Skip to main content
SpringerLink
Log in

Humoral Immune Response to Human Cytomegalovirus

Diagnostic and Clinical Implications

  • Chapter
Molecular Biology of Hematopoiesis 5

  • 99 Accesses

Abstract

Human Cytomegalovirus (CMV) is associated with several diseases in immunocompromised individuals. Diagnosis of CMV infection can be obtained by direct demonstration of the virus or virus components in pathological materials or indirectly through serology. Serological diagnosis gives only indirect evidence of the presence of the virus, and is problematic because of the immunological disorders occurring in most patients at risk of developing a CMV infection. Furthermore, antigenic reagents used in commercially available kits are not standardized and discordant results are often obtained. However serology is cheaper than the other diagnostic tests, requires a short execution time, is safe and can be completely automized.

Furthermore the rapid evolution triggered by the detailed study of the viral genome and its antigenic gene products has allowed a rapid progress in CMV serology. Therefore, it is worthwhile exploring the possible application fields in which the use of serology is justified now adays. This is what this review will attempt to do in general terms and in bone marrow transplant recipients in particular.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. . Ho M Cytomegalovirus. Biology and Infection. II Edition. Plenum Medical Press, New York and London, 1991.

    Google Scholar 

  2. . Britt, W.J. Infections associated with human cytomegalovirus. Chapter 2 of “Herpesvirus Infections” R. Glaser and J.F. Jones Editors, Marcel Dekker, Inc, New York. 1994.

    Google Scholar 

  3. . Landini M P. New approaches and perspectives in cytomegalovirus diagnosis. Prog Med Virol 1993; 4, 157 – 177.

    Google Scholar 

  4. . Epstein JB, Phillips K, Sherlock CH Viral serology after bone marrow transplantation. Viral immunol 1991; 4: 133 – 137.

    Article  PubMed  CAS  Google Scholar 

  5. . The TH, van der Berg AP, van Son WJ, et al. Recent advances in the early and reliable immunodiagnosis of cytomegalovirus infection in immunocompromised hosts, in Landini MP, ed: Progress in Cytomegalovirus Research, Elsevier, Amsterdam, 1991: 209.

    Google Scholar 

  6. . Alford, CA, Hayes K, Britt W. Primary cytomegalovirus infection in pregnancy: Comparison of antibody responses to virus-encoded proteins between women with and without intrauterine infection. J Inf Dis 158: 917 – 924, 1988.

    Article  CAS  Google Scholar 

  7. . Boppana, SB, Pass R, Britt W. Virus-specific antibody responses in mothers and their newborn infants with asymptomatic congenital infections. J Inf Dis 167: 72 – 77, 1993.

    Article  CAS  Google Scholar 

  8. . Blackburn NK, Besselaar TG, Schoub BD, O’Connell KF. Differentiation of primary cytomegalovirus infection from reactivation using the urea denaturation test for measuring antibody avidity. J Med Virol 33: 6 – 9, 1991.

    Article  PubMed  CAS  Google Scholar 

  9. . Gleaves CA, Wendt SF, Dobbs DR, Meyers JD. Evaluation of the CMV-CUBE assay for detection of Cytomegalovirus serological status in marrow transplant patients and marrow donors. J Clin Microbiol 28: 841 – 842, 1990.

    PubMed  CAS  Google Scholar 

  10. . Koerner K, Kilian D, Zimmermann B, Nebel Schickel H, Horn J. Comparison of four different ELISAs and indirect immunofluorescence for screening of blood donors for antibodies to Cytomegalovirus. Biotest Bull 4: 119 – 123, 1990.

    Google Scholar 

  11. . Kraat YJ, Hendrix RMG, Landini MP, Bruggeman CA. Comparison of four techniques for detection of antibodies to Cytomegalovirus. J Clin Microbiol 30: 522 – 524, 1992.

    PubMed  CAS  Google Scholar 

  12. . Gutierrez J, del Carmen Maroto C, Piedrola G. Evaluation of a new reagent for anti-cytomegalovirus and anti-Epstein barr Virus immunoglobulin G. J Clin Microbiol 32: 2603 – 2605, 1994.

    PubMed  CAS  Google Scholar 

  13. . Doerr HW and Albert S. New developments in CMV antibody screening. Biotest Bull 4: 125 – 130, 1990.

