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Natural Toxins 2 pp 481–498Cite as

Detection And Identification of Clostridium botulinum Neurotoxins

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 391))

Abstract

Botulism is a serious neuroparalytic illness that affects humans and various domestic and wild animal and avian species. It is due to the neurotoxic effect of a toxin produced by the anaerobic bacterium Clostridium botulinum. Botulism is most commonly known as a foodborne intoxication of humans; it also can result from growth of the toxigenic organism in a wound or, in the case of infant botulism, from colonization of the intestinal tract.

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References

  1. Aureli, P., Fenicia, L., Pasolini, B., Gianfranceschi, M., McCroskey, L.M., and Hatheway, C.L., 1986, Two cases of type E infant botulism caused by neurotoxigenic Clostridium butyricum in Italy, J. Infect. Dis. 154: 201 – 206.

    Google Scholar 

  2. Bluestone, B.I., Craig, M., Slovacek, R., Stundtner, L., Urciuoli, C, Walczak, I., and Luderer, A., Evanescent wave immunosensors for clinical diagnosis, in: “Biosensors with Fiberoptics,” Wise, D.L., and Wingard, L.B., Jr., eds., Humana Press, Clifton, New Jersey (1991).

    Google Scholar 

  3. Boroff, D.A., and Fleck, U., 1966, Statistical analysis of a rapid in vitro method for the titration of the toxin of Clostridium botulinum, J. Bacteriol. 92: 1580 – 1581.

    PubMed  CAS  Google Scholar 

  4. Boroff, D.A., and Shu-Chen, G., 1973, Radio-immunoassay for type A toxin of Clostridium botulinum, Appl. Microbiol 25: 545 – 549.

    CAS  Google Scholar 

  5. Bowmer, E.J., 1963, Preparation and assay of the International Standards for Clostridium botulinum types A, B, C, D and E antitoxins, Bull. Wld. Hlth. Org. 29: 701 – 709.

    CAS  Google Scholar 

  6. Campbell, K.D., Collins, M.D., and East, A.K., 1993, Gene probes for identification of the botulinal neurotoxin gene and specific identification of neurotoxin types B, E, and F, J. Clin. Microbiol. 31: 2255 – 2262.

    PubMed  CAS  Google Scholar 

  7. Cardella, M.A., Botulinum Toxoids, in: “Botulism: Proceedings of a Symposium,” Lewis, K.H., and Cassel, K., Jr., eds., U.S. Department of Health, Education, and Welfare, Public Health Service (PHS Pubi. No 999 FP-1), Cincinnati, Ohio (1964).

    Google Scholar 

  8. Dezfulian, M., Hatheway, C.L., Yolken, R.H., and Bartlett, J.G., 1984, Enzyme-linked immunosorbent assay for detection of Clostridium botulinum type A and type B toxins in stool samples from infants with botulism, J. Clin. Microbiol. 20: 379 – 383.

    PubMed  CAS  Google Scholar 

  9. Doellgast, G.J., Beard, G.A., Bottoms, J.D., Cheng, T., Roh, B.H., Roman, M.G., Hall, P.A., and Triscott, M.X., 1994, Enzyme-linked immunosorbent assay and enzyme-linked coagulation assay for detection of Clostridium botulinum neurotoxins A, B, and E and solution-phase complexes with dual-label antibodies, J. Clin. Microbiol. 32: 105 – 111.

    CAS  Google Scholar 

  10. Doellgast, G.J., Triscott, M.X., Beard, G.A., Bottoms, J.B., Cheng, T., Roh, B.H., Roman, M.G., Hall, P.A., and Brown, J.E., 1993, Sensitive enzyme-linked immunosorbent assay for detection of CLostridium botulinum neurotoxins A, B, and E using signal amplification via enzyme-linked coagulation assay, J. Clin. Microbiol. 31: 2402 – 2409.

