Abstract
Recent development in biological mass spectrometry has contributed immensely to the growth in diverse fields such as molecular biology, biochemistry, pharmacology, toxinology, immunology and biotechnology.1–11 The mass spectrometric techniques provide versatility in ionization techniques and modes of detection along with femtomolar sensitivity, accuracy and reproducibility. This has enabled solving assorted complex structures of biopolymers with ease.1–9 In general, the mass spectrometric techniques have been demonstrated to be powerful tools for the characterization and detection of larger as well as smaller chemical and biological molecules.1–19
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Matsuo, T., Caprioli, R. M., Gross, M.L., and Seyama, Y., Eds., 1994, In Mass Spectrometry, Present and Future; Wiley, New York.
2. Burlingame, A.L., Boyd, R.K., and Gaskell, S.J., 1994, In Mass Spectrometry, Anal. Chem., 66, 634R–683R.
Hunt, D.F., Shabanowitz, J., Michel, H., and Engelhart, V.H., 1993, In Methods in Protein Sequence Analysis; Imhori, K., Saklyama, F., Eds., Plenum Press, New York, pp 127 – 133
Fenselau, C, Ed., 1994, In Mass Spectrometry for the Characterization to Microorganisms; ACS Symposium Series 541; American Chemical Society, Washington, D.C.
Desiderio, D.M., Ed., 1994, In Mass Spectrometry: Clinical and Biomedical Applications, Plenum Press, New York, Vol., 2.
Cotter, R.J., Ed., 1994, In Time of Flight Mass Spectrometry, ACS Symposium Series, 549, American Chemical Society, Washington, D.C.
7. Niessen, W.M. A., van der Greef, J., 1992, In Liquid Chromatography-Mass Spectrometry, Mercel Dekker, New York.
Settineri, CA., Burlingame, A.L., 1995, In Carbohydrate Analysis: High Performance Liquid Chromatography and Capillary Electrophoresis, El Rassi, Z., Ed., Elsevier Science Publishers, Amsterdam, The Netherlands.
Emary, W.B., Hand, O.W., Cooks, R.G., 1995, In Lasers and Mass spectrometry, Lubman, D.M., Ed., Oxford University Press, New York, 1990
McCloskey, J.A., Ed., 1990 Mass Spectrometry, Methods in Enzymology, Volume 193, Academic Press, New York
11. 1993, Journal of the American Society for Mass Spectrometry, Special Issue on Electrospray Ionization & Applications to Peptide & Protein Chemistry, Volume 4.
12. Cotter, R.J., 1992, In Time-of-flight Mass Spectrometry for the Structural Analysis of Biological Molecules, Anal.Chem., 64, 1027A–1039A
13. Cotter, R.J., 1991, In Matrix-Assisted Laser Mass Spectrometry of Biopolymers, Anal. Chem., 63, 1193A–1203A.
Huberty, M.C., Vath, J.E., Yu, W., Martin, S.A., 1993, Site-specific Carbohydrate Identification in Recombinant Proteins using MALDI-TOF MS, In Anal. Chem., 65, 2791 – 2800.
Pfeiffer,G., Dabrowski, U., Dabrowski, J., Strim, S., Strube, K.H., and Geyer, R., 1992, Carbohydrate Structure of a thrombin-like Serine Protease from Agkistrodon rhodostoma, In Eur. J. Biochem., 205, 961 – 978
Xiang, F., and Beavis, R.C, 1993, A Method to Increase contaminant Tolerance in Protein-Matrix-assisted Laser Desorption/Ionization by the Fabrication of Thin Protein-doped Polycrystalline films, In Rapid Commun. In Mass Spectrom., 199 – 204
Xhang, Y, Chen N., Wang, L., Yang, B., Qian X., and Ma L., 1994, Separation and identification of Proteins obtained from Agkistrodon acutus snake Venom by Capillary Zone electrophoresis and laser Desorption/Ionization Mass Monitoring, Biomedical chromatography, 8, 148 – 150.
Covey T, Huang, E.C., and Henion, J.D., 1991, Stuctural Characterization of Protein Tryptic Peptides via Liquid Chromatography/Mass spectrometry and Collision-Induced Dissociation of Their Doubly charged Molecular Ions, In Anal. Chem., 63, 1193 – 1200.
Kenny, P.T., and Orlando, R., 1992, Tandem mass Spectrometric Analysis of Peptides at the Femtomole Level, in Anal. Chem., 64, 957 – 960.
