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Oxygen Dependent Quenching of Phosphorescence

A Status Report

  • Chapter
Oxygen Transport to Tissue XVII

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 388))

Abstract

Oxygen dependent quenching of phosphorescence has now been in use as a method for measuring oxygen for about 10 years. As expected for a new technique, these years have seen a rapid evolution of every aspect of the method, and as it matures the full power of the technique is becoming apparent. Several properties of oxygen measurements by phosphorescence which are of great value compared to other available method for measuring oxygen were established early, including; its very rapid response time (msec), accuracy to low oxygen pressures (< 0.1 Torr), wide dynamic range, and applicability to measurements in tissue in vivo. The limits of the method have been determined in part by the available phosphorescent oxygen probes, the best of which has been a series of Pd-porphyrins. These probes provide a good measure of oxygen, but the excitation light needed to be in the part of the spectrum which is strongly absorbed by pigments in tissue and this restricted oxygen measurements to the surface about 1 mm of tissue.

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania, 19104, USA

    David F. Wilson, Sergei Vinogradov, Leu-Wei Lo & Lu Huang

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  1. David F. Wilson
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  2. Sergei Vinogradov
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  3. Leu-Wei Lo
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  4. Lu Huang
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Editors and Affiliations

  1. Academic Medical Center, Amsterdam, The Netherlands

    C. Ince  & J. Kesecioglu  & 

  2. University of Istanbul, Istanbul, Turkey

    L. Telci  & K. Akpir  & 

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© 1996 Plenum Press, New York

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Wilson, D.F., Vinogradov, S., Lo, LW., Huang, L. (1996). Oxygen Dependent Quenching of Phosphorescence. In: Ince, C., Kesecioglu, J., Telci, L., Akpir, K. (eds) Oxygen Transport to Tissue XVII. Advances in Experimental Medicine and Biology, vol 388. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0333-6_12

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  • DOI: https://doi.org/10.1007/978-1-4613-0333-6_12

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-8002-3

  • Online ISBN: 978-1-4613-0333-6

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