    Google Scholar 

  14. . Landini, M.P. Antibody response to human cytomegalovirus proteins. Rev Med Virol 2: 63 – 72, 1992

    Article  CAS  Google Scholar 

  15. . Landini MP, Rossier E, Schmitz H. Antibodies to human cytomegalvirus structural polypeptides during primary infection. J Virol Meth 22: 309–317, 1988,

    Article  CAS  Google Scholar 

  16. . Vornhagen R, Plachter B, Hinderer W, The TH, Van Zanten J, Matter L, Schmidt CA, Sonneborn HH, Jahn G. Early serodiagnosis of acute cytomegalovirus infection by Enzyme-linked immunosorbent assay using recombinant antigens. J Clin Microbiol 32: 981 – 986, 1994.

    PubMed  CAS  Google Scholar 

  17. . Landini MP, Guan MX, Jahn G, Lindenmeier W, Mach M, Ripalti A, Necker A, Lazzarotto T, Plachter B. Large scale screening of human sera with Cytomegalovirus recombinant antigens. J Clin Microbiol 28: 1375 – 1379, 1990

    PubMed  CAS  Google Scholar 

  18. . Scholl BC, Von Hintzestein B, Borisch B, Traupe B, Broker M, Jahn G. Procaryotic expression of immunogenic polypeptides of the large phosphoprotein (ppl50) of human cytomegalovirus. J Gen Virol 69: 1195 – 1204, 1988

    Article  PubMed  CAS  Google Scholar 

  19. . Novak J, Sova P, Krchnak V, Hamsikova E, Zavadova H E Mapping of serologically relevant regions of human cytomegalovirus phosphoprotein pp150 using synthetic peptides. J Gen Virol 72: 1409 – 1413, 1991

    Article  PubMed  CAS  Google Scholar 

  20. . Plachter B, Wieczorek L, Scholl B-C, Ziegelmaier R and Jahn G. Detection of Cytomegalovirus antibodies by an enzyme linked immunosorbent assay using recombinant polypeptides of the large phosphorylated tegument protein pp150. J Clin Microbiol 30: 201 – 206, 1992

    PubMed  CAS  Google Scholar 

  21. . Meyer H, Bankier AT, Landini MP, Brown CM, Barrell BG, Ruger B, Mach M. Identification and procaryotic expression of the gene coding for the highly immunogenic 28Kilodalton st5ructural phosphoprotein (pp28) of human cytomegalovirus. J Virol 62: 2243 – 2250, 1988.

    PubMed  CAS  Google Scholar 

  22. . Vornhagen R, Plachter B, Hinderer W, Bein G, The TH, Matter L, Sonneborn HH, Jahn G. Serodiagnosis of acute and past HCMV-infection using recombinant autologous fusion proteins. Cytomegalovirus workshop, Stockholm 1995

    Google Scholar 

  23. . Snydman DR. Cytomegalovirus Immunoglobulins in the prevention and treatment of Cytomegalovirus disease. Rev Inf Dis 12 (sup7): 839–848.

    Google Scholar 

  24. . Mach M. and Britt W Immunoprophylaxix of human cytomegalovirus infections in Progress in Progress in Cytomegalovirus Research, MP Landini Ed, 1991, Elsevier, Amsterdam.

    Google Scholar 

  25. . Utz U, Britt WJ, Vugler L, Mach M. Identification of a neutralizing epitope on glycoprotein gp58 of human cytomegalovirus. J Virol 63: 1995 – 2001, (1989).

    PubMed  CAS  Google Scholar 

  26. . Rasmussen L, Matkin C, Spaete R, Pachi C, merigan TC. Antibody response to human cytomegalovirus glycoprotein B gB and gH after natural infection in humans. J Inf Dis 164: 835 – 842, 1991.

    Article  CAS  Google Scholar 

  27. . Marshall GS, Rabalais GP, Stout GG, Waldeyer SL. (1992) Antibodies to recombinant-derived glycoprotein B after natural cytomegalovirus infection correlate with neutralizing activity. J Inf Dis 165: 381–384, 1992.

    Article  CAS  Google Scholar 

  28. . Kniess N, Mach M, Fay J Britt WJ. Distribution of linear antigenic sites on glycoprotein gp55 of human cytomegalovirus. J Virol 65: 138 – 146, 1991.

    PubMed  CAS  Google Scholar 

  29. . Meyer H, Sundqvist VA, Pereira L, Mach M. Glycoprotein gp116 of human cytomegalovirus contains epitopes strain common and strain-specific antibodies. J Gen Virol 73: 2375 – 2383, 1992.

