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Simplified Subtraction-Hybridization System for Isolation Of Strain-Specific Rhizobium DNA Probes

  • J. E. Cooper
  • A. J. Bjourson
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 63)

Abstract

Subtraction-hybridization is the name given to procedures for removing nucleic acid sequences from the genome of an organism which are shared with related organisms. Sequences remaining are those which are unique to the organism from which they are derived. Scott et al (1983) used such a technique to isolate particular genes from transformed mouse cells and Welcher et al (1986) extended the concept to bacteria for the isolation of Neisseria gonorrhoeae specific clones. This paper describes the development of subtraction systems for generating Rhizobium DNA sequences capable of discrimination at inter-strain level when used as probes.

Keywords

Neisseria Gonorrhoeae Nucleic Acid Sequence Specific Clone Streptavidin Agarose Step Affinity Chromatography 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Bjourson, A.J. and Cooper, J.E. (1988) Isolation of Rhizobium loti strain-specific DNA sequences by subtraction hybridization. Appl. Environ. Microbiol. 54: 2852–2855.PubMedGoogle Scholar
  2. Scott, M.R.D., Westphal, K-H, and Rigby, P.W.C. (1983) Activation of mouse genes in transformed cells. Cell 34: 557–567.PubMedCrossRefGoogle Scholar
  3. Welcher, A.A., Torres, A.T., and Ward, D.C. (1986) Selective enrichment of specific DNA, cDNA and RNA sequences using biotinylated probes, avidin, and copper-chelate agarose Nucleic Acids Res. 14:10027–10044.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1992

Authors and Affiliations

  • J. E. Cooper
    • 1
    • 2
  • A. J. Bjourson
    • 1
  1. 1.Department of Agriculture for Northern IrelandThe Queen’s University of BelfastBelfastNorthern Ireland
  2. 2.Department of Food and Agricultural MicrobiologyThe Queen’s University of BelfastBelfastNorthern Ireland

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