Abstract
Enzyme-dependent and non-enzymatic in vitro lipid peroxidation was studied in autoimmune prone B/W mice fed diets containing high levels of dietary corn oil (CO) or menhaden fish oil (FO) as lipid source since weaning. Lipid analysis revealed that FO-fed mouse liver mitochondrial and microsomal membrane fractions incorporated 20:5ω3 and 22:6ω3 in replacement of 18:2ω6 and 20:4ω6 found in corn oil (CO) fed control animals reflecting the composition of the dietary oils. Lower concentrations of vitamin E were found in the FO-fed mouse membranes and serum than those of CO-fed mice when diets were supplemented with a standard 75 I.U. α-tocopheryl acetate/kg diet. The rate and extent of membrane lipid peroxidation was greatly increased in FO-fed, vitamin-E-depleted membranes. Full repletion of membrane vitamin E levels by supplementation with 500 I.U./kg of FO diet for 30 days significantly decreased lipid peroxidation and showed that in FO-fed mice, membrane peroxidation is inversely proportional to vitamin E content. However, due to a lower ratio of vitamin E and highly unsaturated fatty acids, FO-fed mouse membranes were more sensitive to pro-oxidant stimulus than were those from CO-fed mice. These findings illustrate the action of vitamin E against membrane lipid peroxidation and stress the importance of adequate supplementation of antioxidant with high omega-3 fatty acids intake.
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© 1990 Plenum Press, New York
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Laganiere, S., Yu, B.P., Fernandes, G. (1990). Studies on Membrane Lipid Peroxidation in Omega-3 Fatty Acid-Fed Autoimmune Mice: Effect of Vitamin E Supplementation. In: Bendich, A., Phillips, M., Tengerdy, R.P. (eds) Antioxidant Nutrients and Immune Functions. Advances in Experimental Medicine and Biology, vol 262. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0553-8_8
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DOI: https://doi.org/10.1007/978-1-4613-0553-8_8
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