Novel Members of Mammalian Kexin Family Proteases, Pace 4C, Pace 4D, PC 7A and PC 7B
Biologically active peptides, such as peptide hormones, neuropeptides and growth factors, are known to be synthesized as larger precursor proteins and converted to active peptides by proteolytic cleavage at the carboxyl side of paired basic amino acids1,2,3. This process is found also in the activation of membrane-bound receptor proteins such as insulin receptor and many secreted proteins like serum albumin. Kexin family proteases were identified as processing proteases for these proteins; they share a subtilisin-like catalytic domain and require calcium ion for their activities2,3. Kexin family proteases are widely distributed in nature, from yeast, to mammals4,5,6. Currently seven mammalian kexins have been reported: furin4, PCI/37, PC2,8 PACE45,6, PC49, PC5/610, and PC7 11.
KeywordsHine eDNA Proinsulin
Unable to display preview. Download preview PDF.
- 8.Bennet, D.L., Bailyes, E.M., Nielsen, E., Guest, P.C., Rutherford, N.G., Arden, S. D. and Hutton J. C., 1992, Identification of the type 2 proinsulin processing endopeptidase as PC 2, a member of the eukaryote subtilisin family, J. Biol. Chem. 267: 15229–15236.Google Scholar
- 11.Tsuji, A., Hine, C, Mori, K., Tamai, Y, Higashine, K., Nagamune, H. and Matsuda, Y, 1994, A novel mem¬ber, PC 7, of the mammalian kexin-like protease family: Homology to PACE 4A, Its brain specific expression and identification of isoforms, Biochem. Biophys. Res. Commun., 202: 1452–1459.PubMedCrossRefGoogle Scholar
- Tsuji, A., Mori, K., Hine, C, Tamai, Y, Nagamune, H. and Matsuda, Y., 1994, The tissue distribution of mRNAs for the PACE 4 isoforms, Kexin-like processing protease: PACE 4C and PACE 4D are major transcripts among PACE 4 isoforms, Biochem. Biophys. Res. Commun., 202:1215–1221.PubMedCrossRefGoogle Scholar