    Article  PubMed  CAS  Google Scholar 

  30. . Silvestri M, Sundqvist VA, Ruden U, Wharen B. Characterization of a major antigenic region on gp55 of human cytomegalovirus. J Gen Virol 72: 3017 – 3023, 1991.

    Article  PubMed  CAS  Google Scholar 

  31. . Wagner B, Kropff B, Kalbacher H, Britt WJ, Sundqvist VA, Ostberg L, Mach M. A continuous sequence of more than 70 aminoacids is essential for antibody binding to the dominant antigenic site of glycoprotein gp58 of human cytomegalovirus. J Virol 66: 5290 – 5297, 1992a.

    CAS  Google Scholar 

  32. . Ohlin M, Sundqvist, VA, Gilljam G, Ruden U, Gombert FO, Wharen B, Borrebaeck CA. Characterization of human monoclonal antibodies directed against the pp65 matrix antigen of human cytomegalovirus. Clin Expt Immunol 84: 508 – 514, 1991.

    CAS  Google Scholar 

  33. . Kropff B, Landini MP, Mach M. An Elisa using recombinant proteins for the detection of neutralizing antibodies against human cytomegalovirus. J Med Virol 39: 187 – 195, 1993.

    Article  PubMed  CAS  Google Scholar 

  34. . Urban M, Britt WJ, Mach M. The dominant linear neutralizing antibody-binding site of glycoprotein gp86 of human cytomegalovirus is strain-specific. J Virol 66: 1303 – 1311, 1992.

    PubMed  CAS  Google Scholar 

  35. . Simpson JA, Chow JC, Baker J, Avdalovic N, Yuan S, Au D, Co MS, Vasquez M, Britt WJ, Coelingh KL. Neutralizing monoclonal antibodies that distinguish three antigenic sites on human glycoprotein H have distintict binding sites. J Virol 67: 489 – 496, 1993.

    PubMed  CAS  Google Scholar 

  36. . Lazzarotto T, Dalla Casa B, Campisi B, Landini MP. Enzyme-linked immunoadsorbent assay for the detection of cytomegalovirus-IgM: Comparison between eight commercial kits, immunofluorescence and immunoblotting. J Clin Lab Anal 1992; 6: 216 – 218.

    Article  PubMed  CAS  Google Scholar 

  37. . Landini MP, Ripalti A, Sra K, Pouletty P. Human cytomegalovirus structural proteins: immune raction against pp150 synthetic peptides. J Clin Microbiol 1991; 29: 1868 – 1872.

    PubMed  CAS  Google Scholar 

  38. . Ripalti A, Ruan Q, Boccuni MC, Campanini F, Bergamini G and Landini MP Construction of a polyepitope fusion antigens of human cytomegalovirus ppUL32: reactivity with human antibodies J Clin Microbiol 1994; 32: 358 – 363.

    CAS  Google Scholar 

  39. . Ripalti A, Boccuni MC, Campanini F, Bergamini G, Lazzarotto T, Battista MC, Dalla Casa B, Landini MP. Construction of a polyepitope fusion antigen of human cytomegalovirus ppUL32 and detection of specific antibodies by ELISA. Microbiologica 18: 1 – 12, 1994

    Google Scholar 

  40. . Ripalti A, Dal Monte P, Boccuni, MC, Campanini, F, Lazzarotto T, Campisi B, Ruan Q, Landini MP. Prokaryotic expression of a large fragment of the most antigenic cytomegalovirus DNA-binding protein (ppUL44) and its reactivity with human antibodies. J Virol Methods 1994; 46: 39 – 50.

    Article  PubMed  CAS  Google Scholar 

  41. . Lindenmeier W, Necker A, Krause S, Bonewald R, Collins J. Cloning and characterization of major antigenic determinants of human cytomegalovirus AD169 seen by the human immune system. Arch Virol 1990; 113: 1 – 16.

    Article  Google Scholar 

  42. . Vornhagen, R, Hinderer, W, Sonneborg, HH, Bein, G, Matter, L, The, TH, Jahn, G, Plachter, B. The DNA-binding protein pUL57 of Human Cytomegalovirus is a major terget antigen for the immunoglobulin M antibody response during acute infection. J Clin Microbiol 33: 1927 – 1930, 1995.

