Abstract
Based on observations collected from IVM-IVF bovine blastocysts, we developed and modified cryopreservation programs for human co-cultured blastocysts. We have used glycerol as cryoprotectant, and sucrose. We were surprised to observe that the in vitro grown blastocysts are rather more sensitive to high concentrations of cryoprotectants. Our current protocol includes cryoprotectant addition in two steps and removal in two steps. The final concentration of glycerol, however, has been decreased to 9% (i.e., 1.125 M).
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Menezo, Y., Sakkas, D., Veiga, A. (2001). Cryopreservation of Blastocysts. In: Gardner, D.K., Lane, M. (eds) ART and the Human Blastocyst. Proceedings in the Serono Symposia USA Series. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-0149-3_15
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DOI: https://doi.org/10.1007/978-1-4613-0149-3_15
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4612-6540-5
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