    CAS  Google Scholar 

  11. Doellgast, G.J., Triscott, M.X., Beard, G.A., and Bottoms, J.D., 1994, Enzyme-linked immunosorbent assay — enzyme-linked coagulation assay for detection of antibodies to Clostridium botulinum neurotoxins A, B, and E and solution-phase complexes, J. Clin. Microbiol. 32: 851 – 853.

    CAS  Google Scholar 

  12. Evancho, G.M., Ashton, D.H., and Briskey, E.J., 1973, A standardized reversed passive hemagglutination technique for the determination of botulinum toxin, J. Food Sci. 38: 764 – 767.

    Article  Google Scholar 

  13. Fach, P., Gibert, M., Griffais, R., Guillou, J.P., and Popoff, M.R., 1995, PCR and gene probe identification of botulinum neurotoxin A- B- E- F- and G-producing Clostridium spp. and evaluation in food samples, Appl. Environ. Microbiol. 61: 389 – 392.

    CAS  Google Scholar 

  14. Ferreira, J.L., Baumstark, B.R., Hamdy, M.K., and McCay, S.G., 1993, Polymerase chain reaction for the detection of Clostridium botulinum type A in foods, J. Food Protect. 56: 18 – 20.

    CAS  Google Scholar 

  15. Ferreira, J.L., and Hamdy, M.K., 1995, Detection of botulinal toxin genes: types A and E or B and F using the multiplex polymerase chain reaction, J Rapid Meth. Automat. Microbiol. 3: 177 – 183.

    CAS  Google Scholar 

  16. Ferreira, J.L., Hamdy, M.K., Herd, A.L., McCay, S.G., and Zapatka, F.A., 1987, Monoclonal antibody for the detection of Clostridium botulinum type A toxin, Mol. Cell. Probes 1: 337 – 345.

    Article  CAS  Google Scholar 

  17. Ferreira, J.L., Hamdy, M.K., McCay, S.G., and Baumstark, B.R., 1992, An improved assay for identification of type A Clostridium botulinum using the polymerase chain reaction, J Rapid Meth. Automat. Microbiol. 1: 29 – 39.

    Article  CAS  Google Scholar 

  18. Ferreira, J.L., Hamdy, M.K., McCay, S.G., Hemphill, M., Kirma, N., and Baumstark, B.R., 1994, Detection of Clostridium botulinum type F using the polymerase chain reaction, Mol. Cell. Probes 8: 365 – 373.

    Article  CAS  Google Scholar 

  19. Ferreira, J.L., Hamdy, M.K., McCay, S.G., and Zapatka, F.A., 1990, Monoclonal antibody to type F Clostridium botulinum toxin, Appl. Environ. Microbiol. 56: 808 – 811.

    CAS  Google Scholar 

  20. Ferreira, J.L., Hamdy, M.K., Zapatka, F.A., and Hebert, W.O., 1981, Immunodiffusion method for detection of Clostridium botulinum type A, Appl. Environ. Microbiol. 42: 1057 – 1061.

    CAS  Google Scholar 

  21. Ferreira, J.L., Hamdy, M.K., Zapatka, F.A., and Hebert, W.O., 1983, Immunodiffusion method for detection of Clostridium botulinum types A, B, E and F, J. Food Safe. 5: 87 – 94.

    Article  Google Scholar 

  22. Ferreira, J.L., Hatheway, C.L., Johnson, E.A., and Collins, M.D., Unpublished collaborative studies on neurotoxin genes of Clostridium botulinum.

    Google Scholar 

  23. Franciosa, G., Ferreira, J.L., and Hatheway, C.L., 1994, Detection of type A, B, and E botulism neurotoxin genes in Clostridium botulinum and other Clostridium species by PCR: evidence of unexpressed type B toxin genes in type A toxigenic organisms, J. Clin. Microbiol. 32: 1911 – 1917.

