Regulation of the Expressed Idiotypic Repertoire in the Normal Immune Response of the Aged

  • Edmond A. Goidl
  • Susan J. Martin McEvoy
  • Francisco A. Bonilla
  • Azad Kaushik
  • Constantin A. Bona
Conference paper
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)


Aging is accompanied by a gradual decline of certain physiological functions. Not the least of these are the effector functions of the immune system. Most aging animals demonstrate a progressive decline in their capacity to mount primary immune responses when compared in magnitude and heterogeneity of antibody affinity with that seen in the immune response of young adult animals.


Aged Mouse Aged Donor Antibody Affinity Primary Immune Response Fetal Liver Cell 
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  1. Geha, R. S., 1982, Presence of auto-anti-idiotypic antibody during the normal human immune response to tetanus toxoid antigen, J. Immunol. 129: 139–144.PubMedGoogle Scholar
  2. Gibbons, J. J., Goidl, E. A., Shepherd, G. M., Thorbecke, G. J., and Siskind, G. W., 1985, Production of auto-anti-idiotypic antibody during the normal immune response. XII. An enzyme-linked immu-nosorbant assay for auto-anti-idiotypic antibody, J. Immunol. Methods 79: 231–237.PubMedCrossRefGoogle Scholar
  3. Goding, J. W., 1976, The chromic chloride method of coupling antigens to erythrocytes: Definition of some important parameters, J. Immunol. Methods 10: 61–66.PubMedCrossRefGoogle Scholar
  4. Goidl, E. A. Innes, J. B., and Weksler, M. E., 1976a, Immunological studies of aging. II. Loss of IgG and high avidity plaque-forming cells and increased suppressor cell activity in aging mice, J. Exp. Med. 144: 1037–1048.Google Scholar
  5. Goidl, E. A., Klass, J., and Siskind, G. W., 1976b, Ontogeny of B-lymphocyte function. II. Ability of endotoxin to increase the heterogeneity of affinity of the immune response of B lymphocytes from fetal mice, J. Exp. Med. 143: 1503–1520.PubMedCrossRefGoogle Scholar
  6. Goidl, E. A., Schrater, A. F., Siskind, G. W., and Thorbecke, G. J., 1979, Production of auto-anti- idiotypic antibody during the normal immune response to TNP-Ficoll. II. Hapten reversible inhibition of anti-TNP plaque-forming cells for auto-anti-idiotypic antibody, J. Exp. Med. 150: 154–165.PubMedCrossRefGoogle Scholar
  7. Goidl, E. A., Thorbecke, G. J., Weksler, M. E., and Siskind, G. W., 1980, Production of auto-anti-idiotypic antibody during the normal immune response: Changes in the auto-anti-idiotypic antibody response and the idiotype repertoire associated with aging, Proc. Natl. Acad. Sci. USA 77: 6788–6792.PubMedCrossRefGoogle Scholar
  8. Gronowicz, E., Coutinho, A., and Moller, G., 1974, Differentiation of B cells: Sequential appearance of responsiveness to polyclonal activators, Scand. J. Immunol. 3: 413–421.PubMedCrossRefGoogle Scholar
  9. Hornbeck, P. V., and Lewis, G. K., 1983, Idiotype connectance in the immune system. I. Expression of a cross reactive idiotype on induced anti-p-azophenyl-arsonate antibodies and on endogenous antibodies not specific for arsonate, J. Exp. Med. 157: 1116–1136.Google Scholar
  10. Jerne, N. K., 1974, Towards a network theory of the immune system, Ann. Immunol. (Paris) 125C: 373–389.Google Scholar
  11. Kaushik, A., Poncet, P., and Bussard, A., 1986, Autoantibodies against bromelainized mouse erythrocyte: Strain distribution of serum idiotype expression and relative peritoneal cell activity, Cell. Immunol. 102: 323–334.PubMedCrossRefGoogle Scholar
  12. Kaushik, A., Lim, A., Poncet, P., Ge, X.-R., and Dighiero, G., 1988, Comparative analysis of natural antibody specificities among hybridomas originating from spleen and peritoneal cavity of adult NZB and BALB/c mice, Scand. J. Immunol. 27: 461–471.PubMedCrossRefGoogle Scholar
