Abstract
The ATP photoaffinity probe arylazido-β-alanyl ATP is able to inhibit the ATP dependent reactions of the Rhodospirillum rubrum chromatophore membrane in a specific and light dependent manner. Following the light induced photoprobe interaction with LiCl treated chromatophores (resolved of the ATPase β subunit), the LiCl dialyzed supernatant (i.e., β subunit) is still capable of reconstituting photophosphorylation as in the non-photoprobe treated membrane. However, the ability of the β subunit preparation to reconstitute membrane bound ATPase activity is inhibited.
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Abbreviations
- arylazido-β-alanyl ATP:
-
A3’-O-3-[N-(4-azido-2-nitrophenyl)amino]propionyl ATP
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© 1989 Plenum Press, New York
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Guillory, R.J. (1989). Interaction of Arylazido-β-Alanyl ATPa with the ATPase Enzyme of Rhodospirillum Rubrum Chromatophores. In: Marzuki, S. (eds) Molecular Structure, Function, and Assembly of the ATP Synthases. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0593-4_23
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DOI: https://doi.org/10.1007/978-1-4613-0593-4_23
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-7882-5
Online ISBN: 978-1-4613-0593-4
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