    CAS  Google Scholar 

  24. Gibson, A.M., Modi, N.K., Roberts, T.A., Hambleton, P., and Meiling, J., 1988, Evaluation of a monoclonal antibody-based immunoassay for detecting type B Clostridium botulinum toxin produced in pure culture and an inoculated model cured meat system, J. Appl. Bacteriol. 64: 285 – 291.

    PubMed  CAS  Google Scholar 

  25. Gibson, A.M., Modi, N.K., Roberts, T.A., and Shone, C.C., 1987, Evaluation of a monoclonal antibody-based immunoassay for detecting type A Clostridium botulinum toxin produced in pure culture and an inoculated model cured meat system, J. Appl. Bacteriol. 63: 217 – 226.

    PubMed  CAS  Google Scholar 

  26. Glasby, C, and Hatheway, C.L., 1983, Fluorescent-antibody reagents for the identification of Clostridium botulinum, J. Clin. Microbiol. 18: 1378 – 1383.

    PubMed  CAS  Google Scholar 

  27. Glasby, C, and Hatheway, C.L., 1984, Evaluation of fluorescent-antibody tests as a means of confirming infant botulism, J. Clin. Microbiol. 20: 1209 – 1212.

    PubMed  CAS  Google Scholar 

  28. Goodnough, M.C., Hammer, B., Sugiyama, H., and Johnson, E.A., 1993, Colony immunoblot assay of botulinal toxin, Appl. Environ. Microbiol. 59: 2339 – 2342.

    CAS  Google Scholar 

  29. Habermann, E., and Bernath, S., 1975, Preparation, measurement and possible use of human antitoxin against Cl. botulinum A, B, and E toxins, Med. Microbiol. Immunol. 161: 203 – 210.

    Article  CAS  Google Scholar 

  30. Hall, J.D., McCroskey, L.M., Pincomb, B.J., and Hatheway, C.L., 1985, Isolation of an organism resembling Clostridium barati which produces type F botulinal toxin from an infant with botulism, J. Clin. Microbiol. 21: 654 – 655.

    PubMed  CAS  Google Scholar 

  31. Hatheway, C.L., Botulism, in: “Laboratory Diagnosis of Infectious Diseases: Principles and Practice,” Balows, A., Hausler, W.H., Jr., Ohashi, M., and Turano, A., eds., Springer-Verlag, New York (1988).

    Google Scholar 

  32. Hatheway, C.L., 1990, Toxigenic clostridia, Clin. Microbiol. Rev. 3: 67 – 98.

    Google Scholar 

  33. Hatheway, C.L., and Dang, C, Immunogenicity of the neurotoxins of Clostridium botulinum, in: “Therapy with botulinum toxin,” Jankovic, J., and Hallett, M., eds., Marcel Dekker, Inc., New York (1994).

    Google Scholar 

  34. Hatheway, C.L., Ferreira, M.C., and McCroskey, L.M., Evaluation of various factors in the mouse toxicity test for identification of botulinal toxin. Abstr., Ann. Mtg., Am. Soc. Microbiol., Las Vegas, 1985.

    Google Scholar 

  35. Horiguchi, Y, Kozaki, S., and Sakaguchi, G., 1984, Determination of Clostridium botulinum toxin by reversed passive latex agglutination, Jpn. J. Vet. Sci. 46: 487 – 491.

    Article  CAS  Google Scholar 

  36. Huhtanen, C.N., Whiting, R.C., Miller, A.J., and Call, J.E., 1992, Qualitative correlation of the mouse neurotoxin and enzyme-linked immunoassay for determining Clostridium botulinum types A and B toxins, J. Food Protect. 12: 119 – 127.

    CAS  Google Scholar 

  37. Inukai, Y, and Riemann, H., 1968, Detection of intracellular botulinum E toxin by fluorescent antibody technique, Jap. J. Vet. Res. 16: 39 – 43.

