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Biochemistry of Chemical Carcinogenesis pp 219–226Cite as

Genetic Damage in Peripheral Lymphocytes of Chronic Alcoholics

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Summary

A possible impact of long-term overexposure to ethanol was studied in two groups of chronic alcoholics in a psychiatric hospital. The hypothesis that acetaldehyde may act as a mutagen for somatic cells and induce free radicals in alcoholics tissues was tested. To monitor the amount of injury resulting from alcohol abuse the following markers were used: chromosome aberrations and unscheduled DNA synthesis (UDS) in peripheral lymphocytes and lipid peroxidation (LPO) in plasma and peripheral lymphocytes. The following results were observed: 2.05% aberrant cells (AB.C) in alcoholics vs. 1.29% in the control group; 12 weeks abstinence decreased the frequency of chromosome aberrations from 2.13 X AB.C to 1.52% AB.C. If the patients were supplemented by 1000 mg of ascorbic acid and 400 mg of α-tocopherol per day in the course of 12 weeks, original UDS T/C increased 3.74 to 4.12 (patients without antioxidants = 3.48, controls = 4.72), LPO in plasma decreased from 1.57 to 1.37 nmol MDA/ml (untreated patients = 1.89, controls = 1.12), LPO in lymphocytes decreased from 1.72 to 1.34 nmol/mg protein (untreated patients = 1.96, controls = 1.14).

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Authors and Affiliations

  1. Psychiatric Research Institute, 18103, Prague 8, Czechoslovakia

    R. J. Sram, J. Topinka, B. Binkova, J. Kocisova, V. Kubicek & J. A. Gebhart

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  1. R. J. Sram
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  2. J. Topinka
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  3. B. Binkova
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  4. J. Kocisova
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  5. V. Kubicek
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  6. J. A. Gebhart
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Editor information

Editors and Affiliations

  1. University of York, York, UK

    R. Colin Garner

  2. Research Institute for Tuberculosis and Respiratory Diseases, Prague, Czechoslovakia

    Jan Hradec

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© 1989 Plenum Press, New York

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Sram, R.J., Topinka, J., Binkova, B., Kocisova, J., Kubicek, V., Gebhart, J.A. (1989). Genetic Damage in Peripheral Lymphocytes of Chronic Alcoholics. In: Garner, R.C., Hradec, J. (eds) Biochemistry of Chemical Carcinogenesis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0539-2_25

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  • DOI: https://doi.org/10.1007/978-1-4613-0539-2_25

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-7856-6

  • Online ISBN: 978-1-4613-0539-2

  • eBook Packages: Springer Book Archive

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