Parathyroid Hormone Receptors in Cultured Rat Vascular Smooth Muscle and Bovine Endothelial Cells

  • Yukio Hirata
  • Yasuyuki Takagi
  • Takuo Fujita

Abstract

It has been reported that parathyroid hormone (PTH) causes a marked decrease in blood pressure in a variety of species1,2 and relaxes pre-contracted vascular strips from conduit- to resistance-type vessels 3,4,5 . Therefore, PTH may have direct action on the vascular tissues other than kidney and bone. However, little is known about the cellular mechanism of vascular action by PTH. To elucidate the mechanism of PTH action in the vasculature, we have attempted to identify and characterize vascular receptors for PTH in cultured rat vascular smooth muscle cells (VSMC) and bovine endothelial cells (EC) using 125I-labeled-synthetic amino-terminal fragment of human (h) PTH as radioligand.

Keywords

Rubber Fluoride Adenosine Angiotensin Prostaglandin 

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References

  1. 1.
    P.K.T. Pang, T.E. Tenner, Jr., J.A. Yee, M. Yang, H.F. Janssen, Hypotensive action of parathyroid hormone preparations on rats and dogs,Proc Natl Acad Sci USA 77: 675 (1980).Google Scholar
  2. 2.
    H.H. Wang, E.D. Drugge, Y.C. Yen, M.R. Blumenthal, P.K.T. Pang, Effects of synthetic parathyroid hormone on hemodynamics and regional blood flows, Eur J Pharmacol 97: 209 (1984).PubMedCrossRefGoogle Scholar
  3. 3.
    P.K.T. Pang, M.C.M. Yang, R. Shew, T.E. Tenner, Jr., The vosorelaxant action of parathyroid hormone fragments on isolated rat tail artery, Blood Vessels 22: 57 (1985).PubMedGoogle Scholar
  4. 4.
    G.A. Nikols, M.A. Metz, and W.H. Chine, Jr., Vasodilation of the rat mesenteric vasculature by parathyroid hormone, J. Pharmacol Exp Ther 236: 419 (1986).Google Scholar
  5. 5.
    Y. Suzuki, K. Lederis, M. Huang, F.E. LeBlanc, O.P. Rostad, Relaxation of bovine, porcine and human brain arteries by parathy-roid hormone, Life Sci 33: 2497 (1983).PubMedCrossRefGoogle Scholar
  6. 6.
    Y. Hirata, M. Tornita, H. Yoshimi, M. Ikeda, Specific receptors for atrial natriuretic factor (ANF) in cultured vascular smooth muscle cells of rat aorta, Biochem Biophys Res Commun 125: 562 (1984).PubMedCrossRefGoogle Scholar
  7. 7.
    Y. Hirata, M. Tomita, S. Takata, T. Fujita, Functional receptors for vasoactive intestinal peptide in cultured vascular smooth muscle cells from rat aorta, Biochem Biophys Res Commun 132: 1079 (1985).PubMedCrossRefGoogle Scholar
  8. 8.
    Y. Hirata, M. Tomita, S. Takata, I. Inoue, Specific binding of atrial natriuretic peptide (ANP) in cultured mesenchymal non-myocardial cells from rat heart, Biochem Biophys Res Commun 131: 222 (1985).PubMedCrossRefGoogle Scholar
  9. 9.
    M. Shinjo, Y. Hirata, H. Hagiwara, F. Akiyama, K. Murakami, S. Kojima, M. Shimonaka, Y. Inada, S. Hirose, Characterization of atrial natriuretic factor receptors in adrenal cortex, vascular smooth muscle and endothelial cells by affinity labeling, Biomed Res 7: 35 (1986).Google Scholar
  10. 10.
    G.A. Nickols, M.A. Metz, W.H. Cline, Jr., Endothelium-independent linkage of parathyroid hormone receptors of rat vascular tissues with increased adenosine 3f,5f-monophosphate and relaxation of vascular smooth muscle, Endocrinology 119: 349 (1986).PubMedCrossRefGoogle Scholar
  11. 11.
    M.D. Coltrera, J.T. Potts, Jr., M. Rosenblatt, Identification of a renal receptor for parathyroid hormone by photoaffinity radio-labeling using a synthetic analogue, J Biol Chem 256: 10555 (1981).PubMedGoogle Scholar
  12. 12.
    M.W. Draper, R.A. Nissenson, J. Winer, J. Ramachandran, CD. Arnaud, Photoaffinity labeling of the canine renal receptor for parathyroid hormone, J Biol Chem 257: 3714 (1982).PubMedGoogle Scholar
  13. 13.
    B.S. Wright, G.A. Tyler, R. O’Brien, L.H. Caporale, M. Rosenblatt, Immunoprecipitation of the parathyroid hormone receptor, Proc Natl Acad Sci USA 84: 26 (1987).PubMedCrossRefGoogle Scholar
  14. 14.
    R.A. Nissenson, D. Karpf, T. Bambino, J. Winner, M. Canga, K. Nyiredy, CD. Arnaud, Covalent labeling of a high-affinity, guanyl nucleotide sensitive parathyroid hormone receptor in canine renal cortex, Biochemistry 26: 1874, 1987.PubMedCrossRefGoogle Scholar
  15. 15.
    J. Chuang, I. Yike, J.H. Reese, R. Laethem, J.E. Zull, Identification and purification of a kidney membrane protein which specifically binds the amino-terminal domain of native parathyroid hormone, J Biol Chem 262: 10760 (1987).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Yukio Hirata
    • 1
    • 2
  • Yasuyuki Takagi
    • 1
    • 2
  • Takuo Fujita
    • 2
  1. 1.Hypertension DivisionNational Cardiovascular Cener Research InstituteOsakaJapan
  2. 2.Department of MedicineKobe University School of MedicineKobeJapan

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