    CAS  Google Scholar 

  38. Johnson, H.M., Brenner, K., Angelotti, R., and Hall, H.E., 1966, Serological studies of types A, B, and E botulinal toxins by passive hemagglutination and bentonite flocculation, J. Bacteriol. 91: 967 – 973.

    PubMed  CAS  Google Scholar 

  39. Kautter, D.A., Lynt, R.K., and Solomon, H.M., Clostridium botulinum, in: “Bacteriological Analytical Manual,” U.S. Food and Drug Administration, Washington, D.C. (1984).

    Google Scholar 

  40. Kautter, D.A., and Solomon, H.M., 1977, Collaborative study of a method for the detection of Clostridium botulinum and its toxins in foods, J. Assoc. Off. Anal. Chem. 60: 541 – 545.

    PubMed  CAS  Google Scholar 

  41. Kempner, W., 1897, Weiterer Beitrag zur Lehre von der Fleischvergiftung. Das Antitoxin des Botulismus, Ztschr. Hyg. Infektionskh. 26: 481 – 500.

    Article  Google Scholar 

  42. Kozaki, S., Dufrenne, J., Hagenaars, A.M., and Notermans, S., 1979, Enzyme-linked immunosorbent assay (ELISA) for detection of Clostridium botulinum type B toxin, Jpn. J. Med. Sci. Biol. 32: 199 – 205.

    PubMed  CAS  Google Scholar 

  43. Leuchs, J., 1910, Beitraege zur Kenntnis des Toxins und Antitoxins des Bacillus botulinus, Ztschr. Hyg. Infektskh. 65: 55 – 84.

    Google Scholar 

  44. Lewis, G.E.J., Kulinski, S.S., Reichard, D.W., and Metzger, J.F., 1981, detection of Clostridium botulinum type G toxin by enzyme-linked immunosorbent assay, Appl. Environ. Microbiol. 42:1018–1022.

    Google Scholar 

  45. Lüthgen, W., 1972, Eine modifikation des Mäusetestes zum Nachweis der Botulismus C Intoxikation des Geflügels, Berl. Münch. Tierarzt. Wochenschr. 85: 107 – 110.

    Google Scholar 

  46. Mestrandrea, L.W., 1974, Rapid detection of Clostridium botulinum toxin by capillary tube diffusion, Appl. Microbiol. 27: 1017 – 1023.

    CAS  Google Scholar 

  47. Michalik, M., Grzybowski, J., Ligieza, J., and Reiss, J., 1986, Enzyme-linked immunosorbent assay (ELISA) for the detection and differentiation of Clostridium botulinum toxins type A and B, J. Immunol. Meth. 93: 225 – 230.

    Article  CAS  Google Scholar 

  48. Notermans, S., Dufrenne, J., and Kozaki, S., 1979, Enzyme-linked immunosorbent assay for detection of Clostridium botulinum type E toxin, Appl. Environ. Microbiol. 37: 1173 – 1175.

    CAS  Google Scholar 

  49. Notermans, S., Dufrenne. J., and Kozaki, S., 1982, The relation between toxicity and toxin-related-antigen contents of Clostridium botulinum types C and D cultures as determined by mouse bioassay and ELISA, Jpn. J. Med. Sci. Biol. 35: 203 – 211.

    PubMed  CAS  Google Scholar 

  50. Notermans, S., DuFrenne, J., and Van Schothorst. M., 1978, Enzyme-linked immunosorbent assay for detection of Clostridium botulinum toxin type A, Jpn. J. Med. Sci. Biol. 31: 81 – 85.

    PubMed  CAS  Google Scholar 

  51. Notermans, S.. Hagenaars, A.M.. and Kozaki, S., 1982, The enzyme-linked immunosorbent assay (ELISA) for the detection and determination of Clostridium botulinum toxins A, B, and E, Methods Enzymol. 84: 223 – 238.

