Is There a Reduction of Ischaemia/Reperfusion Damage in the Isolated Rat Heart by Alternating Oxygenated and Deoxygenated Reperfusion Solutions?

  • D. R. Rutgers
  • J. H. G. M. van Beek
  • M. D. Osbakken
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 388)


Recent pilot experiments (Osbakken et al, 1994) suggested that alternate perfusion with normoxic and anoxic solutions (‘N2/02-cycling’) might have beneficial haemodynamic effects in rat hearts that had developed high diastolic and low developed left ventricular pressures for which the cause was not precisely known. In these pilot experiments, in which the perfusate was recirculated, isolated rat hearts were perfused with Krebs-Henseleit solution according to Langendorff. Even though the hearts developed rigor or ventricular fibrillation, coronary flow was maintained throughout (up to 5 ml/min) with solution gassed with 95% 02—5% C02. Subsequently, arrested hearts recovered when perfused with solution gassed with 95% N2-5% C02, alternating with solution gassed with 95% 02—5% C02, whereas no recovery occurred when hearts were perfused with solution gassed with 95% 02—5% C02 alone. The mechanism of the putative beneficial action of N2/02-cycling is unknown, but it was hypothesized that the alternate gassing regimen might protect the heart from oxygen free radical damage.


Global Ischaemia Creatine Phosphokinase Develop Left Ventricular Pressure Coronary Vascular Resistance Sudden Death Syndrome 
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  1. van Beek J.H.G.M., Loiselle D.S. and Westerhof N., 1992, Calculation of oxygen diffusion across the surface of isolated perfused hearts, Am. J. Physiol. 263;H1003–H1010.PubMedGoogle Scholar
  2. van Beek J.H.G.M., Osbakken M.D. and Chance B., This volume, Measurement of the oxygenation status of the isolated perfused rat heart using near infrared detection.Google Scholar
  3. Benzi R.H. and Lerch R., 1992, Dissociation between contractile function and oxidative metabolism in postischemic myocardium, Circ. Res. 71:567–576.PubMedGoogle Scholar
  4. Dunphy G. and Ely D., 1990, Decreased storage stability of creatine kinase in a cardiac reperfusion solution, Clin. Chem. 36:778–780.PubMedGoogle Scholar
  5. Goldhaber J.I. and Weiss J.N., 1992, Oxygen free radicals and cardiac reperfusion abnormalities, Hypertension 20:118–127.PubMedGoogle Scholar
  6. Hearse D.J. and Bolli R., 1992, Reperfusion induced injury: manifestations, mechanisms, and clinical relevance, Cardiovasc. Res. 26:101–108.PubMedCrossRefGoogle Scholar
  7. Kloner R.A., Przyklenk K. and Whittaker P., 1989, Deleterious effects of oxygen radicals in ischemia/ reperfusion. Resolved and unresolved issues, Circulation 80:1115–1127.CrossRefGoogle Scholar
  8. Lefer A.M., 1987, Interaction between myocardial depressant factor and vasoactive mediators with ischemia and shock, Am. J. Physiol. 252:R193–R205.PubMedGoogle Scholar
  9. Oliver I.T., 1966, A spectrophotometric method for the determination of creatine Phosphokinase and myokinase, Biochem. J. 61:116–122.Google Scholar
  10. Osbakken M.D., van Beek J.H.G.M. and Zhang D., 1994, Cardiac resuscitation: Role of N2 and 02, J. Am. Coll. Cardiol, Abstract Volume, 43rd Annual Scientific Session. 152A.Google Scholar
  11. Reimer K.A., Tanaka M., Murry C.E., Richard V.J. and Jennings R.B., 1990, Evaluation of free radical injury in myocardium, Toxicol. Pathol. 18:470–80.PubMedGoogle Scholar
  12. Snedecor G.W and Cochran W.G., 1980. “Statistical Methods”, The Iowa State University Press, Ames, IA, USA.Google Scholar
  13. Wixson S.K., White W.J., Hughes H.C., Lang CM. and Marshall W.K., 1987, A comparison of pentobarbital, fentanyl-droperidol, ketamine-xylazine and ketamine-diazepam anesthesia in adult male rats, Lab. Anim. Sci. 37:726–749.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1996

Authors and Affiliations

  • D. R. Rutgers
    • 1
  • J. H. G. M. van Beek
    • 1
  • M. D. Osbakken
    • 2
    • 3
  1. 1.Laboratory for Physiology, Institute for Cardiovascular Research (ICaR-VU)Vrije UniversiteitAmsterdamThe Netherlands
  2. 2.Bristol—Myers SquibbPrincetonUSA
  3. 3.University of PennsylvaniaPhiladelphiaUSA

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