Summary
Chondrocytes isolated from 16- to 19-day-old chick embryos were seeded and maintained for 3 weeks in soft agar. No colony formation was observed without added hormones or growth factors even in the presence of 10% serum. PTH induced colony formation by the cells. This effect was detected at PTH concentrations as low as 10-10 M. The maximal stimulation of colony formation was observed at 10-8 M. The colony-forming efficiency of PTH-maintained cultures was 6-9%. Dibutyryl cyclic-AMP (0. 1 mM) also stimulated soft agar growth of chick embryo chondrocytes. However, Prostaglandin 12 (30 μM) did not stimulate colony formation, although it increased cyclic-AMP levels in chondrocytes. Thus, the growth-promoting effect of PTH is not mediated by cyclic-AMP. PTH had lesser effect on soft agar growth of chondrocytes that were isolated from sternal cartilages of postnatal 14-day-old chickens and rib cartilages of 28-day-old rabbits.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Benya, P. D., Shaffer, J. D., 1982, Dedifferentiated chondrocytes re-express the differentiated collagen phenotype when cultured in agarose gels, Cell, 30: 215.
Burch, W. M. and Lebovitz, H. E. 1983, Parathyroid hormone stimulates growth of embryonic chick pelvic cartilage in vitro. Calcif. Tissue Int., 35:526.
Guri, C. D. and Bernstein, D. S., 1964. Effect of parathyroid hormone on mucopolysaccharide synthesis in rachitic rat cartilage in vitro Proc. Soc. Exp. Biol. Med., 116:702.
Kawashima, K., Iwata, S., and Endo, H., 1980. Growth stimulate effect of parathyroid hormone, calcitonin and N6, O2’ -dibutyryl adenosine 3’ ;5’ -cyclic monophosphoric acid on chick embryonic cartilage cultivated in a chemically defined medium. Endocrinol. Japon., 27:349.
Lewinson, D. and Silbermann, M., 1986. Parathyroid hormone stimulates proliferation of chondroprogenitor cells in vitro. Calcif. Tissue Int., 38:155.
Suzuki, F., Yoneda, T., Shimomura, Y., 1976, Calcitonin and parathyroid-hormone stimulation of acid mucopolysaccharide synthesis in cultured chondrocytes isolated from growth cartilage, FEBS Lett., 70: 155.
Takano, T., Takigawa, M., Shirai, E., Suzuki, F., Rosenblatt, M., 1985, Effects of synthetic analogs and fragments of bovine parathyroid hormone on adenosine 3’,5’ -monophosphate level, ornithine decarboxylase activity, and glycosaminoglycan synthesis in rabbit costal chondrocytes in culture: Structure-activity relations, Endocrinology, 116: 2536.
Takigawa, M., Isida, H., Takano, T., and Suzuki, F., 1980, Polyamine and differentiation: Induction of ornithine decarboxylase by parathyroid hormone is good marker of differentiated chondrocytes. Proc. Natl. Sci. U. S. A., 77:1481.
Takigawa, M., Takano, T., Suzuki, F., 1981, Effects of parathyroid hormone and cyclic AMP analogues on the activity of ornithine decarboxylase and expression of the differentiated phenotype of chondrocytes in culture. J. Cell. Physiol., 106:259
Zull, J. E., Youngman, K., and Caplan, A. I., 1981, The development of hormonal responses of cultured embryonic chick limb mesenchymal cells. Develop. Biol., 86:61.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Plenum Press, New York
About this chapter
Cite this chapter
Koike, T., Kato, Y., Iwamoto, M., Suzuki, F. (1989). Parathyroid Hormone Stimulates Colony Formation of Chick Embryo Chondrocytes in Soft Agar. In: Massry, S.G., Fujita, T. (eds) New Actions of Parathyroid Hormone. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0567-5_49
Download citation
DOI: https://doi.org/10.1007/978-1-4613-0567-5_49
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-7870-2
Online ISBN: 978-1-4613-0567-5
eBook Packages: Springer Book Archive