    Article  PubMed  CAS  Google Scholar 

  52. Notermans, S., and Nagel, J., Assays for botulinum and tetanus toxins, in: “Botulinum Neurotoxin and Tetanus Toxin,” Simpson, L.L., ed., Academic Press, Inc., San Diego (1989).

    Google Scholar 

  53. Ogert, R.A., Brown. J.E., Singh, B.R., Shriver-Lake, L.C., and Ligler, F.S., 1992, Detection of Clostridium botulinum toxin A using a fiber optic-based biosensor, Anal. Biochem. 205: 306 – 312.

    Article  PubMed  CAS  Google Scholar 

  54. Pearce, L.B., Borodic, G.E., First, E.R., and MacCallum, R.D., 1994, Measurement of botulinum toxin activity: evaluation of the lethality assay, Toxicol. Appl. Pharmacol. 128: 69 – 77.

    CAS  Google Scholar 

  55. Potter, M.D., Meng, J., and Kimsey, P., 1993, An ELISA for detection of botulinal toxin types A. B, and E in inoculated food samples, J. Food Protect. 56: 856 – 861.

    CAS  Google Scholar 

  56. Prevot, A.R., 1953, Rapport d’introduction du President du Sous-Comite Clostridium pour l’unification de la nomenclature des types toxigeniques de C botulinum, Int. Bull. Bacteriol. Nomenclature 3: 120 – 123.

    Article  Google Scholar 

  57. Ramon, G., 1924, Sur la toxine et sur l’anatoxine diphtheriques; pourvoir floculant et propriétés immunisantes, Ann. Inst. Pasteur 38: 1 – 10.

    CAS  Google Scholar 

  58. Ransom, G.M., Lee, W.H., Elliot, E.L., and Lattuada, C.P., Enzyme-linked immunosorbent assays (ELISAs) to detect botulinum toxins using high titer rabbit antisera, in: “Botulinum and Tetanus Neurotoxins,” DasGupta, B.R., ed., Plenum Press, New York (1993).

    Google Scholar 

  59. Rubin, L.G., Dezfulian, M., and Yolkin, R.H., 1982, Serum antibody response to Clostridium botulinum toxin in infant botulism, J. Clin. Microbiol. 16: 770 – 771.

    PubMed  CAS  Google Scholar 

  60. Sakaguchi, G., 1983, Clostridium botulinum toxins, Pharmacol. Ther. 19: 165 – 194.

    CAS  Google Scholar 

  61. Sakaguchi, G., and Sakaguchi, S., 1968, Rapid bioassay for Clostridium botulinum type E toxins by intravenous injection into mice, Jpn. J. Med. Sci. Biol. 21: 369 – 378.

    PubMed  CAS  Google Scholar 

  62. Sakaguchi, G., Sakaguchi, S., Kozaki, S.. Sugii, S.. and Ohishi. I., 1974, Cross reaction in reversed passive hemagglutination between Clostridium botulinum type A and B toxins and its avoidance by the use of antitoxic component immunoglobulin isolated by affinity chromatography, Jpn. J. Med. Sci. Biol. 27: 161 – 172.

    PubMed  CAS  Google Scholar 

  63. Schantz, E.J., and Kautter, D.A., 1978, Standardized assay for Clostridium botulinum toxins, J. Assoc. Off. Anal. Chem. 61: 96 – 99.

    CAS  Google Scholar 

  64. Shone, C, Wilton-Smith, P., Appleton, N., Hambleton, P., Modi, N., Gatley, S., and Meiling, J., 1985, Monoclonal antibody-based immunoassay for type A Clostridium botulinum toxin is comparable to the mouse bioassay, Appl. Environ. Microbiol. 50: 63 – 67.

    CAS  Google Scholar 

  65. Siegel, L.S., 1988, Human response to botulinum pentavalent (ABCDE) toxoid determined by a neutralization test and by an enzyme-linked immunosorbent assay, J. Clin. Microbiol. 26: 2351 – 2356.

