Abstract
The geometry of the microvascular bed as well as its perfusion pattern are important determinants of oxygen transport to tissues. In the present study, we analyzed capillary geometry in the rat parietal cortex using discriminative morphometric methods. Specifically, we labeled arteriolar capillary (i.e. the proximal portion of the capillary bed, AC) and venular capillary (i.e. the distal portion of the capillary bed, VC) endothelium with different colors. While the geometry of the capillary bed sets the surface area for diffusion of oxygen, the pattern of perfusion within the capillary bed under in vivo conditions provides information as to regional conditions for oxygen diffusion. Accordingly, we used a highly concentrated colloidal gold solution as a plasma label, to identify capillaries perfused with plasma under controlled conditions. While gold particles may be directly visualized on electron micrographs, they require enhancement with silver to make them visible at the light microscopic level. To our knowledge, this is the first study where these methods have been combined within the same histological section, i.e. enzymatic labeling of the capillary endothelium as well as delineation of capillary perfusion with silver enhanced colloidal gold particles.
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Batra, S., Koyama, T., Gao, M., Horimoto, M., and Rakusan, K., 1992, Microvascular geometry of the rat heart: arteriolar and venular capillary regions, Jpn. Heart J. 33:817–828.
Batra, S., Kuo, C, and Rakusan, K., 1989, Spatial distribution of coronary capillaries: A-V segment staggering, Adv. Exp. Med. Biol. 248:241–247.
Batra, S. and Rakusan, K., 1991, Geometry of capillary networks in volume overloaded rat heart, Microvasc. Res. 42:39–50.
Buchweitz, E. and Weiss, H. R., 1986, Alterations in perfused capillary morphometry in awake vs. anesthetized brain, Brain Res. 377:105–111.
Buchweitz-Milton, E and Weiss, H. R., 1988, Perfused microvascular morphometry during middle cerebral artery occlusion, Am. J. Physiol. 255:H623–H628.
Francois-Dainville, E.,Buchweitz, E., and Weiss, H. R., 1986, Effect of hypoxia on percent of arteriolar and capillary beds perfused in the rat brain, J. Appi. Physiol. 60:280–288.
Göbel, U., Theilen, H., and Kuschinsky, W, 1990, Congruence of total and perfused capillary network in rat brains, Circ. Res. 66:271–281.
Hoppeler, H., Mathieu, O., Weibel, E. R., Krauer, R., Lindstedt, S. L., and Taylor, C. R.,1981, Design of the mammalian respiratory system. VIII. Capillaries in skeletal muscles, Respir. Physiol. 44:129–150.
Klein, B., Kuschinsky, W, Schröck, H., and Vetterlein, F., 1986, Interdepency of local capillary density, blood flow, and metabolism in rat brains, Am. J. Physiol. 251.H1333–H1340.
König, M. F., Lucocq, J. M., and Weibel, E. R., 1993, Demonstration of pulmonary vascular perfusion by electron and light microscopy, J. Appi. Physiol. 75:1–7.
Kuschinsky, W., Klein, B., Schröck, H. and Vetterlein, F.,1987, The lokal density of perfused capillaries in rat brain. In Stroke and Microcirculation. J. Cervos-Navarro and R. Ferszt, editors. Raven, New York. 167–170.
Lojda, Z., 1979, Studies on dipeptidyl (amino) peptidase IV (glycyl-proline naphthylamidase). II. Blood vessels, Histochem. 59:153–166.
Mermod, L., Hoppeler, H., Kayar, S. R., Straub, R., and Weibei, E. R., 1988, Variability of fiber size, capillary density and capillary length related to horse muscle fixation procedures, Acta Anat. 133:89–95.
Romanul, F. C. A. and Bannister, R. G., 1962, Localized areas of high alkaline phosphatase activity in the terminal arterial tree, J. Cell Biol. 15:73–84.
Slot, J. W. and Geuze, H. J., 1985, A new method of preparing gold probes for multiple-labeling cytochemistry, Eur. J. Cell Biol. 38:87–93.
Vetterlein, F., Demmerle, B., Bardosi, A., Göbel, U., and Schmidt, G., 1990, Determination of capillary perfusion pattern in rat brain by timed plasma labeling, Am. J. Physiol. 258:H80–H84.
Weibel, E.R, 1979, Stereological methods. Vol. 1. Academic, London.
Weiss, H. R., Buchweitz, E., Murtha, T. J., and Auletta, M., 1982, Quantitative regional determination of morphometric indices of the total and perfused network in the rat brain, Circ. Res. 51:494–503.
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© 1996 Plenum Press, New York
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Batra, S., König, M.F. (1996). A Novel Method to Demonstrate Capillary Geometry and Perfusion Patterns in Rat Brain from the Same Histological Section. In: Ince, C., Kesecioglu, J., Telci, L., Akpir, K. (eds) Oxygen Transport to Tissue XVII. Advances in Experimental Medicine and Biology, vol 388. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0333-6_9
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DOI: https://doi.org/10.1007/978-1-4613-0333-6_9
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