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Purines and Myocardial Protection pp 481–497Cite as

Ischemic Preconditioning in a Model of Isolated Cardiomyocytes

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Part of the book series: Developments in Cardiovascular Medicine ((DICM,volume 181))

Abstract

An intact heart is an integrated complex of tissues including the cardiomyocyte and interstitial elements (e.g. the extracellular collagen matrix, fibroblasts, mast cells, lymphatics, blood vascular elements and nerves). The complexity of the intact heart often excludes the reliable experimental isolation of single variables which influence cardiomyocyte physiology. The need for a simpler model led to development of methods to isolate cardiomyocytes. Initial attempts produced viable cells from neonatal and fetal hearts, but adult preparations were intolerant of calcium. Powell and Twist (1) first isolated of pure preparations of calcium tolerant adult myocytes. Cardiomyocytes have morphologic, metabolic, electrical and mechanical properties similar to those established for myocytes in intact hearts (2). In addition to the study of normal physiological processes, isolated cardiomyocytes have contributed significantly to understanding of the oxygen paradox, ischemia and reperfusion injury and preconditioning. An understanding of how in vitro cardiomyocyte models contribute to our understanding of cardiac disease might be facilitated by a brief review of these entities.

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Author information

Authors and Affiliations

  1. Department of Veterans Affairs Medical Center and Department of Pathology, East Tennessee State University, James H. Quillen College of Medicine, Box 70568, Johnson City, Tennessee, USA

    Charles E. Ganote & Stephen C. Armstrong

Authors
  1. Charles E. Ganote
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  2. Stephen C. Armstrong
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Editor information

Editors and Affiliations

  1. Cardiothoracic Division, Department of Surgery, Box 890532, MCV Station, Richmond, VA, 23298-0532, USA

    Anwar-Saad A. Abd-Elfattah MS, Ph.D.

  2. Department of Surgery, Medical College of Virginia, Richmond, Virginia, 23298-0532, USA

    Andrew S. Wechsler M.D.

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© 1996 Kluwer Academic Publishers

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Ganote, C.E., Armstrong, S.C. (1996). Ischemic Preconditioning in a Model of Isolated Cardiomyocytes. In: Abd-Elfattah, AS.A., Wechsler, A.S. (eds) Purines and Myocardial Protection. Developments in Cardiovascular Medicine, vol 181. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0455-5_31

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  • DOI: https://doi.org/10.1007/978-1-4613-0455-5_31

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-8056-6

  • Online ISBN: 978-1-4613-0455-5

  • eBook Packages: Springer Book Archive

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