Skip to main content

Discrimination and Thresholding

  • Chapter
Computer-Assisted Microscopy

Abstract

There have been several references in the preceding chapters to the use of brightness discrimination to select pixels belonging to features of interest. This is a widely used method of converting a grey scale image to a binary (black and white) one, illustrated in Figure 5-1. Discrimination with threshold values is much more efficient than any edge following or region growing method (as discussed in the previous chapter) because it works on the entire image at once. Hence the time required is fixed, regardless of the complexity of the image, and very short. Also, the resulting binary image is a pixel-based representation of features of interest, and is easier for most measurement operations than the boundary representation that results from the location and identification of edges.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • D. S. Bright, E. B. Steel (1986) Bright-field image correction with various imageprocessing tools Microbeam Analysis 1986 (A.D. Romig, Jr., W.F. Chambers, eds.) San Francisco Press, 517-520

    Google Scholar 

  • D. S. Bright (1987) An object finder based on multiple thresholds, connectivity and internal structure Microbeam Analysis 1987 (R.H. Geiss, ed.) San Francisco Press 1987, 290-292

    Google Scholar 

  • K. R. Castleman (1979) Digital Image Processing, Prentice Hall, Englewood Cliffs, NJ

    Google Scholar 

  • K. R. Castleman, J. Melnyk (1976) An Automated System for Chromosome Analysis: Final Report, Document 5040-30, Jet Propulsion Lab., Pasadena, CA

    Google Scholar 

  • R. M. Haralick, K. Shanmugam, I. Dinstein (1913) Textural Features for Image Classification IEEE Trans. Syst. Man. Cybern., SMC- 3 610–621

    Article  Google Scholar 

  • J. N. Kanpur, P. K. Sahoo, A. K. C. Wong (1985) A new method for grey-level picture thresholding using the entropy of the histogram CVGIP 29, 273–285

    Google Scholar 

  • J. G. Moik (1980) Digital Processing of Remotely Sensed Images NASA publication SP - 431, 277

    Google Scholar 

  • J. F. O’Callaghan (1974) Computing the Perceptual Boundaries of Dot Patterns Computer Graphics and Image Processing 3 # 2,141–162

    Article  Google Scholar 

  • T. Pavlidis (1982) Algorithms for Graphics and Image Processing, Computer Science Press, Rockville MD

    Google Scholar 

  • W. K. Pratt (1978) Digital Image Processing Wiley, New York

    Google Scholar 

  • J. Prewitt, M. Mendelsohn (1966) The Analysis of Cell Images Annals of the N.Y. Academy of Sciences 128,1035–1053

    Article  CAS  Google Scholar 

  • J. P. Rigaut (1988) Automated image segmentation by mathematical morphology and fractal geometry Journal of Microscopy 150 21–30

    Article  Google Scholar 

  • A. Rosenfeld (1979) Some experiments on variable thresholding Pattern Recognition 11, 191

    Article  Google Scholar 

  • A. Rosenfeld, A. C. Kak (1982) Digital Picture Processing Academic Press, London

    Google Scholar 

  • J. C. Russ, J. Ch. Russ (1984) Image processing in a general purpose microcomputer J. Microscopy 135, 89

    Article  Google Scholar 

  • J. C. Russ (1986) Practical Stereology Plenum Press, New York

    Google Scholar 

  • J. C. Russ, J. Ch. Russ (1988) Automatic discrimination of features in grey-scale images Journal of Microscopy 148 263–277

    Article  Google Scholar 

  • A. W. M. Smeulders, A. D. Beckers (1989) Accurate image measurement methods (applied to 3D length and distance measurements) Proc. 1st International Conf. on Confocal Microscopy, Academisch Medisch Centrum, Amsterdam

    Google Scholar 

  • R. J. Wall, A. Klinger, K. R. Castleman (1974) Analysis of Image Histograms, Proc 2nd Joint Int’l Conference on Patt. Recog., IEEE 74CH-0885-4C. 341-344

    Google Scholar 

  • J. Weszka (1978) A Survey of Threshold Selection Techniques Comp. Graph. & Image Proc. 7, 259–265

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

Copyright information

© 1990 Plenum Press, New York

About this chapter

Cite this chapter

Russ, J.C. (1990). Discrimination and Thresholding. In: Computer-Assisted Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0563-7_5

Download citation

  • DOI: https://doi.org/10.1007/978-1-4613-0563-7_5

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-7868-9

  • Online ISBN: 978-1-4613-0563-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics