Skip to main content
SpringerLink
Log in

Nonfimbrial Adhesins of Escherichia Coli

  • Chapter
Toward Anti-Adhesion Therapy for Microbial Diseases

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 408))

Abstract

Pathogenic Escherichia coli exhibit a variety of adhesins classified according to their morphology, antigenic structure or receptor specificity.

Morphologically, E. coli adhesins are fimbrial, fibrillar and nonfimbrial. This classification is based on varying appearances in the electron microscope (E.M). Fimbrial adhesins are relatively thick and rigid (5-7nm diameter), while the fibrillar types are much thinner, more flexible and curly (2-3nm diameter). Both can be demonstrated directly by negative staining procedure. A third group of extracellular adhesive structures can be visualized only after stabilization with specific antibodies and then they may possess a capsule-like appearance. (Jann & Hochutzky, 1990, Schmidt, 1994). The latter, generally described as nonfimbrial (NFAs) or A-fimbrial (AFAs), will be described in the following.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Ahrens, R., M. Ott, M. Ritter, H. Hoschutzky et al. Genetic analysis of the gene cluster encoding nonfimbrial adhesin I from Escherichia coli uropathogen. Infect. Immun. 1993, 61: 2505–12.

    PubMed  CAS  Google Scholar 

  • Anstee, D. J. The blood group MNSs active sialoglycoproteins. Semin. Hematol. 1981, 18: 13–27.

    PubMed  CAS  Google Scholar 

  • Aubel, D., A. Darfeuille-Michaud and B. Joly. New adhesive factor (antigen 8786) on a human enteropatho- genic Escherichia coli O117:H4 strain isolated in Africa. Infect. Immun. 1991, 59: 1290–99.

    PubMed  CAS  Google Scholar 

  • Bentz, I. and A. Schmidt. Isolation and serologic characterization of AIDA-I, the adhesin mediating the Diffuse Adherence phenotype of the diarrhea-associated Escherichia coli strain 2787 (O126: H27). Infect. Immun. 1992, 60: 13–18.

    Google Scholar 

  • Donnenberg, M. S. and R.A. Welch. Virulence determinants of uropathogenic Escherichia coli. 1996. In: Urinary Tract Infections. Molecular Pathogenesis and Clinical Management. H. L. T. Mobley and J. W. Warren, eds. ASM Press, Washington. 135–174.

    Google Scholar 

  • Forestier, C., K. W. Welinder, A. Darfeuille-Michaud, and P. Klemm. Afimbrial adhesin from Escherichia coli strain 2230: purification, characterisation and partial covalent structure. FEMS Microbiol. Letters. 1987, 40: 47–50.

    Article  CAS  Google Scholar 

  • Goldhar, J., M. Yavzori, Y. Keisari and I. Ofek. Phagocytosis of Escherichia coli mediated by mannose resistant non_fimbrial hemagglutinin (NFA-1). Microbial Pathogenesis. 1991, 11: 171–78.

    Article  PubMed  CAS  Google Scholar 

  • Goldhar, J., R. Perry, J. R. Golecki, H. Hoschutzky, B. Jann and K. Jann. Nonfimbrial, mannose-rasistant adhesins from uropathogenic Escherichia coli O83:K1:H4 and O14:K?:H11. Infect. Immun. 1987, 55: 1837–42.

    PubMed  CAS  Google Scholar 

  • Grunberg, J., I. Ofek, R. Perry, M. Wiselka, G. Boulnois and J. Goldhar. Blood group NN-dependent phagocytosis mediated by NFA-3 haemagglutinin of Escherichia coli. Immunol. & Infect. Dis. 1994, 4: 28–32.

    CAS  Google Scholar 

  • Grunberg, J., R. Perry, H. Hoschutzky, B. Jann, K. Jann and J. Goldhar. Nonfimbrial blood group N-specific adhesin (NFA-3) from Escherichia coli O20: KX104:H-, causing systemic infection. FEMS Microbiol. Letters. 1988, 56: 241–46.

    Article  Google Scholar 

  • Hales, B. A., H. Beverly-Clarke, N. J. High, K. Jann et al. Molecular cloning and characterization of the genes for a non-fimbrial adhesin from Escherichia coli. Microbial Pathogenesis. 1988, 5: 9–17.

    Article  PubMed  CAS  Google Scholar 

  • Hoschutzky, H., W. Nimmich, F. Lottspeich, and K. Jann. Isolation and characterisation of the non-fimbrial adhesin NFA-4 from uropathogenic Escherichia coli O7: K98: H6. Microbial Pathogenesis. 1989, 6: 351–59.

    Article  PubMed  CAS  Google Scholar 

  • Jann K. and H. Hoschutzky. 1990. Nature and Organization of Adhesins. In: Current Topics in Microbiology and Immunology. Vol. 151. K. Jann and B. Jann, eds. Springer Verlag, Berlin. 55–70.

    Google Scholar 

  • Jann, K. and H. Hoschutzky. 1991. Characterization and surface organization of E. coli adhesins. In: Microbial surface components and toxins in relation to pathogenesis. E.Z. Ron and S. Rotem, eds. Plenum Press, New York. 3–9.

