Studies on Membrane Lipid Peroxidation in Omega-3 Fatty Acid-Fed Autoimmune Mice: Effect of Vitamin E Supplementation

  • Serge Laganiere
  • Byung P. Yu
  • Gabriel Fernandes
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 262)


Enzyme-dependent and non-enzymatic in vitro lipid peroxidation was studied in autoimmune prone B/W mice fed diets containing high levels of dietary corn oil (CO) or menhaden fish oil (FO) as lipid source since weaning. Lipid analysis revealed that FO-fed mouse liver mitochondrial and microsomal membrane fractions incorporated 20:5ω3 and 22:6ω3 in replacement of 18:2ω6 and 20:4ω6 found in corn oil (CO) fed control animals reflecting the composition of the dietary oils. Lower concentrations of vitamin E were found in the FO-fed mouse membranes and serum than those of CO-fed mice when diets were supplemented with a standard 75 I.U. α-tocopheryl acetate/kg diet. The rate and extent of membrane lipid peroxidation was greatly increased in FO-fed, vitamin-E-depleted membranes. Full repletion of membrane vitamin E levels by supplementation with 500 I.U./kg of FO diet for 30 days significantly decreased lipid peroxidation and showed that in FO-fed mice, membrane peroxidation is inversely proportional to vitamin E content. However, due to a lower ratio of vitamin E and highly unsaturated fatty acids, FO-fed mouse membranes were more sensitive to pro-oxidant stimulus than were those from CO-fed mice. These findings illustrate the action of vitamin E against membrane lipid peroxidation and stress the importance of adequate supplementation of antioxidant with high omega-3 fatty acids intake.


