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Membrane Flow and Endocytosis

  • John Davey

Summary

It is not my intention for this to be an exhaustive review of membrane transport or indeed of endocytosis. These topics have been dealt with extensively elsewhere and the reader is encouraged to browse through them at their leisure (Anderson and Kaplan, 1983; Goldstein et al., 1985; Silverstein et al., 1977; Steinman et al., 1983; Wileman et al., 1985). What I have tried to achieve is a representation of my personal views of membrane transport: highlighting those aspects of transport that are of particular interest to myself. Since my research interests are largely concerned with endocytosis it is from this pathway of membrane transport that I will draw the majority of examples and evidence. I will however include examples from other transport pathways where they are relevant or where they provide insights into transport events that are not observable on the endocytic pathway.

Such a short review cannot of course be exhaustive and I apologise in advance for any ommissions.

Keywords

Cytoplasmic Domain Late Endosome Endocytic Pathway Transport Event Coated Vesicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ahle, S. and Ungewickell, E. (1986) Purification and properties of a new clathrin assembly protein. EMBO J. 5, 3143–3149.PubMedGoogle Scholar
  2. Ahle, S., Mann, A., Eichelsbacher, U. and Ungewickell, E. (1988) Structural relationships between clathrin assembly proteins from the Golgi and the plasma membrane. EMBO J. 7,919–929Google Scholar
  3. Anderson, R.G.W. and Kaplan, J. (1983) Receptor-mediated endocytosis. Modern Cell Biol. 1, 1–52.Google Scholar
  4. Braell, W.A. (1987) Fusion between endocytic vesicles in a cell-free system. Proc. Natl. Acad. Sci. USA 84, 1137–1141.PubMedCrossRefGoogle Scholar
  5. Braell, W.A., Schlossman, D.M., Schmid, S.L. and Rothman, J.E. (1984) Dissociation of clathrin coats coupled to the hydrolysis of ATP:Role of a uncoating ATPase. J. Cell Biol. 99, 734–741.PubMedCrossRefGoogle Scholar
  6. Brown, M.S., Anderson, R.G.W. and Goldstein, J.L. (1983) Recycling receptors:The round-trip itinerary of migrant membrane proteins. Cell 32, 663–667.PubMedCrossRefGoogle Scholar
  7. Davey, J. (1987) A cell-free analysis of the endocytic pathway. Bioscience Reports 7, 299–306.PubMedCrossRefGoogle Scholar
  8. Davey, J., Hurtley, S.M. and Warren, G. (1985) Reconstitution of an endocytic fusion event in a cell-free system. Cell 43, 643–652.PubMedCrossRefGoogle Scholar
  9. Davis, C.G., Lehrman, M.A., Russell, D.W., Anderson, R.G.W., Brown, M.S. and Goldstein, J.L. (1986) The JD mutation in familial hypercholesterolemia:Amino acid substitution in cytoplasmic domain impedes internalization of LDL receptors. Cell 45, 15–24.PubMedCrossRefGoogle Scholar
  10. Davis, C.G., van Driel, I.R., Russell, D.W., Brown, M.S. and Goldstein, J.L. (1987) The low density lipoprotein receptor. Identification of amino acids in cytoplasmic domain required for rapid endocytosis. J. Biol. Chem. 262, 4075–4082.PubMedGoogle Scholar
  11. Diaz, R., Mayorga, L. and Stahl, P. (1987) In vitro fusion of endosomes following receptor-mediated endocytosis. J. Biol. Chem. 263, 6093–6100.Google Scholar
  12. Geuze, H.J., Slot, J.M., Straus, J.A.M., Lodish, H.F. and Schwartz, A.L. (1983) Intracellular site of asialoglycoprotein receptor-ligand uncoupling:Double-label immunoelectron microscopy during receptor-mediated endocytosis. Cell 32, 277–287.PubMedCrossRefGoogle Scholar
  13. Goldstein, J.L., Brown, M.S., Anderson, R.G.W., Russell, D.W. and Schneider, W.J. (1985) Receptor-mediated endocytosis:Concepts emerging from the LDL receptor system. Ann. Rev. Cell Biol. 1, 1–39.PubMedCrossRefGoogle Scholar
  14. Gruenberg, J.E. and Howell, K.E. (1986) Reconstitution of vesicle fusions occurring in endocytosis with a cell-free system. EMBO J. 5, 3091–3101.PubMedGoogle Scholar
  15. Helenius, A., Kartenbeck, J., Simons, K. and Fries, E. (1980) On the entry of Semliki Forest virus into BHK-21 cells. J. Cell Biol. 84, 404–420.PubMedCrossRefGoogle Scholar
  16. Helenius, A., Mellman, I., Wall, D. and Hubbard, A. (1983) Endosomes. Trends Biochem. Sci. 8, 245–250.Google Scholar
  17. Heuser, J. and Evans, L. (1980) Three-dimensional visualisation of coated vesicle formation in fibroblasts. J. Cell Biol. 84, 560–583.PubMedCrossRefGoogle Scholar
  18. Iacopetta, B.J., Rothenberger, S. and Kiihn, L.C. (1988) A role for the cytoplasmic domain in transferrin receptor sorting and coated pit formation during endocytosis. Cell 54, 485–489.PubMedCrossRefGoogle Scholar
