Liquid Secondary Ion Mass Spectrometry and Linked Scanning at Constant B/E LSIMS/MS for Structure Confirmation of Saponins in Medicago Sativa (ALFALFA)

  • P. R. West
  • G. R. Waller
  • P. W. Geno
  • W. Oleszek
  • M. Jurzysta
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 405)


Mass spectrometry has been used extensively for saponin determination and structural confirmation. Sapogenins (saponin aglycones) were first studied by Budzikiewicz and coworkers using electron impact (EI) ionization.1 Hostettmann utilized desorption/chemical ionization mass spectrometry for the structure determination of triterpenoid and spirostanol saponins.2 Schulten and Soldati applied field desorption mass spectrometry for the confirmation structure of saponins from Gleditsia japonica.3 Mostad and Doehl used liquid chromatography mass spectrometry (LC-MS) with chemical ionization (CI) for the characterization of sapogenins from Gypsophila arrostii. 4 Massiot et al. used californium plasma desorption mass spectrometry (252Cf-PDMS) for structure elucidation of alfalfa root saponins.5 Numerous investigators have used fast atom bombardment (FAB) or LSIMS for saponin determination.6,7


Fast Atom Bombardment Collisionally Activate Dissociation Fast Atom Bombardment Mass Spectrometry Charge Retention Collisionally Activate Dissociation Spectrum 
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Copyright information

© Plenum Press, New York 1996

Authors and Affiliations

  • P. R. West
    • 1
  • G. R. Waller
    • 2
  • P. W. Geno
    • 3
  • W. Oleszek
    • 4
  • M. Jurzysta
    • 4
  1. 1.Structural Chemistry, Department 418, AP31Abbott LaboratoriesAbbott ParkUSA
  2. 2.Department of Biochemistry and Molecular BiologyOklahoma State UniversityStillwaterUSA
  3. 3.Department of Environmental ChemistrySouthwest Research InstituteSan AntonioUSA
  4. 4.Department of BiochemistryInstitute of Soil Science and Plant CultivationPulawyPoland

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