Cell Surface Molecules of Pathogenic and Nonpathogenic Entamoeba Histolytica and their Relation to Virulence

  • David Mirelman
  • Sue Moody-Haupt
  • Steven Becker
  • Yael Nuchamowitz
  • Rivka Bracha
  • Rinat Alon
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 408)

Abstract

In recent years it has become increasingly clear that human infections with Entamoeba histolytica are caused by two types of morphologically indistinguishable trophozoites (McKerrow, 1992; Mirelman, 1992). Symptomatic disease is always associated with the pathogenic type (P), whereas the nonpathogenic (NP) ameba or Entamoeba dispar as many prefer to name it, usually behaves more as a commensal that does not cause symptoms. The correlation, however, between the clinical picture of the patient and the identity of the type of parasite with which he is infected, does not always fit. This complication may be due to the fact that both P and NP types of amebae can sometimes be present in the same stool, as has been recently reported for quite a number of cases (Acuna-Soto, et al. 1993; Walderich et al. 1995).

Keywords

Cholesterol Polysaccharide Trop Amebiasis 

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References

  1. Acuna-Soto, R., Samuelson, J., Girolami, P.D., Zarate, L., Millan-Velasco, F.,Schoolnick,G. and Wirth, D. (1993) Application of the polymerase chain reaction to the epidemiology of pathogenic and nonpathogenic Entamoeba histolytica. Am. J. Trop. Med. Hyg. 48, 58–70.PubMedGoogle Scholar
  2. Bhattacharya, A., Prasad, R. and Sacks, D.L. (1992) Identification and partial characterization of a lipophos-phoglycan from a pathogenic strain of Entamoeba histolytica. Mol Biochem Parasitol 56, 161–168.PubMedCrossRefGoogle Scholar
  3. Bracha, R., Nuchamowitz, Y. and Mirelman, D. (1995) Molecular cloning of a 30-kilodalton lysine-rich surface antigen from a nonpathogenic Entamoeba histolytica strain and its expression in a pathogenic strain. Infect. Immun. 63, 917–925.Google Scholar
  4. Clark, C.G. and Diamond, L.S. (1991) Ribosomal RNA genes of “pathogenic” and “nonpathogenic” Entamoeba histolytica are distinct. Mol. Biochem. Parasitol. 49, 297–302.PubMedCrossRefGoogle Scholar
  5. Garfinkel, L.I., Giladi, M., Huber, M., Gitler, C., Mirelman, D., Revel, M. and Rozenblatt, S. (1989) DNA probes specific for Entamoeba histolytica having pathogenic and non pathogenic zymodemes. Infect. Immun. 57, 926–931.PubMedGoogle Scholar
  6. Grodberg, J., Salazar, N., Oren, R. and Mirelman, D. (1990) Autonomous replication sequences in an extrachromosomal element of a pathogenic Entamoeba histolytica. Nucl. Acids Res. 18, 5515–5519.PubMedCrossRefGoogle Scholar
  7. McKerrow, J.H. (1992) Pathogenesis in amebiasis: Is it genetic or acquired? Infect. Agents and Dis. 1, 11–14.Google Scholar
  8. Mirelman, M. (1992) Pathogenic versus Nonpathogenic Entamoeba histolytica. Infect. Agents and Dis. 1, 15–18.Google Scholar
  9. Moody, S., Becker, S., Nuchamowitz, Y. and Mirelman, D. (1995) Virulent and avirulent Entamoeba histolytica differ in their cell surface phosphorylated glycolipids. The First Meeting of the F.I.S.E.B Eilat, Oct. 1995.Google Scholar
  10. Petri, W.A., Jackson, T.F.H.G., V,G., Kress, K., Saffer, L.D., Snodgrass, T.L., Chapman, M.D., Keren, Z. and Mirelman, D. (1990) Pathogenic and nonpathogenic strains of Entamoeba histolytica can be differentiated by monoclonal antibodies to the galactose-specific adherence lectin. Infect. Immun. 58, 1802–1806.PubMedGoogle Scholar
  11. Ravdin, J.I., Petri, W.A. and Mirelman, D. (1988) Mechanisms of adherence by Entamoeba histolytica. In: Amebiasis: Human Infection by Entamoeba histolytica. Ravdin, J.I. (ed.). John Wiley & Sons, N.Y. (publ), pp. 205–218.Google Scholar
  12. Vines, R.R., Purdy, J.E., Ragland, B.D., Samuelson, J., Mann, B.J. and Petri, W.A. (1995) Stable episomal transfection of Entamoeba histolytica. Mol. Biochem. Parasitol. 71, 265–267.PubMedCrossRefGoogle Scholar
  13. Walderich, B., Muller, Burchard, G.D. and Mirelman, D. (1995) Patients infected with Entamoeba histolytica and Entamoeba dispar as detected by isoenzymes and two different PCR systems. European Conference on Tropical Medicine. Hamburg, Oct. 1995 Abs. LI 19, 150.Google Scholar

Copyright information

© Plenum Press, New York 1996

Authors and Affiliations

  • David Mirelman
    • 1
  • Sue Moody-Haupt
    • 1
  • Steven Becker
    • 1
  • Yael Nuchamowitz
    • 1
  • Rivka Bracha
    • 1
  • Rinat Alon
    • 1
  1. 1.Department of Membrane Research and BiophysicsThe Weizmann Institute of ScienceRehovotIsrael

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