    PubMed  CAS  Google Scholar 

  66. Smith, C, and Hamdy, M.K.. 1992, Immunoenzyme for rapid screening of Clostridium botulinum type A cells, J Rapid Meth. Automat. Microbiol. 1: 149 – 163.

    Article  Google Scholar 

  67. Suen, J.C, Hatheway, C.L., Steigerwalt, A.G., and Brenner, D.J., 1988, Clostridium argentinense, sp. nov: a genetically homogenous group composed of all strains of Clostridium botulinum toxin type G and some nontoxigenic strains previously identified as Clostridium subterminale or Clostridium hastiforme. Int. J. Syst. Bacteriol. 38: 375 – 381.

    Article  Google Scholar 

  68. Sugiyama, H., Brenner, S.L., and DasGupta, B.R., 1975, Detection of Clostridium botulinum toxin by local paralysis elicited with intramuscular challenge, Appl. Microbiol. 30: 420 – 423.

    CAS  Google Scholar 

  69. Szabo, E.A., Pemberton, J.M., and Desmarchelier, P.M., 1992, Specific detection of Clostridium botulinum type B by using the polymerase chain reaction, Appl. Environ. Microbiol. 58: 418 – 420.

    CAS  Google Scholar 

  70. Szabo, E.A., Pemberton, J.M., and DesMarchelier, P.M., 1993, Detection of the genes encoding botulinum neurotoxin types A to E by the polymerase chain reaction, Appl. Environ. Microbiol. 59: 3011 – 3020.

    CAS  Google Scholar 

  71. Szabo, E.A., Pemberton, J.M., Gibson, A.M., Eyles, M.J., and Desmarchelier, P.M.. 1994, Polymerase chain reaction for detection of Clostridium botulinum types A, B, and E in food, soil and infant feces, J. Appl. Bacteriol. 76: 539 – 545.

    CAS  Google Scholar 

  72. Szabo, E.A., Pemberton, J.M., Gibson, A.M., Thomas, R.J., Pascoe, R.R., and Desmarchelier, P.M., 1994, Application of PCR to a clinical and environmental investigation of a case of equine botulism, J. Clin. Microbiol. 32: 1986 – 1991.

    PubMed  CAS  Google Scholar 

  73. Van Ermengem, E., 1897, Ueber einen neuen anaeroben Bacillus and seine Beziehungen zum Botulismus, Ztschr. Hyg. Infektionskh. 26: 1 – 56.

    Article  Google Scholar 

  74. Vermilyea, B., Walker, H.W, and Ayers, J.C, 1968, Detection of botulinal toxins by immunodiffusion, Appl. Microbiol. 16: 21 – 24.

    CAS  Google Scholar 

  75. Wernars, K., and Notermans, S., Gene probes for detection of food-borne pathogens, in: “Gene probes for bacteria”, Macario, A.J.L., and de Macario, E.C., eds., Academic Press, Inc., San Diego (1990).

    Google Scholar 

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Authors and Affiliations

  1. Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, National Center for Infectious Diseases, 1600 Clifton Road NE, Atlanta, Georgia, 30333, USA

    Charles L. Hatheway

  2. US Food and Drug Administration, Southeastern Regional Laboratories, 60 – 8th Street, NE, Atlanta, Georgia, 30309, USA

    Joseph L. Ferreira

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  1. Charles L. Hatheway
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  1. University of Massachusetts, Dartmouth, North Dartmouth, Massachusetts, USA

    Bal Ram Singh

  2. Colorado State University, Fort Collins, Colorado, USA

    Anthony T. Tu

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Hatheway, C.L., Ferreira, J.L. (1996). Detection And Identification of Clostridium botulinum Neurotoxins. In: Singh, B.R., Tu, A.T. (eds) Natural Toxins 2. Advances in Experimental Medicine and Biology, vol 391. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0361-9_39

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