Abstract
Opiate alkaloid-selective receptors, insensitive to opioid peptides and differing from classical opioid receptors in other respects, mediate inhibition by morphine of activation of human peripheral blood monocytes and are present on other macrophage cell types as well, including microglia. This receptor has been tentatively designated as a µ3 subtype. The present studies provide evidence for the presence of this receptor in cultures of astrocytes derived from human fetal brain, in cultures of feline astrocytes and in an immortalized feline astrocyte cell line. Also, neonatal and adult rat striatum and other brain regions, assayed in the presence of agents that blocked classical µ, δ and κ binding sites, were found to contain additional saturable 3H-morphine binding sites that exhibited the pharmacology of µ3 receptors. Additionally, µ3 receptor sites were present in rat retina and in the avascular guinea pig retina, retinas that lack endogenous opioid peptides and that do not contain classical opioid peptide-sensitive µ, δ or κ binding sites. For each of these preparations, with 3H-morphine as ligand, binding sites were saturable and displaced by morphine, etorphine, naloxone, diprenorphine and morphine 6-glucuronide, but not by morphine 3-glucuronide, fentanyl, benzomorphans, or by enkephalins, dynorphin, β-endorphin or other opioid peptides. These sites also could be distinguished from other (µ, δ and κ) sites on the basis of sensitivity to sulfhydryl compounds. It is proposed that.µ3 receptors occur on astrocytes as well as on microglia in brain and retina, where they function to modulate responses to injury and infection, as has been proposed previously for these receptors in the immune system.
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References
Stefano, G. B., Digenis, A., Spector, S., Leung, M. K., Bilfinger, T., Makman, M. H., Scharrer, B. and Abumrad, N. N. (1993). Opiate-like substances in an invertebrate, an opiate receptor on invertebrate and human immunocytes, and a role in immunosuppression. Proc. Natl. Acad. Sc.i U.S.A. 90: 11099–11103.
Makman, M. H., Bilfinger, T. V. and Stefano, G. B. (1995) Human granulocytes contain an opiate alkaloid-selective receptor mediating inhibition of cytokine-induced activation and chemotaxis. J. Immunol. In Press 154: 1323–1330.
Makman, M. H., Dvorkin, B. and Stefano, G. B. (1995) Murine macrophage cell lines contain p.3 opiate receptors. Eur. J. Pharmacol. 273: R5–R6.
Makman, M. H. (1994) Morphine receptors in immunocytes and neurons. Adv. Neuroimmunol. 4: 69–82.
Dobrenis, K., Makman, M H and Stefano, G. B. (1995) Occurrence of the opiate alkaloid-selective 1.13 receptor in mammalian microglia and astrocytes in culture and in Kupffer cells. Brain Res. 686: 239–248.
Cruciani, R. A., Dvorkin, B., Klinger, H. P. and Makman, M. H. (1994) Presence in neuroblastoma cells of a µ3 receptor with selectivity for opiate alkaloids but without affinity for opioid peptides. Brain Res. 667: 229–237.
McCarthy, K. D. and deVellis, J. (1980) Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. J. Cell Biol. 85: 890–902.
Small, R. E., Watkins, B. A., Munro, P.M. and Liu, D. (1993) Functional properties of retinal Müller cells following transplantation to the anterior eye chamber. Glia 7: 158–169.
Benveniste, E.N. (1993) Astrocyte-microglia interactions. Chapter in S. Murphy, ed.:Astrocytes-Pharmacology and Function, Academic Press, NY, pp. 355–382.
Eddleston, M. And Mucke, L. (1993) Molecular profile of reactive astrocytes-implications for their role in neurologic disease. Neuroscience 54: 15–36.
Landis, D. M. D. (1994) The early reactions of non-neuronal cells to brain injury. Annu. Rev. Neurosci. 17: 133–151.
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© 1996 Plenum Press, New York
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Makman, M.H., Dobrenis, K., Downie, S., Lyman, W.D., Dvorkin, B. (1996). Presence of Opiate Alkaloid-Selective μ3 Receptors in Cultured Astrocytes and in Brain and Retina. In: Friedman, H., Eisenstein, T.K., Madden, J., Sharp, B.M. (eds) AIDS, Drugs of Abuse, and the Neuroimmune Axis. Advances in Experimental Medicine and Biology, vol 402. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0407-4_4
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DOI: https://doi.org/10.1007/978-1-4613-0407-4_4
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