Saponins from Alfalfa, Clover, and Mungbeans Analyzed by Electrospray Ionization-Mass Spectrometry as Compared with Positive and Negative Fab-Mass Spectrometry
Saponins are mixtures of several triterpenoid glycosides and can serve as allelochemicals. The variety of biological properties inherent in saponins has attracted interest in the development of new analytical methodologies. Mass spectrometry has been used for determination and structural characterization of saponins. They were ionized by ionization modes such as electron impact (EI)1, chemical ionization (CI)2, field desorption (FD)3, Californium plasma desorption (252Cf-PDMS)4, fast atom bombardment (FAB)5,6, and energetic ions (LSIMS)7 coupled with liquid chromatography using thermospray (TSP) ionization8 and reported in this book by ESI9,10. In this paper, we reported using electrospray ionization (ESI) to obtain structural information on saponins. Samples of soyasaponins from mungbeans, Vigna radiata L. (I and II), root saponins from Medicago sativa L. Cimarron cultivar grown in Oklahoma (III), and seed saponins from Trifolium incarnatum L. (IV-VII) were analyzed by ESI/MS and FAB/MS. The results were compared with the positive and negative FAB to illustrastrate the potentials of using ESI/MS to characterize saponins.
KeywordsFast Atom Bombardment Medicago Sativa Vigna Radiata Fast Atom Bombardment Mass Spectrometry Field Desorption
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