20. Caprioli, R.M., 1990, In Continuous-Flow fast Atom Bomardment Mass Spectrometry, John Wiley, New York.
Fenn, J.B., Mann, M., Meng, C.K., Wong, S.F., and Whitehouse, CM., 1990, Electrospray Ionization -Principles and Practice, In Mass Spectrom. Rev, 9, 37 – 70
Fabris, D., Kelly, M., Murphy, C, Wu, Z., and Fenselau, C, 1993, High-Energy Collision-Induced Dissociation of Multiply Charged Polypeptides Produced by electrospray, In J. Amer. Soc. Mass Spectrom., 4, 652 – 661
McLafferty, Ed., 1983, In Tandem Mass Spectrometry, John Wiley, New York
Biemann, K., 1990, Sequencing of Peptides by Mass Spectrometry and High Energy Collision-Induced Dissociation, In Ref 10, pp 455 – 479.
Krishnamurthy, T, Hauer, CR., Prabhakaran, M., Freedy, J.G., and Hayashi, K., 1994, Identification of Disulfide Bridges in a Cardiotoxic Peptide by Electrospray Ionization, In Biol. Mass Spectrom., 23, 719 – 726.
26. Krishnamurthy, T. Szafraniec, L., Hunt, D.F., Shabanowitz, J., Yates, III, J.R., Hauer, C.R., Carmichael, W.W., Skulberg, O., Codd, G.A., and Missler, S., 1989, in Structural Characterization of Toxic Cyclic Peptides from Blue-green Algae by Tandem Mass Spectrometry, In Proc. Natl. Acad. Sci. USA, 86, 770–774.
Kaiser, I.I., Griffin, P.R., Aird, S.D., Hudiburg, S., Shabanowitz, J., Francis, B., John, T.R., Hunt, D.F., and Odell, G.V, 1994, Primary Structures of Proteins from the Venom of Mexican Red Knee Terantula, In Toxicon, 32, 1083 – 93
Ren, K., Bannon, J.D., Pancholi, V., Cheung, A.L., Robbins, J.C, Fischetti, V.A., and Zabriskie, J.B., 1994, Characterization and Biological Properties of a New Staphylococcal Exotoxin, In J. Exp. Med., 180, 1675 – 83.
Slos, P., Speck, D., Accart, N., Kolbe, H.V., Schubnel, D., Bouchon, B., Bischoff, R., and Kieny, M.P., 1994, Recombinant Cholera Toxin B Subunit in E. Coli: High-level Secretion, Purification, and Characterization, In Protein Expression Purif., 5, 518 – 26
Perkins, J.R., Tomer, K.B., 1994, Capilllary Electrophoresis/Electrospray Mass Spectrometry using a High-Performance magnetic Secttor Mass Spectrometer, In Anal. Chem., 66, 2835 – 40
Monks, S.A., Gould, A.R., Lumley, P.E., Alewood, P.F., Kerm, W.R., Goss, N.H., and Norton, R.S., 1994, Limited Proteolysis Study of Structure-function Relationships in ShI, a Polypeptide Neurotoxin from a Sea Anemone, In Biochim. Biophys. Acta, 1207, 93 – 101.
Garcia, M.L., Garcia-calvo, M., Hidalgo, P., Lee, A., and MacKinnon, R., 1994, Purification and Characterization of Three Inhibitors of Voltage-Dependent K+ Channels from Leiurus Quinquestriatus Va. Hebraeus Venom, In Biochemistry, 33, 6834 – 9.
Gowda, D.C, Jackson, C.M., Hensley, P., and Davidson, E.A., 1994, Facor X-activating glycoprotein of Russell’s Viper Venom Polypeptide Compositions and Characterization of the Carbohydrate Moieties, In J. Biol. Chem., 269, 10644 – 50.
Krishnamurthy, T., 1994, Chiralities in Microcystins, In J. Amer. Soc. For Mass Spectrom., 5, 724 – 30.
Dufton, M.J., and Hider, H.C., 1983, Conformational Properties of the Neurotoxins and Cytotoxins isolated from Elapid Snake Venoms, In CRC Crit.Rev. Biochem., 14, 113 – 171.
Sivonen, K., Namikoshi, M., Evans, W.E., Fardig, M., Carmichael, W.W., and Reinhart, K.L., 1992, Three New Microcystins, Cyclic Heptapeptide Hepatotoxins, from Nostoc. Sp Strain 152, In Chem.Res.Toxicol. 5, 464 – 469.
Namikoshi, M., Reinhart, K.L., Saka, R., Slotts, R.R., Dahlem, A.M., Beasley, V.R., Carmichael, W.W., and Evans, W.R., 1992, Identification of 12 Hepatotoxins from a Homer Lake Bloom of the Cyanobacteria Microcystis aeruginosa, Microcystis viridis, and Microcystis wesenbergii: Nine New Microcystins, In J.Org.Chem., 57, 866 – 872.
