Abstract
A Hut-78 celi clone (F12) harboring a non-producer human immunodeficiency virus (HIV-1) variant shows complete resistance to HIV-1 or HIV-2 superinfection. The F12-HIV provirus produces an altered HIV-1 protein pattern and cannot generate even immature viral particles. We demonstrated that HeLa CD4+ cells transfected with the F12-HIV genome resist HIV superinfection through a CD4-independent mechanism. As F12-HIV appears to be a useful system to induce anti-HIV intracellular immunization, we constructed various retroviral vectors containing the F12-HIV genome, modified by elimination of the F12 3’ LTR and part of its nef gene, inserted “antisense” with respect to the Moloney murine leukemia virus 5’ LTR. Here we show that recombinant retroviral particles carrying the N2/F12-HIV nef-(as) construct can stably transduce both CEMss human cells and primary human peripheral blood lymphocytes, inducing the expression of the F12-HIV genome. These results could open the way to an anti-AIDS gene therapy strategy based on F12-HIV-induced intracellular immunization.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
. Federico M, Titti F, Buttò S, Orecchia A, Carlini F, Taddeo B, Macchi B, Maggiano N, Verani P, Rossi GB: Biologic and molecular characterization of producer and non producer clones from HUT-78 cells infected with a patient HIV isolate. AIDS Res Hum Retrov 5: 385 – 396, 1989.
. Federico M, Nappi F, Bona R, D’Aloja P, Verani P, Rossi GB: Full expression of transfected non-producer interfering HIV-1 proviral DNA abrogates susceptibility of human HeLa CD4+ cells to HIV. Virology 206: 76 – 84, 1995.
. Carlini F, Federico M, Equestre M, Ricci S, Ratti G, Zibai Q, Verani P, Rossi GB: Sequence analysis of an HIV-1 proviral DNA from a non producer chronically infected HUT-78 cellular clone. J. Viral Diseases 1: 40 – 55, 1992.
. Rossi GB, Verani P, Macchi B, Federico M, Orecchia A, Nicoletti L, Buttò S, Lazzarin A, Mariani G, Ippolito G, Manzari V: Recovery of HIV-related retroviruses from Italian patients with AIDS or AIDS-related complex and from asymptomatic at-risk individuals. Ann NY Acad Sci USA 511: 390 – 400, 1987.
. Wigier M, Sweet R, Sim GK, Wold B, Pellicer A, Lacy E, Maniatis T, Silverstein S, Awel R: Transformation of mammalian cells with genes from procaryotes and eucaryotes. Cell 16: 758 – 777, 1979.
. Federico M, Taddeo B, Carlini F, Nappi F, Verani P, Rossi GB: A recombinant retrovirus carrying a non-producer human immunodeficiency virus (HIV) type 1 variant induces resistance to superinfecting HIV. J Gen Virol 74: 2099 – 2110, 1993.
. Lu Y, Touzjian N, Stensel M, Dorfman T, Sodroski JG, Haseltine WA: Identification of cis-acting repressive sequences within the negative regulatory element of human immunodeficiency virus type 1. J Virol 64: 5226 – 5229, 1990.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Plenum Press, New York
About this chapter
Cite this chapter
Federico, M. et al. (1996). Anti-Hiv Viral Interference Induced by Retroviral Vectors Expressing a Non-Producer Hiv-1 Variant. In: Abraham, N.G., Asano, S., Brittinger, G., Maestroni, G.J.M., Shadduck, R.K. (eds) Molecular Biology of Hematopoiesis 5. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0391-6_35
Download citation
DOI: https://doi.org/10.1007/978-1-4613-0391-6_35
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-8031-3
Online ISBN: 978-1-4613-0391-6
eBook Packages: Springer Book Archive