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On a Lumped Heat Transfer Model of Freezing Process of Cryopreservation Media

  • D. Y. Gao
  • J. Liu
  • F. Kleinhans
  • A. Ungan
  • J. K. Critser
Part of the A Cryogenic Engineering Conference Publication book series (ACRE, volume 41)

Abstract

In cryopreservation, biological cells are usually suspended in and cooled together with a cryopreservation medium. A lumped heat transfer model and an apparent specific heat form of energy equation were developed to formulate the cooling process of the cryopreservation media. Using glycerol as a model cryopreotective agent, the freezing process of ternary cryopreservation media (glycerol-NaCl-water) in cylindrical plastic straws were studied numerically by computer simulation. Effects of four important factors on the temperature history in the media during the cooling process were investigated. These factors include: glycerol concentration, supercooling temperature of the media, ambient constant cooling rates, and radius of the straw. Results from computer simulation showed that these factors significantly influenced the thermal history in the ternary media, especially during the water phase transition period. It was also revealed that actual cooling rate in the media varied as a function of time and the maximum rate was much higher than the ambient constant cooling rate.

Keywords

Cool Rate Temperature History Glycerol Concentration Freezing Process Cryoprotective Agent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1996

Authors and Affiliations

  • D. Y. Gao
    • 1
    • 2
  • J. Liu
    • 1
    • 3
  • F. Kleinhans
    • 1
    • 3
  • A. Ungan
    • 2
  • J. K. Critser
    • 1
    • 4
    • 5
    • 6
    • 7
  1. 1.Cryobiology Research InstituteMethodist Hospital of IndianaIndianapolisUSA
  2. 2.Department of Mechanical EngineeringPurdue UniversityIndianapolisUSA
  3. 3.Department of PhysicsIndiana University-Purdue UniversityIndianapolisUSA
  4. 4.Department of Physiology & BiophysicsIndiana University School of MedicineIndianapolisUSA
  5. 5.Department of ObstetricsIndiana University School of MedicineIndianapolisUSA
  6. 6.Department of GynecologyIndiana University School of MedicineIndianapolisUSA
  7. 7.Department of Veterinary Clinical SciencesPurdue University School of Veterinary MedicineWest LafayetteUSA

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