Cryopreservation of Blastocysts

  • Yves Menezo
  • Denny Sakkas
  • Anna Veiga
Part of the Proceedings in the Serono Symposia USA Series book series (SERONOSYMP)


Based on observations collected from IVM-IVF bovine blastocysts, we developed and modified cryopreservation programs for human co-cultured blastocysts. We have used glycerol as cryoprotectant, and sucrose. We were surprised to observe that the in vitro grown blastocysts are rather more sensitive to high concentrations of cryoprotectants. Our current protocol includes cryoprotectant addition in two steps and removal in two steps. The final concentration of glycerol, however, has been decreased to 9% (i.e., 1.125 M).


Implantation Rate Blastocyst Formation Blastocyst Transfer Human Blastocyst Sequential Medium 
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© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Yves Menezo
  • Denny Sakkas
  • Anna Veiga

There are no affiliations available

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