Urinary Excretion of N1-Menthyl-2-Pyridone-5-Carboxylic Acid and the Fate of remaining of Trigonellie

  • S. Yuyama
  • Y. Kawano
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 398)

Abstract

During the development of a procedure for the determination of trigonelline (Tg) in human urine, an organic acid with a similar ultraviolet spectrum was isolated. The compound was found to be N1-methyl-2-pyridone-5-carboxylic acid (Tg-2Py) (Lindenblad et al. 1956), which Holman et al. (1950) were unable to detected in normal human urine. Tg-2Py was determined by using high performance thin-layer chromatography (HPTLC), and the level of Tg-2Py excretion was about 1.5 times of Tg or N1-methylnicotinamide(MNA) in 9 normal human urine (Yuyama et al, 1991). When Tg was orally administered in 3 human subjects, only 20% of the dose recovered in 24hr urine as unchanged Tg, and about 10% of the dose was converted into Tg-2Py (Yuyama et al. 1991). To explain the fate of missing Tg (60–70%),the possible mechanism for metabolism of Tg are as follows; 1) Tg may be metabolized further, expect Tg-2Py, 2) the ring fission could occur to pyridinium compounds, 3) Tg may be retained in the organ(s).

Keywords

HPLC Glycine Carboxyl Pyridinium Ketone 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Reference

  1. Lindenblad G.E., Kaihara M. and Price J.M., 1956, The occurrence of N-methyl-2-pyridone-5-carboxylic acid and its glycine conjugate in normal human urine../ Biol. Chem., 219: 893.Google Scholar
  2. Holman W.I.M, and Lange D.J., 1950, Metabolism of nicotinic acid and related compounds by humans. Nature. 165: 604.CrossRefGoogle Scholar
  3. Yuyama, S. and Suzuki, T.,1989, Bull. Inst. Public Health 38: 11.Google Scholar
  4. Yuyama S, and Suzuki T., 1991, The excretion of N1-methyl-2-pyridone-5-carboxylic acid and related compounds in human subjects after oral administration of nicotinic acid, trigonelline and N1-methyl- 2-pyridone-5-carboxylic acid. Adv. Exp. Med. Biol., 294: 475.Google Scholar

Copyright information

© Plenum Press, New York 1996

Authors and Affiliations

  • S. Yuyama
    • 1
  • Y. Kawano
    • 1
  1. 1.Department of Nutrition and BiochemistyThe Institute of Public Health TokyoJapan

Personalised recommendations