Is There a Reduction of Ischaemia/Reperfusion Damage in the Isolated Rat Heart by Alternating Oxygenated and Deoxygenated Reperfusion Solutions?
Recent pilot experiments (Osbakken et al, 1994) suggested that alternate perfusion with normoxic and anoxic solutions (‘N2/02-cycling’) might have beneficial haemodynamic effects in rat hearts that had developed high diastolic and low developed left ventricular pressures for which the cause was not precisely known. In these pilot experiments, in which the perfusate was recirculated, isolated rat hearts were perfused with Krebs-Henseleit solution according to Langendorff. Even though the hearts developed rigor or ventricular fibrillation, coronary flow was maintained throughout (up to 5 ml/min) with solution gassed with 95% 02—5% C02. Subsequently, arrested hearts recovered when perfused with solution gassed with 95% N2-5% C02, alternating with solution gassed with 95% 02—5% C02, whereas no recovery occurred when hearts were perfused with solution gassed with 95% 02—5% C02 alone. The mechanism of the putative beneficial action of N2/02-cycling is unknown, but it was hypothesized that the alternate gassing regimen might protect the heart from oxygen free radical damage.
KeywordsGlobal Ischaemia Creatine Phosphokinase Develop Left Ventricular Pressure Coronary Vascular Resistance Sudden Death Syndrome
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