38. Carmichael, W.W., Krishnamurthy, T., Beasley, V.A., Min-Juan, Y., Bunner, D.L., Moore, J.E., Eloff, J.N., Reinhart, K.L., Falconer, L., Runnegar, M., Corham, P., Skulberg, O.M., Harada, K., and Watanabi, M., Naming of Cyclic Heptapetide Toxins of Cyanobacteria (Blue-green Algae), In Toxicon, 26, 971–73.
Chau, M.H., and Nelson, J.W., 1992, Cooperative Disulfide Bond Formation in Apamin, In Biochemistry, 31, 4445 – 4450.
Chiou, S., Raynor, R.L., Bin, Z., Chambers, T.C., and Kuo, J.F.,1993, Cobra Venom Cardiotoxin (Cytotoxin) Isoforms and Neurotoxin: Comparative Potency of Protein Kinase C Inhibitors and Cancer Cell Cytotoxicity and Modes of Enzyme Inhibition, In Biochemistry, 32, 2062.
Tu, A., Ed., 1977, In Venoms: Chemistry & Molecular Biology, Wiley Interscience, New York.
Aird, S.D., Kruggel, W.G., and Kaiser, I.I., 1990, Amino Acid Sequence of the Basic Subunit of Mojave Toxin from the Venom of the Mojave Rattlesnake (Crotalus scutulatus), In Toxicon, 28, 669 – 673.
Olivera, B.M., Rivier, J., Scott, J.K., Hillyard, D.R., and Cruz, L.J., 1991, Conotoxins, In J. Biol. chem., 266, 22067 – 22070.
Olivera, B.M., 1987, Neuronal Calcium channel Antagonists. Discrimination between Calcium Channel Subtypes using ω-Conotoxin from Conus magus Venom, In Biochemistry, 26, 2086 – 209.
Tu, A., Colarado State University, Fort Collins, CO, USA, Personal Communication.
Olivera, B.M., Rivier, R., Clark, C, Railo, C.A., Corpuz, G.P., Abogadie, Fe C, Mena, E.E., Woodward, S.R., Hillyard, D.R., and Cruz, L.J., Diversity of Conus neuropeptides, In Science, 249, 257–263.
Loo, J.A., Ogorzalek, R.R., and Andrewa, P.C., 1993, Primary to Quarternary Protein Structure Determination with Electrospray Ionization and Magnetic Sector Mass Spectrometry, In Org.Mass Spectrom., 28, 1640 – 1649.
Fisher, W.H., Rivier, J.E., and Craig, A.G., 1993, In Situ Reduction Suitable for Matrix-assisted Laser Desorption/Ionization and Liquid Secondary Ionization using Tris)2-carboxyethyl)phosphine, In Rapid Comm. In Mass Spectrom., 7, 225 – 228.
Roepstrorff, P., 1984, Proposal for a Comman Nomenclature for Sequence Ions in Mass Spectra of Peptides, Biomed. Mass Spectrom., 11, 601.
Botes, D.P., Tuinman, A.A., Wessels, P.L., Viljoen, C.C., Kruger, H., Williams, D.H., Santikarn, S., Smith, R.J., and Hammonds, S., 1984, The Structure of Cyanoginosin-LA, a Cyclic Heptapeptide Toxin from the Cyanobacterium Microsystis aeruginosa, In J. Chem. Soc. Perkin Trans., 2311 – 2318
Teshima, T, Wakamiya, T., Arakami, Y, Nakajima, T., kawai, N., and Shiba, T., 1987, Total synthesis of ADDA, the Unique C20 Amino Acid of Cyanobacterial Hepatotoxins, In Tetrahedron Letters, 29, 3509 – 3510
Muniz, Z.M., Pracej, D.N., and Dolly, J.O., 1992, Characterization of Monoclonal Antibodies against Voltage-Dependent K+ Channels Raised Using α-Dendrotoxin Acceptors Purified from Bovine Brain, In Biochemistry, 31, 12297 – 12303.
Sahara, Y., Robinson, H.P., Miwa, A., and Kawai, N., 1991, A Voltage-clamp Study of the Effects of Joro Spider Toxin and Zic on Excitatory synaptic Transmission in CA1 Pyramidal Cells of the Quinea Pig Hippocampal Slice, In Neurosci. Res., 10, 200 – 210.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Plenum Press, New York
About this chapter
Cite this chapter
Krishnamurthy, T., Prabhakaran, M., Long, S.R. (1996). Mass Spectrometric Investigations on Proteinaceous Toxins and Antibodies. In: Singh, B.R., Tu, A.T. (eds) Natural Toxins 2. Advances in Experimental Medicine and Biology, vol 391. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0361-9_37
Download citation
DOI: https://doi.org/10.1007/978-1-4613-0361-9_37
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-8016-0
Online ISBN: 978-1-4613-0361-9
eBook Packages: Springer Book Archive