Abstract
The challenge to reduce the incidence of high-order multiple gestations resulting from assisted reproduction technology has revived interest in the transfer of blastocysts, as orginally attempted by Bolton et al. (1). Higher implantation rates following blastocyst transfer enable transfer of fewer embryos while maintaining high pregnancy rates; however, suboptimal extended culture systems and intrinsic embryo quality greatly influence development of a cleavage stage embryo to the blastocyst stage. In fact, a cohort of embryos may have no survival on Day 5. The dilemma, therefore, concerns which patients and which embryos are suited to blastocyst transfer.
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References
Bolton VN, Wren ME, Parsons JH. Pregnancies after in vitro fertilization and transfer of human blastocysts. Fertil Steril 1991;55:830–32.
Schulman A, Ben-Nun I, Ghetler Y, Kaneti H, Shilon M, Beyth Y. Relationship between embryo morphology and implantation rate after in vitro fertilization treatment in conception cycles. Fertil Steril 1993;60 (1):123–26.
Racowsky C, Jackson KV, Cekleniak NA, Fox JH, Hornstein MD, Ginsburg ES. The number of eight-cell embryos is a key determinant for selecting day 3 or day 5 transfer. Fertil Steril 2000;73(3):558–64.
Alikani M, Cohen J, Tomkin G, Garrisi J, Mack C, Scott RT. Human embryo fragmentation in vitro and its implications for pregnancy and implantation. Fertil Steril 1999;71(5):83–2.
Cekleniak NA, Jackson KV, Nurredin A, Shen S, de los Santos MJ, Pillar M, et al. Reduced membrane definition of blastomeres on day 3 identifies embryos resulting in higher ongoing pregnancy rates after transfer on day 5 (abstract P–051). Am Soc Reprod Med 1999;72(3)(Suppl. 1).
Braude PR, Bolton V, Moore S. Human gene expression first occurs between the four and eight cell stages of preimplantation development. Nature 1988;332:459–61.
Asakura H, Stehlik EF, Wise L, Winchester-Peden K, Stehlik JC, Katayama KP. Correlation between preimplantation genetic diagnosis (PGD) of human embryos by multicolor flourescent in situ hybridization (FISH) and blastocyst development in vitro (abstract 483). J Soc Gynecol Investig 2000;7(Suppl. 1).
Gardner DK, Schoolcraft WB, Wagley L, Schlenker T, Stevens J, Hesla J. A prospective randomized trial of blastocyst culture and transfer in in-vitro fertilization. Hum Reprod 1998;13(12):3434–40.
Del Marek MA, Langley M, Gardner DK, Confer N, Doody KM, Doody KJ. Introduction of blastocyst culture and transfer for all patients in an in vitro fertilization program. Fertil Steril 1999;72(6):1035–40.
Behr B, Fisch JD, Racowsky C, Miller K, Pool TB, Milki AA. Blastocyst-ET and monozygotic twinning. J Assist Reprod Genet 2000;17:349–51.
Cohen J. Eisner C. Kort H. Malter H. Massey J. Mayer MP. et al. Impairment of the hatching process following IVF in the human and improvemnet of implantation by assisting hatching using micromanipulation. Hum Reprod 1990;5:7–13.
Carrillo AJ, Lane B, Pridham DD. Risch P, Pool TB, Silverman IH. et al. Improved clinical outcomes for in vitro fertilization with delay of embryo transfer from 48 to 72 hours after oocyte retrieval: use of glucose- and phosphate-free media. Fertil Steril 1998;69:329–34.
Jones GM, Trounson AO, Lolatgis N, Wood C. Factors affecting the success of human blastocyst development and pregnancy following in vitro fertilization and embryo transfer. Fertil Steril 1998;70(6):1022–29.
Shoukir Y, Chardonnens D, Campana A, Sakkas D. Blastocyst development from supernumerary embryos after intracytoplasmic sperm injection: a paternal influence? HumReprod 1998;13(6):1632–37.
Dumoulin JC, Coonen E, Bras M, Van Wissen LC, Ignoul-Vanvuchele R, Bergers-Jansen JM, Derhaag JG, et al. Comparison of in-vitro development of embryos originating from either conventional in-vitro fertilization or intracytoplasmic sperm injection. Hum Reprod 2000;15(2):402–9.
Bar-Hava I, Ashkenazi J, Shelef M, Schwartz A, Brenguaz M, Feldber Orvieto R, et al. Morphology and clinical outcomes of embryos after in vitro fertilization are superior to those after intracytoplasmic sperm injection. Fertil Steril 1997;68(4):653–57.
Janny L, Menezo YJ. Evidence for a strong patern al effect on human preimplantation embryo development and blastocyst formation. Mol Reprod Dev 1994;38(1):3–2.
Bolton VN, Hawes SM, Taylor CT, Parsons JH. Development of spare human preimplantation embryos in vitro; an analysis of the correlations among gross morphology, cleavage rates, and development to the blastocyst. J In Vitro Fert Embryo Transf 1989;6:30–35.
Ziebe S, Petersen K, Lindenberg S, Andersen AG, Gabrielsen A, Andersen AN. Embryo morphology or cleavage stage: how to select the best embryos for transfer after in-vitro fertilization. Hum Reprod 1997;12(7):1545–49.
Ducibella T, Albertini DF, Anderson E, Biggers JD. The preimplantation mammalian embryo: characterization of intercellular junctions and their appearance during development. Dev Biol 1975;45:231–50.
Veeck LL. An atlas of human gametes and conceptuses. New York: Parthenon Publishing, 1999:64.
Shoukir Y, Chardonnens D, Campana A, Bischof P, Sakkas D. The rate of development and time of transfer play different roles in influencing the viability of human blastocysts. Hum Reprod 1998;13(13):676–81.
Scholtes MCW, Zeilmaker GH. Blastocyst transfer in day–5 embryo transfer depends primarilyon the number of oocytes retrieved and not on age. Fertil Steril 1998;69(1):78–83.
Huisman GJ, Fauser BCJM, Eijkemans MJC, Pieters MHEC. Implantation rates after in vitro fertilization and transfer of a maximum of two embryos that have undergone three to five days in culture. Fertil Steril 2000;73(1):117–22.
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Cekleniak, N.A., Jackson, K.V., Racowsky, C. (2001). Predictors of Viability of the Human Blastocyst. In: Gardner, D.K., Lane, M. (eds) ART and the Human Blastocyst. Proceedings in the Serono Symposia USA Series. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-0149-3_12
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DOI: https://doi.org/10.1007/978-1-4613-0149-3_12
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