  13. Kim, Y. T., Goidl, E. A., Samarut, C., Weksler, M. E., Thorbecke, G. J., and Siskind, G. W., 1985, Bone marrow function. I. Peripheral T cells are responsible for the increased auto-anti-idiotypic response of older mice, J. Exp. Med. 161: 1237–1242.PubMedCrossRefGoogle Scholar
  14. Klinman, N. R., 1981, Antibody-specific immunoregulation and the immunodeficiency of aging, J. Exp. Med. 154: 547–551.PubMedCrossRefGoogle Scholar
  15. Liacopoulos, P., Amstutz, H., and Gille, G., 1971, Early antibody forming cells of double specificity, Immunology 20: 57–66.PubMedGoogle Scholar
  16. Liacopoulos, P., Couder, J., and Bleux, C., 1976, Evidence for multipotentiality of antibody synthesizing cells, Ann. Immunol. (Paris) 127C: 519–530.Google Scholar
  17. McEvoy Martin, S. J., and Goidl, E. A., 1988, Studies on immunological maturation. II. The absence of high-affinity antibody producing cells early in the immune response of the aged is only apparent, Aging Immunol. Infect. Dis. 1: 47–54.Google Scholar
  18. Naor, D., Bonavida, B., Robinson, R. A., Shibata, I. N., Percy, D. E., Chia, D., and Barnett, E. V., 1976, Immune response of New Zealand mice to trinitrophenylated syngeneic mouse red cells, Eur. J. Immunol. 6: 783–789.PubMedCrossRefGoogle Scholar
  19. Reininger, L., Kaushik, A., Izui, S., and Janton, J., 1988, A member of a new VH gene family encodes anti-bromelinized mouse red blood cell autoantibodies, Eur. J. Immunol. 18: 1521–1526.PubMedCrossRefGoogle Scholar
  20. Rittenberg, M. B., and Pratt, K. L., 1969, Anti-trinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen, Proc. Soc. Exp. Biol. Med. 132: 575–581.PubMedGoogle Scholar
  21. Schrater, A. F., Goidl, E. A., Thorbecke, G. J., and Siskind, G. W., 1979, Production of auto-anti-idiotypic antibody during the normal immune response to TNP-Ficoll. I. Occurrence in AKR/J and Balb/c mice of hapten-augmentable anti-TNP plaque-forming cells and their accelerated appearance in recipient of immune spleen cells, J. Exp. Med. 150: 138–153.PubMedCrossRefGoogle Scholar
  22. Sperling, R., Francus, T., and Siskind, G. W., 1983, Degeneracy of antibody specificity, J. Immunol. 131: 882–885.PubMedGoogle Scholar
  23. Szewczuk, M. R., and Campbell, R. J., 1980, Loss of immunocompetence with age may be due to auto- anti-idiotypic antibody, Nature (London) 286: 164–166.CrossRefGoogle Scholar
  24. Talmage, D. W., and Maurer, P. H., 1953, I131-labelled antigen precipitation as a measure of quantity and quality of antibody, J. Infect. Dis. 92: 288–300.PubMedCrossRefGoogle Scholar
  25. Vakil, M., and Kearney, J., 1986, Functional characterization of monoclonal auto-anti-idiotypic antibodies isolated from the early B-cell repertoire of BALB/c mice, Eur. J. Immunol. 16: 1151–1158.PubMedCrossRefGoogle Scholar
  26. Vakil, M., Sauter, H., Paige, C., and Kearney, J., 1986, In vivo suppression of perinatal multispecific B-cells results in a distortion of the adult B-cell repertoire, Eur. J. Immunol. 16: 1159–1165.Google Scholar
  27. Zharhary, D., Segev, Y., and Gershon, H., 1977, The affinity and spectrum of cross-reactivity of antibody production in senescent mice: The IgM response, Mech. Aging. Dev. 6: 385–392.PubMedCrossRefGoogle Scholar
  28. Zharhary, D., Wu, G., and Paige, C., 1988, Utilization of immunoglobulin VH gene families in B cell colonies from aged mice, J. Cell. Biochem. 12B: 102.Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Edmond A. Goidl
    • 1
  • Susan J. Martin McEvoy
    • 1
  • Francisco A. Bonilla
    • 2
  • Azad Kaushik
    • 2
  • Constantin A. Bona
    • 2
  1. 1.Department of Microbiology and Immunology, School of MedicineUniversity of Maryland at BaltimoreBaltimoreUSA
  2. 2.Department of MicrobiologyMount Sinai School of MedicineNew YorkUSA

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