Measurement of the Oxygenation Status of the Isolated Perfused Rat Heart Using Near Infrared Detection

  • Johannes H. G. M. van BeekEmail author
  • Mary D. Osbakken
  • Britton Chance
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 388)


The oxygenation status of isolated hearts has often been measured using optical detection methods for the visible range of the spectrum (Tamura et al., 1989). To the best of our knowledge there are no reports on measurement of myoglobin oxygenation using detection methods in the near-infrared region of the spectrum. On the other hand, equipment for near-infrared measurements on tissue is widely available and is in some instances quite inexpensive. Therefore we investigated in the present study whether near-infrared (NIR) detection at two wavelengths provides a way to conveniently determine myoglobin oxygenation in an isolated organ as small as the saline-perfused rat heart.


Light Guide Dual Wavelength Perfusion Flow Oxygenation Signal Mitochondrial Oxygen Consumption 
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Copyright information

© Plenum Press, New York 1996

Authors and Affiliations

  • Johannes H. G. M. van Beek
    • 1
    Email author
  • Mary D. Osbakken
    • 2
    • 3
  • Britton Chance
    • 2
    • 3
  1. 1.Laboratory for Physiology, Institute for Cardiovascular ResearchFree UniversityAmsterdamThe Netherlands
  2. 2.Laboratory for Physiology Institute for Cardiovascular ResearchFree UniversityAmsterdamThe Netherlands
  3. 3.Department of Biochemistry and BiophysicsUniversity of PennsylvaniaPhiladelphiaUSA

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