NMR Structural Studies of Flexible Molecules
In recent years, NMR has been applied with great success to determine precise three- dimensional solution structures of compact globular proteins and small, inflexible cyclic peptides. NMR also holds promise for providing structural information on flexible biomolecules, although a quantitative description of structure is complicated by the inevitable population-weighted averaging of the key NMR parameters. Here we describe simple procedures for calculation of the dominant structure in the conformational ensemble of a linear peptide and for characterizing the domain motions and overall structural preferences of multidomain proteins.
KeywordsZinc Anisotropy Proline Polypeptide Macromolecule
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