    Google Scholar 

  • Johnson, J. R., K. M. Skubitz, B. J. Nowicki, K.Jacques-Palaz and R. M. Rakita. Nonlethal adherence to human neutrophils mediated by Dr antigen-specific adhesins of Escherichia coli. Infect. Immun. 1995, 63: 309–16.

    PubMed  CAS  Google Scholar 

  • Kahana, H., J. Grunberg, Y. Bartov, R. Perry et al. Binding of Escherichia coli recognising N-blood group antigen to the erythroleukemic cell line K562 expressing glycophorin A. Immunol & Infect. Dis. 1994, 4: 161–165.

    CAS  Google Scholar 

  • Kroncke, K-D., F. Orskov, I. Orskov, B.Jann, K. Jann. Electron microscpe study of coexpression of adhesive protein capsules and polysaccharide capsules in Escherichia coli. Infect. Immun. 1990, 58: 270–275.

    Google Scholar 

  • Labigne-Roussel, A. and S. Falkow. Distribution and degree of heterogeneity of the Afimbrial-adhesin-encoding operon (afa) among uropathogenic Escherichia coli isolates. Infect. Immun. 1988, 56: 640–48.

    PubMed  CAS  Google Scholar 

  • Law, D. Adhesion and its role in the virulence of enteropathogenic Escherichia coli. Clin. Microbiol. Rev. 1994, 7: 152–73.

    PubMed  CAS  Google Scholar 

  • LeBouguenec, C., M. I. Garcia, V. Ouin, J.-M. Dessperrier, P. Gounon, and A. Labigne. Characterization of plasmid-borne afa-3 gene clusters encoding afimbrial adhesins expressed by Escherichia coli strains associated with intestinal or urinary tract infections. Infect. Immun. 1993, 61: 5106–14.

    PubMed  Google Scholar 

  • Maslow, J. N., M. E. Mulligan, K. S. Adams, J. C. Justis and R. D. Arbeit. Bacterial adhesins and host factors: Role in the development and autcome of Escherichia coli bacteremia. Clin. Infect. Dis. 1993, 17: 89–97.

    Article  PubMed  CAS  Google Scholar 

  • Maslow, J. N., T. S. Whittam, C. F. Gliks et al. Clonal relationships among bloodstream isolates of Escherichia coli. Infect. Immun. 1995, 63: 2409–17.

    PubMed  CAS  Google Scholar 

  • Nimmich, W. Adhesins of Escherichia coli. Acta Biotechnol. 1990, 2: 151–61.

    Article  Google Scholar 

  • Nowicki, B., A. Labigne, S. Mosley, R. Hull, S. Hull, and J.Moulds. The Dr hemagglutinin, afimbrial adhesins AFA-I and AFA_III, and Fl845 fimbriae of uropathogenic and diarrhea-associated Escherichia coli belong to a family of hemagglutinins with Dr receptor recognition. Infect. Immun. 1990, 56: 279–81.

    Google Scholar 

  • Nowicki, B., C. Svanborg-Eden, R. Hull, and S. Hull. Molecular analysis and epidemiology of the Dr hemagglutinin of uropathogenic Escherichia coli. Infect. Immun. 1989, 57: 446–451.

    PubMed  CAS  Google Scholar 

  • Ofek, I. and R. Doyle. 1994. Bacterial Adhesion to Cells and Tissues. Chapman and Hall, N.Y. 321–377.

    Google Scholar 

  • Ofek, I., J. Goldhar, Y. Kesari and N. Sharon. Nonopsonic phagocytosis of Microorganisms. Ann. Rev. Microbiol. 1995, 49: 239–76.

    Article  CAS  Google Scholar 

  • Rhen, M., R Klemm and T. Korhonen. Identification of two new hemagglutinins of Escherichia coli N-acetyl-D- Glucosamine specific fimbriae and a blood group M-specific agglutinin, by cloning the corresponding genes in Escherichia coli K-12. J. Bacteriol. 1986, 16: 1234–42.

    Google Scholar 

  • Schmidt, A. M. 1994. Nonfimbrial adhesins of Escherichia coli. In: Fimbriae, Adhesion, Genetics, Biogenesis and Vaccines. R Klemm, ed. C.R.C. Press Inc. 85–96.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Human Microbiology, Tel-Aviv University, Tel-Aviv, Israel

    Janina Goldhar (Sackler Faculty of Medicine)

Authors
  1. Janina Goldhar
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. The Hebrew University — Hadassah Medical School, Jerusalem, Israel

    Itzhak Kahane

  2. Tel Aviv University — Sackler Medical School, Tel Aviv, Israel

    Itzhak Ofek

Rights and permissions

Reprints and permissions

Copyright information

© 1996 Plenum Press, New York

About this chapter

Cite this chapter

Goldhar, J. (1996). Nonfimbrial Adhesins of Escherichia Coli . In: Kahane, I., Ofek, I. (eds) Toward Anti-Adhesion Therapy for Microbial Diseases. Advances in Experimental Medicine and Biology, vol 408. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0415-9_8

Download citation

  • DOI: https://doi.org/10.1007/978-1-4613-0415-9_8

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-8042-9

  • Online ISBN: 978-1-4613-0415-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation