Abstract
Mutant rat trypsin Asp189 Ser was prepared and complexed with highly purified human α1 -proteinase inhibitor. The complex formed was purified to homogeneity and studied by N-terminal amino acid sequence analysis and limited proteolysis with bovine trypsin. As compared to uncomplexed mutant trypsin the mutant enzyme complexed with α1 -proteinase inhibitor showed a highly increased susceptibility to enzymatic digestion. The peptide bond selectively attacked by bovine trypsin was identified as the Arg117 — Val118 one of trypsin. The structural and mechanistic relevance of this observation to serine proteinase-substrate and serine proteinase-serpin reactions are discussed.
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© 1996 Plenum Press, New York
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Kaslik, G., Patthy, A., Bálint, M., Gráf, L. (1996). Trypsin Complexed with α1-Proteinase Inhibitor Has an Increased Structural Flexibility. In: Zaidi, Z.H., Smith, D.L. (eds) Protein Structure — Function Relationship. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0359-6_9
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DOI: https://doi.org/10.1007/978-1-4613-0359-6_9
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