Parathyroid Hormone Stimulates Colony Formation of Chick Embryo Chondrocytes in Soft Agar
Chondrocytes isolated from 16- to 19-day-old chick embryos were seeded and maintained for 3 weeks in soft agar. No colony formation was observed without added hormones or growth factors even in the presence of 10% serum. PTH induced colony formation by the cells. This effect was detected at PTH concentrations as low as 10-10 M. The maximal stimulation of colony formation was observed at 10-8 M. The colony-forming efficiency of PTH-maintained cultures was 6-9%. Dibutyryl cyclic-AMP (0. 1 mM) also stimulated soft agar growth of chick embryo chondrocytes. However, Prostaglandin 12 (30 μM) did not stimulate colony formation, although it increased cyclic-AMP levels in chondrocytes. Thus, the growth-promoting effect of PTH is not mediated by cyclic-AMP. PTH had lesser effect on soft agar growth of chondrocytes that were isolated from sternal cartilages of postnatal 14-day-old chickens and rib cartilages of 28-day-old rabbits.
KeywordsAgar Agarose Polysaccharide Adenosine Trypsin
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- Burch, W. M. and Lebovitz, H. E. 1983, Parathyroid hormone stimulates growth of embryonic chick pelvic cartilage in vitro. Calcif. Tissue Int., 35:526.Google Scholar
- Guri, C. D. and Bernstein, D. S., 1964. Effect of parathyroid hormone on mucopolysaccharide synthesis in rachitic rat cartilage in vitro Proc. Soc. Exp. Biol. Med., 116:702.Google Scholar
- Kawashima, K., Iwata, S., and Endo, H., 1980. Growth stimulate effect of parathyroid hormone, calcitonin and N6, O2’ -dibutyryl adenosine 3’ ;5’ -cyclic monophosphoric acid on chick embryonic cartilage cultivated in a chemically defined medium. Endocrinol. Japon., 27:349.Google Scholar
- Lewinson, D. and Silbermann, M., 1986. Parathyroid hormone stimulates proliferation of chondroprogenitor cells in vitro. Calcif. Tissue Int., 38:155.Google Scholar
- Suzuki, F., Yoneda, T., Shimomura, Y., 1976, Calcitonin and parathyroid-hormone stimulation of acid mucopolysaccharide synthesis in cultured chondrocytes isolated from growth cartilage, FEBS Lett., 70: 155.Google Scholar
- Takano, T., Takigawa, M., Shirai, E., Suzuki, F., Rosenblatt, M., 1985, Effects of synthetic analogs and fragments of bovine parathyroid hormone on adenosine 3’,5’ -monophosphate level, ornithine decarboxylase activity, and glycosaminoglycan synthesis in rabbit costal chondrocytes in culture: Structure-activity relations, Endocrinology, 116: 2536.PubMedCrossRefGoogle Scholar
- Takigawa, M., Isida, H., Takano, T., and Suzuki, F., 1980, Polyamine and differentiation: Induction of ornithine decarboxylase by parathyroid hormone is good marker of differentiated chondrocytes. Proc. Natl. Sci. U. S. A., 77:1481.Google Scholar
- Takigawa, M., Takano, T., Suzuki, F., 1981, Effects of parathyroid hormone and cyclic AMP analogues on the activity of ornithine decarboxylase and expression of the differentiated phenotype of chondrocytes in culture. J. Cell. Physiol., 106:259Google Scholar
- Zull, J. E., Youngman, K., and Caplan, A. I., 1981, The development of hormonal responses of cultured embryonic chick limb mesenchymal cells. Develop. Biol., 86:61.Google Scholar