0593-4_9
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DOI: https://doi.org/10.1007/978-1-4613-0593-4_9
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