Use of Promoter Modified Electrodes for Heme Protein Electrochemistry
In recent years, electrochemical studies of proteins such as cytochrome c have become possible by conventional electrochemical techniques such as cyclic voltammetry by using several types of electrodes: For example, promoter modified electrodes, of which surfaces are modified by suitable compounds, so-called promoters, for the rapid electron transfer of proteins, have been developed by the author’s group (Taniguchi et al, 1982; Taniguchi et al., 1986b; Taniguchi, 1988); some metal oxides such as indium oxide are also useful (Yeh and Kuwana, 1977; Bowden et al, 1982; Harmer and Hill, 1984); also, in some cases, bare metal electrodes without any surface modification are reported to be used (Bowden et al, 1984; Reed and Hawkridge, 1987; Sun et al., 1988). Among these electrodes, promoter modified electrodes are sometimes more convenient to use than other electrodes, because they can be prepared so easily (e.g., just dipping an electrode into a promoter solution), show very reproducible results, and are applicable under various experimental conditions, when we choose a suitable promoter.
KeywordsSurface Enhance Raman Scattering Circular Dichroism Spectrum Gold Electrode Electron Transfer Reaction Indium Oxide
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- Bowden, E. F., Hawkridge, F.M., Chlebowski, J.F., Bancroft, E.E., Thorpe, C., and Blount, H.N., 1982, Cyclic Voltarametry and Derivative Cyclic Voltabsorptometry of Purified Horse Heart Cytochrome c at Tin-Doped Indium Oxide Optically Transparent Electrodes, J. Am. Chem. Soc., 104:7461.CrossRefGoogle Scholar
- Heineman, W.R., Hawkridge, F.M., and Blount, H.N., 1984, Spectroelectrochemistry at Optically Transparent Electrodes. II. Electrodes Under Thin- Layer and Semi-infinite Diffusion Conditions and Indirect Coulometric Titrations, in: “Electroanalytical Chemistry,” Vol. 13, A.J. Bard, ed., Marcel Dekker, New York, 1.Google Scholar
- Ikeshoji, T. Taniguchi, I., and Hawkridge, F.M., 1989, Electrochemically Distinguishable States of Ferricytochrome c and Their Transition with Changes in Temperature and pH, J. Electroanal. Chem., in press.Google Scholar
- Taniguchi, I., Toyosawa, K., Yamaguchi, H., and Yasukouchi, K., 1982, Reversible Electrochemical Reduction and Oxidation of Cytochrome c at a Bis(4- pyridyl)disulfide Modified Gold Electrode, J. Chem. Soc. Chem. Commun., 1032; Voltammetric Response of Horse Heart Cytochrome c at a Gold Electrode in the Presence of Sulfur Bridged Bipyridines, J. Electroanal. Chem., 140:187.Google Scholar
- Taniguchi, I., Iseki, M., Yamaguchi, H., and Yasukouchi, K., 1985, Surface Enhanced Raman Scattering from Bis(4-pyridyl)disulfide and 4,4′- Bipyridine Modified Gold Electrodes, J. Electroanal. Chem., 186:299.Google Scholar
- Taniguchi, I., 1987, Promoter Modified Electrodes as Probes for Analysis of the Interaction Between Cytochrome c and Cytochrome c Oxidase,in: “Proceedings of the 2nd Japan-Korea Joint Symposium on Analytical Chemistry,” Y. Ohkura ed., Society of Analytical Chemistry, Japan, Tokyo, 2: 55.Google Scholar
- Taniguchi, I., 1988, Interfacial Electrochemistry of Promoter Modified Electrodes for the Rapid Electron-Transfer of Cytochrome c, in:“Redox Chemistry and Interfacial Behavior of Biological Molecules,” G. Dryhurst and K. Niki eds., Plenum, New York, 113.Google Scholar
- Yeh, P. and Kuwana, T., 1977, Reversible Electrode Reaction of Cytochrome c, Chem. Lett., 1145.Google Scholar