Membrane Fraction Microsomal Membrane Fraction Full Repletion Polyunsaturated Fatty Acid Eicosapentaenoic Acid Fatty Acid Analysis Membrane 
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  1. Bang, H. O., and Dyerberg, J., 1980, Plasma lipids and lipoprotein pattern in Greenlandic west coast Eskimos, Lancet, i:1143.Google Scholar
  2. Burk, R., 1985, Glutathione-dependent protection by rat liver microsomal protein against lipid peroxidation, Biochim. Biophys. Acta., 757:21.Google Scholar
  3. Cheeseman, K. H., Collins, M., Proudfoot, K., Slater, T. F., Burton, G. W., Webb, A. C., and Ingold, K. U., 1986, Studies on lipid peroxidation in normal and tumor tissues. The Novikoff rat liver tumor, Biochem. J., 253:507.Google Scholar
  4. Dillard, C. J., Litov, R. E., and Tappel, A. L., 1978, Effects of dietary vitamin E, selenium, and polyunsaturated fats on in vivo lipid peroxidation in the rat as measured by pentane production, Lipids, 13:396.CrossRefGoogle Scholar
  5. Farrel, P. M., and Bieri, J. G., 1975, Megavitamin E supplementation in man, Am. J. Clin. Nutr., 28:1381.Google Scholar
  6. Fernandes, G., 1987, Influence of nutrition on autoimmune disease, in:“Aging and the Immune Response,” E. J. Goidl, ed., Marcel Dekker, Inc., New York.Google Scholar
  7. Fernandes, G., Gaunt, C., Sandberg, L., Friedrichs, W. E., and Meydani, S. N., 1987, Effect of high fish oil intake on vitamin E levels, NK activity and susceptibility to viral-induced myocarditis in lupus-prone mice, Arth. Rheum., 30:S123.Google Scholar
  8. Folch, J., Lees, M., and Sloane-Stanley, G. H., 1957, A simple method for the isolation and purification of total lipids from animal tissues, J. Biol. Chem., 226:497.Google Scholar
  9. Hammer, C. T., and Wills, E. D., 1978, The role of lipid components of the diet in the regulation of the fatty acid composition of the rat liver endoplasmic reticulum and lipid peroxidation, Biochem J., 174:585.Google Scholar
  10. Holman, R. E, 1954, Progress in the chemistry of fats and other lipids, Vol. 2, R. T. Holman, W. O. Lundberg, and T. Malkin, eds., Academic Press, New York.Google Scholar
  11. Kremer, J. M., Bigauoette, J., Michalek, A. V. et al., 1985, Effects of manipulation of dietary fatty acids on clinical manifestations of rheumatoid arthritis, Lancet, i:184.CrossRefGoogle Scholar
  12. Laganiere, S., and Yu, B. P., 1987, Anti-lipoperoxidation action of food restriction, Biochem. Biophys. Res. Comm., 145:1185.CrossRefGoogle Scholar
  13. Lambelet, P., and Loliger, J., 1984, The fate of anti-oxidant radicals during lipid auto-oxidation II. The tocopheroxyl radicals, Chem. Phys. Lipids, 35:184.CrossRefGoogle Scholar
  14. Leslie, C. A., Gonnerman, W. A., Ullman, M. D., Hayes, K. C., Franzblau, C., and Cathcart, E. S., 1985, Dietary fish oil modulates macrophage fatty acids and decreases arthritis susceptibility in mice, J. Exp. Med., 162:1336.CrossRefGoogle Scholar
  15. Liebler, D. C., Kling, D. S., and Reed, D. J., 1986, Antioxidant protection of phospholipid bilayers by α-tocopherol, J. Biol. Chem., 261:12114.Google Scholar
  16. Mouri, K., Ikesu, H., Esaka, T., and Isarashi, O., 1984, The influence of marine oil intake upon levels of lipids, α-tocopherol, and lipid peroxidation in serum and liver of rats, J. Nutr. Sci. Vitaminol., 30:307.CrossRefGoogle Scholar
  17. Ohkawa, H., Ohishi, N., and Yagi, K., 1979, Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction, Anal. Biochem., 95:351.CrossRefGoogle Scholar
  18. Phillipson, B. E., Rothrock, D. W., Connor, W. E., Harris, W. S., and Illingworth, D. R., 1985, Reduction of plasma lipids lipoproteins and apoproteins by dietary fish oils in patients with hypertriglyceridemia, N. Eng. J. Med., 312:1210.CrossRefGoogle Scholar
  19. Prickett, J. D., Robinson, D. R., and Steinberg, A. D., 1981, Dietary enrichment with the polyunsaturated fatty acid eicosapentaenoic acid prevents proteinuria and prolongs survival in NZ1 x NZWF1 mice, J. Clin. Invest., 68:556.CrossRefGoogle Scholar
  20. Siess, W., Scherer, B., Bohlig, B., Roth, P., Kurzmann, I., and Weber, P. C., 1980, Platelet-membrane fatty acids, platelet aggregation, and thromboxane formation during a mackerel diet, Lancet, i:441.CrossRefGoogle Scholar
  21. Stubbs, C. D., and Smith, A. C., 1984, The modification of mammalian membrane polyunsaturated fatty acid composition in relation to membrane fluidity and function, Biochim. Biophys. Acta, 779:89.Google Scholar
  22. Tahin, Q. S., Blum, I., and Carafoli, E., 1981, The fatty acid composition of subcellular membranes of rat liver, heart and brain. Diet-induced modifications, Eur. J. Biochem., 121:5.CrossRefGoogle Scholar
  23. Taylor, S. L., Lambden, M. P., and Tappel, A. L., 1976, Sensitive fluorometric method for tissue tocopherol analysis, Lipids, 11:530.CrossRefGoogle Scholar
  24. Wilson, R. L., 1983, Free radical protection: why vitamin E not Vitamin C, beta-carotene or glutathione?, CIBA Found. Symp., 101:19.Google Scholar
  25. Witting, L. A., and Horwitt, M. K., 1964, Effect of degree of fatty acid unsaturation in tocopherol deficiency-induced creatinuria, J. Nutr., 82:19.Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Serge Laganiere
    • 1
  • Byung P. Yu
    • 1
  • Gabriel Fernandes
    • 1
  1. 1.Department of Medicine and PhysiologyThe University of Texas Health Science Center at San AntonioUSA

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