Parathyroid Hormone Stimulates Colony Formation of Chick Embryo Chondrocytes in Soft Agar

  • Tatsuya Koike
  • Yukio Kato
  • Masahiro Iwamoto
  • Fujio Suzuki

Summary

Chondrocytes isolated from 16- to 19-day-old chick embryos were seeded and maintained for 3 weeks in soft agar. No colony formation was observed without added hormones or growth factors even in the presence of 10% serum. PTH induced colony formation by the cells. This effect was detected at PTH concentrations as low as 10-10 M. The maximal stimulation of colony formation was observed at 10-8 M. The colony-forming efficiency of PTH-maintained cultures was 6-9%. Dibutyryl cyclic-AMP (0. 1 mM) also stimulated soft agar growth of chick embryo chondrocytes. However, Prostaglandin 12 (30 μM) did not stimulate colony formation, although it increased cyclic-AMP levels in chondrocytes. Thus, the growth-promoting effect of PTH is not mediated by cyclic-AMP. PTH had lesser effect on soft agar growth of chondrocytes that were isolated from sternal cartilages of postnatal 14-day-old chickens and rib cartilages of 28-day-old rabbits.

Keywords

Agar Agarose Polysaccharide Adenosine Trypsin 

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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Tatsuya Koike
    • 1
  • Yukio Kato
    • 1
  • Masahiro Iwamoto
    • 1
  • Fujio Suzuki
    • 1
  1. 1.Department of BiochemistryOsaka University, Faculty of DentistryOsakaJapan

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