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Trypsin Complexed with α1-Proteinase Inhibitor Has an Increased Structural Flexibility

  • Gyula Kaslik
  • András Patthy
  • Miklós Bálint
  • László Gráf

Abstract

Mutant rat trypsin Asp189 Ser was prepared and complexed with highly purified human α1 -proteinase inhibitor. The complex formed was purified to homogeneity and studied by N-terminal amino acid sequence analysis and limited proteolysis with bovine trypsin. As compared to uncomplexed mutant trypsin the mutant enzyme complexed with α1 -proteinase inhibitor showed a highly increased susceptibility to enzymatic digestion. The peptide bond selectively attacked by bovine trypsin was identified as the Arg117 — Val118 one of trypsin. The structural and mechanistic relevance of this observation to serine proteinase-substrate and serine proteinase-serpin reactions are discussed.

Keywords

Limited Proteolysis Serine Proteinase Action Bovine Pancreatic Trypsin Inhibitor Bovine Trypsin Porcine Pancreatic Elastase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1996

Authors and Affiliations

  • Gyula Kaslik
    • 1
  • András Patthy
    • 2
  • Miklós Bálint
    • 1
  • László Gráf
    • 1
  1. 1.Department of BiochemistryEötvös UniversityBudapestHungary
  2. 2.Agricultural Biotechnology CenterGödöllõHungary

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