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Cloning and Expression of the Gene Encoding a Novel Proteinase from TritiraChium Album Limber

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Subtilisin Enzymes

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 379))

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Summary

We have isolated the cDNA and the genomic clones encoding a novel serine proteinase, named proteinase T, from the fungus Tritirachium album Limber. The coding region of the gene is interrupted by two introns. The amino acid sequence of proteinase T as deduced from the nucleotide sequence is about 56% identical to that of proteinase K. Four cysteines are present in the mature proteinase, probably in the form of disulfide bonds.

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Authors and Affiliations

  1. Amgen Center, Amgen Inc., Thousand Oaks, California, 91320, USA

    Babru Sama, Tom Boone, Barbara Karan, Kenneth Chen, Raj Sachdev & Tsutomo Arakawa

Authors
  1. Babru Sama
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  2. Tom Boone
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  3. Barbara Karan
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  4. Kenneth Chen
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  5. Raj Sachdev
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  6. Tsutomo Arakawa
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Editor information

Editors and Affiliations

  1. Genecor International, Inc., South San Francisco, California, USA

    Richard Bott

  2. European Molecular Biology Laboratory, Hamburg, Germany

    Christian Betzel

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© 1996 Plenum Press, New York

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Sama, B., Boone, T., Karan, B., Chen, K., Sachdev, R., Arakawa, T. (1996). Cloning and Expression of the Gene Encoding a Novel Proteinase from TritiraChium Album Limber. In: Bott, R., Betzel, C. (eds) Subtilisin Enzymes. Advances in Experimental Medicine and Biology, vol 379. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0319-0_10

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  • DOI: https://doi.org/10.1007/978-1-4613-0319-0_10

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-306-45108-9

  • Online ISBN: 978-1-4613-0319-0

  • eBook Packages: Springer Book Archive

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