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Studies on Membrane Lipid Peroxidation in Omega-3 Fatty Acid-Fed Autoimmune Mice: Effect of Vitamin E Supplementation

  • Serge Laganiere
  • Byung P. Yu
  • Gabriel Fernandes
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 262)

Abstract

Enzyme-dependent and non-enzymatic in vitro lipid peroxidation was studied in autoimmune prone B/W mice fed diets containing high levels of dietary corn oil (CO) or menhaden fish oil (FO) as lipid source since weaning. Lipid analysis revealed that FO-fed mouse liver mitochondrial and microsomal membrane fractions incorporated 20:5ω3 and 22:6ω3 in replacement of 18:2ω6 and 20:4ω6 found in corn oil (CO) fed control animals reflecting the composition of the dietary oils. Lower concentrations of vitamin E were found in the FO-fed mouse membranes and serum than those of CO-fed mice when diets were supplemented with a standard 75 I.U. α-tocopheryl acetate/kg diet. The rate and extent of membrane lipid peroxidation was greatly increased in FO-fed, vitamin-E-depleted membranes. Full repletion of membrane vitamin E levels by supplementation with 500 I.U./kg of FO diet for 30 days significantly decreased lipid peroxidation and showed that in FO-fed mice, membrane peroxidation is inversely proportional to vitamin E content. However, due to a lower ratio of vitamin E and highly unsaturated fatty acids, FO-fed mouse membranes were more sensitive to pro-oxidant stimulus than were those from CO-fed mice. These findings illustrate the action of vitamin E against membrane lipid peroxidation and stress the importance of adequate supplementation of antioxidant with high omega-3 fatty acids intake.

Keywords

Membrane Fraction Microsomal Membrane Fraction Full Repletion Polyunsaturated Fatty Acid Eicosapentaenoic Acid Fatty Acid Analysis Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Serge Laganiere
    • 1
  • Byung P. Yu
    • 1
  • Gabriel Fernandes
    • 1
  1. 1.Department of Medicine and PhysiologyThe University of Texas Health Science Center at San AntonioUSA

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