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Effect of Saponins on the Growth and Activity of Rhizosphere Bacteria

  • Robert M. Zablotowicz
  • Robert E. Hoagland
  • Stephen C. Wagner
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 405)

Abstract

Saponins are a group of steroid or triterpenoid glycosides and related chemicals (sapogenins: non-glycosylated) found in roots, shoots, seeds, and flowers of many plant species. Saponins are of agronomic interest because of allelopathic interference with plant growth (Oleszek and Jurzysta, 1987; Waller et al., 1993). Saponins can be released into the soil by secretion from roots and/or leaching from living or decaying plant material (Mishustin and Naumova, 1955; Oleszek and Jurzysta, 1987). Conservation management practices designed to maintain plant residues on the soil surface such as the use of cover crops and reduced tillage are rapidly being adopted by growers; thus the impact of these compounds and other natural products on crop productivity and soil and rhizosphere microbial ecology warrants study.

Keywords

Esterase Activity Extracellular Protein Rhizosphere Bacterium Trichoderma Viride Glycyrrhetic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Banglmm, A.D. and R.W. Home. Action of saponin on biological cell membranes. Nature 196:952 (1962).CrossRefGoogle Scholar
  2. Bradford, M.M. A iapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein—dye binding. AnaL Biochem. 72:248 (1976).PubMedCrossRefGoogle Scholar
  3. Budavari, S. (Ed.) “Escin” #3644, p. 58, in The Merck Index, 11th Edition. Merck and Co., Inc. Rahway, NJ (1989).Google Scholar
  4. Dourmashkin, R.R., R.M. Dougherty, and R J. Harris. Electron microscopic observations on Rous sarcoma virus and cell membranes. Nature 194:1116 (1962).PubMedCrossRefGoogle Scholar
  5. Guilbauk, G.G., and D.N. Kramer. Photometric determination of lipase, acylase, alpha-and gamma—chymotiypsin and inhibitors of these enzymes. AnaL Chem. 36:409 (1964).CrossRefGoogle Scholar
  6. Hoagland, R.E., R.M. Zablotowicz, and M.A. Locke. “Propanil Metabolism by Rhizosphere Microflora”, pp. 160–183 in Bioremediation through Rhizosphere Technology. ACS Symp. Ser. 563, T. A. Anderson and J. R. Coats (Eds.) Am. Chem. Soc., Washington, DC. (1994).CrossRefGoogle Scholar
  7. Hoagland, R.E., R.M. Zablotowicz, and K.N. Reddy. “Studies of the Phytotoxicity of Saponins on Weed and Crop Plants”, this volume (1996).Google Scholar
  8. Levy, M., U. Zehavi, N. Nairn, and I. Polacheck. Isolation, structure determination, synthesis, and antifungal activity of a new alfalfa root saponin. Carbohydr. Res. 193:116 (1989).CrossRefGoogle Scholar
  9. Medzon, E.L., and M.L. Brady. Direct measurement of acetylesterase in living protist cells. J. Bacteriol. 97:402 (1969).PubMedGoogle Scholar
  10. Mishustin, B.N. and A.N. Naumova. Secretion of toxic substances by alfalfa and their effect on cotton and soil microflora. Akad. Nauk USSR Ivestiya, Ser. Biol. 6:3 (Russian) (1955).Google Scholar
  11. Oleszek, W. and M. Jurzysta. The allelopathic potential of alfalfa root medicagenic acid glycosides and their fate in soil environments. Plant Sail 98:67 (1987).CrossRefGoogle Scholar
  12. Polachek, I., U. Zehari, and N. Nairn. Activity of compound G-2 isolated from alfalfa roots against medically important yeasts. Antimicrob. Agents Chemother. 30:290 (1986).Google Scholar
  13. Pollock, C.R. “Exoenzymes”, in The Bacteria, Vol. 1. R. Y. Stainer and I. C. Gunsalus (Eds.) Academic Press, New York (1964).Google Scholar
  14. Rovira, A.D. and C.B. Davey. “Biology of the Rhizosphere”, pp. 153–204 in The Plant Root. Environment. E.W. Carlson, (Ed.) University Press, Charlottesville, Va. (1974).Google Scholar
  15. Schnurer, J, and T. Rosswall. Fluorescein diacetale hydrolysis as a measure of total microbial activity in soil and litter. Appl Environ, Microbiol. 43:1256 (1982).Google Scholar
  16. Timbekova, A.E., M.I. Isaev, and N.K. Abubakirov. “Chemistry and Biological Activity of Triterpenoid Glycosides from Medicago sativa”. this volume (1996).Google Scholar
  17. Waller, G.R., M. Jurzysta, and R.L. Z. Thorne. Root saponins from alfalfa (Medicago sativa L.) and their allelopathic activity on weeds and wheat. Bot. Bull Acad. Sin. 34:1 (1993).Google Scholar
  18. Wagner, S.C., R.M. Zablotowicz, M.A. Locke, R.J. Smeda, and C.T. Bryson. Influence of herbicide—desiccated cover crops on biological soil quality in the Mississippi Delta. Proc. Conservation Tillage Conference for Sustainable Agriculture, MAFES Special Bulletin 88:86 (1995).Google Scholar
  19. Zablotowicz, R.M., R.E. Hoagland, M.A. Locke, and W.J. Hickey. Glutathione-S-transferase activity and metabolism of glutathione conjugates by rhizosphere bacteria. AppL Environ. Microbiol. 61:1054 (1995).PubMedGoogle Scholar
  20. Zentmyer, G.A. Biological control of Phytophthora root rot of avocado with alfalfa meal. Phytopathology 53:1383 (1963).Google Scholar
  21. Zentmyer, G. A. and G.R. Thompson. The effect of saponins from alfalfa on Phytophthora cinnamomi in relation to control of root rot of avocado. Phytopathology 57:1278 (1967).Google Scholar
  22. Zimmer, D.E., M.W. Pederson, and C.F. McGuire. A bioassay for alfalfa saponins using the fungus Trichoderma viride pers. ex. Fr. Crop Sci. 7:223 (1967).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1996

Authors and Affiliations

  • Robert M. Zablotowicz
    • 1
  • Robert E. Hoagland
    • 1
  • Stephen C. Wagner
    • 1
  1. 1.Southern Weed Science LaboratoryUSDA-ARSStonevilleUSA

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