    PubMed  CAS  Google Scholar 

  43. . Maine, GT, Lazzarotto, T, Chovan, LE, Flanders, R, and Landini, MP. The DNA-binding protein pUL57 of human Cytomegalovirus: Comparison of specific Immunoglobulin M (IgM) reactivity with IgM reactivity to other major terget antigens. Clin Diag Lab Immunol 3: 358 – 360, 1996.

    CAS  Google Scholar 

  44. . Landini MP, Lazzarotto T, Maine GT, Ripalti A, Flanders R. recombinant mono and poly antigens to detect Cytomegalovirus-specific IgM in human sera by enzyme immunoassay. J Clin Microbiol 1995; submitted.

    Google Scholar 

  45. . Weber W, Nestler U, Ernst W, Rabenau H, Braner J, Birkenbach A, Scheuermann EH, Schoeppe W, Doerr HW. Low correlation of human cytomegalovirus DNA amplification byn polymerase chain reaction with cytomegalovirus disease in organ transplant recipients. J Med Virol. 1994; 43: 187 – 193.

    Article  PubMed  CAS  Google Scholar 

  46. . Ghisetti V, Barbui A, Lazzarotto T, Donegani E, Ripalti A, Dal Monte P Bobbio, di Summa M, Marchiaro G, Landini MP. Comparison between virology and serology for the follow upof cytomegalovirus infection in heart transplant recipients. Transplantation, submitted.

    Google Scholar 

  47. . Landini MP, Lazzarotto T, Ripalti A, Guan MX, La Placa M. Antibody response to recombinant Lambda gt11 fusion proteins in Cytomegalovirus infection. J Clin Microbiol 1989; 27: 2324 – 2327.

    PubMed  CAS  Google Scholar 

  48. . Basson J, Tardy JC, Aymard M. Characterization of immune complexes containing Cytomegalovirus-specific IgM antibodies following a kidney graft. J Med Virol 1991; 33: 205 – 210.

    Article  PubMed  CAS  Google Scholar 

  49. . Strand OA, Hoddevik GM The diagnostic significance of specific serum IgA detection in Cytomegalovirus infection. Archives of Virology 1984; 82: 173 – 180.

    Article  PubMed  CAS  Google Scholar 

  50. . Morris GE, Coleman RM, Best JM, Benetato BB, Nahmias AJ Persistence of serum IgA antibodies to herpes simplex, Varicella Zoster, Cytomegalovirus and Rubella virus detected by enzyme-linked immunosorbent assays. Journal of Medical Virology 1985; 16: 343 – 349.

    Article  PubMed  CAS  Google Scholar 

  51. . Vargas, MA, Bertrand, F, Mulongo, N, Squifflet, JP, Lamy, ME. Specific IgE detected by ELISA and immunoblott after human cytomegalovirus infection in renal transplant recipients. Clin Diag Virol 6: 1 – 9, 1996.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Section of Microbiology Department of Clinical and Experimental Medicine, University of Bologna, Italy

    Maria Paola Landini MD, Tiziana Lazzarotto & Paola Dal Monte

  2. Institute of Microbiology, University of Bologna, St. Orsola General Hospital, Via Massarenti n.9, 40138, Bologna, Italy

    Maria Paola Landini MD

Authors
  1. Maria Paola Landini MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tiziana Lazzarotto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Paola Dal Monte
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. The Rockefeller University, New York, New York, USA

    Nader G. Abraham

  2. University of Tokyo, Tokyo, Japan

    Shigetaka Asano

  3. Universität-Gesamthoschschule Essen, Essen, Germany

    Günther Brittinger

  4. Istituto Cantonale di Patologia, Locarno, Switzerland

    Georges J. M. Maestroni

  5. Western Pennsylvania Cancer Institute, Pittsburgh, Pennsylvania, USA

    Richard K. Shadduck

Rights and permissions

Reprints and permissions

Copyright information

© 1996 Plenum Press, New York

About this chapter

Cite this chapter

Landini, M.P., Lazzarotto, T., Monte, P.D. (1996). Humoral Immune Response to Human Cytomegalovirus. In: Abraham, N.G., Asano, S., Brittinger, G., Maestroni, G.J.M., Shadduck, R.K. (eds) Molecular Biology of Hematopoiesis 5. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0391-6_38

Download citation

  • DOI: https://doi.org/10.1007/978-1-4613-0391-6_38

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-8031-3

  • Online ISBN: 978-1-4613-0391-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation