Oxygen Dependent Quenching of Phosphorescence

A Status Report
  • David F. Wilson
  • Sergei Vinogradov
  • Leu-Wei Lo
  • Lu Huang
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 388)


Oxygen dependent quenching of phosphorescence has now been in use as a method for measuring oxygen for about 10 years. As expected for a new technique, these years have seen a rapid evolution of every aspect of the method, and as it matures the full power of the technique is becoming apparent. Several properties of oxygen measurements by phosphorescence which are of great value compared to other available method for measuring oxygen were established early, including; its very rapid response time (msec), accuracy to low oxygen pressures (< 0.1 Torr), wide dynamic range, and applicability to measurements in tissue in vivo. The limits of the method have been determined in part by the available phosphorescent oxygen probes, the best of which has been a series of Pd-porphyrins. These probes provide a good measure of oxygen, but the excitation light needed to be in the part of the spectrum which is strongly absorbed by pigments in tissue and this restricted oxygen measurements to the surface about 1 mm of tissue.


Oxygen Pressure Excitation Light Oxygen Measurement Newborn Piglet Oxygen Probe 
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Copyright information

© Plenum Press, New York 1996

Authors and Affiliations

  • David F. Wilson
    • 1
  • Sergei Vinogradov
    • 1
  • Leu-Wei Lo
    • 1
  • Lu Huang
    • 1
  1. 1.Department of Biochemistry and BiophysicsUniversity of PennsylvaniaPhiladelphiaUSA

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