Abstract
Pretransplant screening of potential recipient patient sera to determine the level of allosensitization to class I HLA antigens has traditionally been done as an aid in the management of transplant waiting lists. A soluble HLA (sHLA) ELISA was developed as an alternative to lymphocytotoxicity for anti-HLA antibody screening. This ELISA was used for the detection of anti-HLA class I IgG, IgGl, IgG2, IgG3, IgG4, and IgM antibodies and used to analyze the correlation of pretransplant allosensitization and post transplant rejection episodes in renal allograft recipients. ELISA plates were coated with 46 different sHLA preparations representing 40 different HLA class I antigens. After incubation with a serum specimen, bound antibodies were detected with a peroxidase conjugated antibody. Serum specimens from 158 microlymphocytotoxicity positive patients were analyzed. Eighty-four patients had experienced one or more rejection episodes within twelve months post trans-plantation. One hundred two patients were tested positive by ELISA (total IgG %PRA > 10%). A strong correlation between first year rejection and ELISA detected anti-HLA class I IgG (p=0.0004) and IgG1 (p<0.0001) was observed. The predictive value for IgG was 64% and for IgG1, 73% demonstrating that ELISA results allow the identification of patients at high risk to reject the transplanted kidney. Presence of anti-HLA class I IgG2, IgG3, IgG4 or IgM was not predictive of first year rejection episodes.
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© 1996 Plenum Press, New york
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Regan, J. et al. (1996). Correlation of Elisa-Measured anti-HLA Class I IGG and IGG1 Antibodies and First-Year Rejection Episodes. In: Abraham, N.G., Asano, S., Brittinger, G., Maestroni, G.J.M., Shadduck, R.K. (eds) Molecular Biology of Hematopoiesis 5. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0391-6_14
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DOI: https://doi.org/10.1007/978-1-4613-0391-6_14
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