  19. Keen, J.H., Willingham, M.C. and Pastan, I.H. (1979) Clathrin coated vesicles:Isolation, dissociation and factor dependent reassociation of clathrin baskets. Cell 16, 303–312.Google Scholar
  20. Lazarovits, J. and Roth, M. (1988) A single amino acid change in the cytoplasmic domain allows the influenza virus hemagglutinin to be endocytosed through coated pits. Cell 53, 743–752.PubMedCrossRefGoogle Scholar
  21. Lehrman, M.A., Goldstein, J.L., Brown, M.S., Russel, D.W. and Schneider, W.J. (1985) Internalization-defective LDL receptors produced by genes with nonsense and frameshift mutations that truncate the cytoplasmic domain. Cell 41, 735–743.PubMedCrossRefGoogle Scholar
  22. Matteoni, R. and Kreis, T.E. (1987) Translocation and clustering of endosomes and lysosomes depends on microtubules. J. Cell Biol. 105, 1253–1265.PubMedCrossRefGoogle Scholar
  23. May, W.S., Sahyoun, N., Jacobs, S., Wolf, M. and Cuatrecasa, P. (1985) Mechanism of phorbol diester induced regulation of surface transferrin receptor involves the action of activated protein kinase C and an intact cytoskeleton. J. Biol. Chem. 260, 9419–9429.PubMedGoogle Scholar
  24. Mostov, K.E., de Bruyn Kops, A. and Deicher, D.L. (1986) Deletion of the cytoplasmic domain of the polymeric immunoglobulin receptor prevents basolateral localization and endocytosis. Cell 47, 359–364.PubMedCrossRefGoogle Scholar
  25. Moya, M., Dautiy-Varsat, A., Goud, B., Louzard, D. and Boquet, P. (1985) Inhibition of coated pit formation in Hep2 cells blocks the cytotoxicity of diptheria toxin but not that of ricin toxin. J. Cell Biol. 101, 548–559.PubMedCrossRefGoogle Scholar
  26. Pearse, B.M.F. (1985) Assembly of the mannose-6-phosphate receptor into reconstituted clathrin coats. EMBO J. 4, 2457–2460.PubMedGoogle Scholar
  27. Pearse, B.M.F. and Robinson, M.S. (1984) Purification and properties of 100kd proteins from coated vesicles and their reconstitution with clathrin. EMBO J. 3, 1951–1957.PubMedGoogle Scholar
  28. Pelham, H.R.B. (1988) Evidence that luminal ER proteins are sorted from secreted proteins in a post-ER compartment. EMBO J. 7, 913–918.PubMedGoogle Scholar
  29. Prywes, R., Livneh, E., Ullrich, A. and Schlessinger, J. (1986) Mutations in the cytoplasmic domain of EGF receptor affect EGF binding and receptor internalization. EMBO J. 5, 2179–2190.PubMedGoogle Scholar
  30. Riezman, H. (1985) Endocytosis in yeast:several of the yeast secretory mutants are defective in endocytosis. Cell 40, 1001–1009.PubMedCrossRefGoogle Scholar
  31. Riezman, H., Chvatchko, Y. and Dulic, V. (1986) Endocytosis in yeast. Trends Biochem. Sci. 11, 325–328.Google Scholar
  32. Robinson, M.S. (1987) 100-kD coated vesicle proteins:Molecular heterogeneity and intracellular distribution studied with monoclonal antibodies. J. Cell Biol. 104, 887–895.Google Scholar
  33. Rothenberger, S., Iacopetta, B.J. and Kühn, L.C. (1987) Endocytosis of the transferrin receptor requires the cytoplasmic domain but not its phosphorylation site. Cell 49, 423–431.PubMedCrossRefGoogle Scholar
  34. Sandvig, K., Olsnes, S., Petersen, O.W. and van Deurs, B. (1987) Acidification of the cytosol inhibits endocytosis from coated pits. J. Cell Biol. 105, 679–689PubMedCrossRefGoogle Scholar
  35. Schlossman, D.M., Schmid, S.L., Braell, W.A. and Rothman, J.E. (1984) An enzyme that removes clathrin coats:Purification of an uncoating ATPase. J. Cell Biol. 99, 723–733.PubMedCrossRefGoogle Scholar
  36. Silverstein, S.C., Steinman, R.M. and Cohn, Z.A. (1977) Endocytosis. Ann. Rev. Biochem. 46, 669–722.CrossRefGoogle Scholar
  37. Steinman, R.M., Mellman, I.S., Muller, W.A. and Cohn, Z.A. (1983) Endocytosis and the recycling of plasma membrane. J. Cell Biol. 96, 1–27.PubMedCrossRefGoogle Scholar
  38. Ungewickell, E. (1983) Biochemical and immunological studies on clathrin light chains and their binding sites on clathrin triskelions. EMBO J. 2, 1401–1408.PubMedGoogle Scholar
  39. Ungewickell, E. and Branton, D. (1981) Assembly units of clathrin coats. Nature 289, 420–422.PubMedCrossRefGoogle Scholar
  40. Wileman, T., Harding, C. and Stahl, P. (1985) Receptor-mediated endocytosis. Biochem. J. 232, 1–14.PubMedGoogle Scholar
  41. Woodman, P.G. and Warren, G. (1988) Fusion between vesicles from the pathway of receptor-mediated endocytosis in a cell-free system. Eur. J. Biochem. 173, 101–108.PubMedCrossRefGoogle Scholar
  42. Woodward, M.P. and Roth, T.F. (1978) Coated vesicles:Characterization, selective dissassociation and reassembly. Proc. Natl. Acad. Sci. USA 75, 4394–4398.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • John Davey
    • 1
  1. 1.Department of BiochemistryUniversity of BirminghamBirminghamUK

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